全氟辛酸暴露和热应激对青春期后后备母猪生产性能和肝脏健康生物标志物的影响

IF 2.9 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Journal of animal science Pub Date : 2025-10-11 DOI:10.1093/jas/skaf348

B M Buol, C Antwi-Boasiako, E J Mayorga, M E Gonzalez-Alvarez, L H Baumgard, A F Keating

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引用次数: 0

摘要

目的是评估饲粮全氟辛酸（PFOA）和热应激（HS）对猪生长性能和肝脏健康生物标志物的影响。采用2 × 2因子设计，将48头体重为164.0±11.6 kg的青春期后母猪随机分为4组处理：1)热中性（TN）对照（n = 12; TN- con）、2)TN + PFOA （n = 12; TN-PFOA）、3)HS对照（n = 12; HS- con）或4)HS + PFOA (n = 12; HS-PFOA)，共进行3个试验期(P)。在P1 （3 d）期间，猪在TN（20.3±0.1°C）条件下饲养，并收集基线数据。在P2 （15 d）期间，HS- con和HS- pfoa猪暴露于周期性HS(29.3±0.1 ~ 31.9±0.8°C)，而TN- con和TN- pfoa仍处于TN条件下。在P2期每日1次（0800 h）给予Altrenogest以同步发情。在P3 （4 d）时，停用阿替诺肽诱导发情，实验处理不变。在P2和P3期间，每天1次（0800 h）口服PFOA (70 ng/kg BW)，在P3结束时对所有猪实施安乐死。与TN相比，HS在P2（分别为0.34°C、5.67°C和22 bpm； P < 0.01）和P3（分别为0.26°C、6.96°C和22 bpm； P < 0.01）期间提高了直肠温度、皮肤温度和呼吸速率，而这些仅受PFOA的轻微影响。P2和P3期，HS显著降低采食量和平均日增重，PFOA有降低采食量和平均日增重的趋势；在P2和P3阶段，TN条件（410 g/d, P≤0.08和320 g/d， P≤0.09）的影响最为显著。HS降低了P2和P3循环碱性磷酸酶和丙氨酸转氨酶（P≤0.01），升高了P2循环γ -谷氨酰转移酶（P < 0.01），但PFOA对这些酶无影响。饮食治疗或环境对其他肝脏健康生物标志物几乎没有影响。HS降低了肝脏、肺和肾脏的绝对和相对重量，PFOA降低了肺的绝对重量（11%;P < 0.05）和相对重量（0.06%;P = 0.09）。综上所述，PFOA和HS各自影响食欲和生长，但PFOA和HS的影响似乎不是相加的。

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Effects of perfluorooctanoic acid exposure and heat stress on performance and liver health biomarkers in post-pubertal gilts

Objectives were to evaluate how dietary perfluorooctanoic acid (PFOA) and heat stress (HS) affect growth performance and liver health biomarkers in pigs. Crossbred post-pubertal gilts (n = 48; 164.0 ± 11.6 kg body weight [BW]) were randomly assigned to 1 of 4 treatments in a 2 × 2 factorial design: 1) thermoneutral (TN) control (n = 12; TN-CON), 2) TN and PFOA (n = 12; TN-PFOA), 3) HS control (n = 12; HS-CON), or 4) HS and PFOA (n = 12; HS-PFOA), and enrolled in 3 experimental periods (P). During P1 (3 d), pigs were housed in TN conditions (20.3 ± 0.1 °C) and baseline data were collected. During P2 (15 d), HS-CON and HS-PFOA pigs were exposed to cyclical HS (29.3 ± 0.1 to 31.9 ± 0.8 °C), while TN-CON and TN-PFOA remained in TN conditions. Altrenogest was administered once daily (0800 h) during P2 to synchronize estrus. In P3 (4 d), Altrenogest was withdrawn to induce estrus, while experimental treatments remained the same. PFOA (70 ng/kg BW) was orally administered once daily (0800 h) during P2 and P3, and all pigs were euthanized at the end of P3. HS increased rectal temperature, skin temperature, and respiration rate compared to TN counterparts during P2 (0.34 °C, 5.67 °C, and 22 bpm, respectively; P < 0.01) and P3 (0.26 °C, 6.96 °C, and 22 bpm, respectively; P < 0.01), and these were only marginally influenced by PFOA. During P2 and P3, HS markedly decreased feed intake (FI) and average daily gain (ADG), and PFOA tended to decrease FI and ADG; an effect most pronounced in TN conditions (410 g/d; P ≤ 0.08 and 320 g/d; P ≤ 0.09, respectively) during P2 and P3. HS decreased circulating alkaline phosphatase and alanine aminotransferase in P2 and P3 (P ≤ 0.01), and increased gamma-glutamyl transferase in P2 (P < 0.01), but these enzymes were unaffected by PFOA. There was little to no dietary treatment or environmental effects on other liver health biomarkers. HS decreased absolute and relative liver, lung, and kidney weights, and PFOA decreased absolute lung weight (11%; P < 0.05) and relative lung weight (0.06%; P = 0.09). In summary, PFOA and HS independently compromised appetite and growth, but the effects of PFOA and HS do not appear to be additive.

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来源期刊

Journal of animal science 农林科学-奶制品与动物科学

CiteScore

4.80

自引率

12.10%

发文量

1589

审稿时长

3 months

期刊介绍： The Journal of Animal Science (JAS) is the premier journal for animal science and serves as the leading source of new knowledge and perspective in this area. JAS publishes more than 500 fully reviewed research articles, invited reviews, technical notes, and letters to the editor each year. Articles published in JAS encompass a broad range of research topics in animal production and fundamental aspects of genetics, nutrition, physiology, and preparation and utilization of animal products. Articles typically report research with beef cattle, companion animals, goats, horses, pigs, and sheep; however, studies involving other farm animals, aquatic and wildlife species, and laboratory animal species that address fundamental questions related to livestock and companion animal biology will be considered for publication.