Jingyi Zheng, Su Ryun Choi, Yue Jing, Wenjun Zhang, Yan Sun, Xiaonan Li, Yong Pyo Lim
{"title":"BrGSL-OHa启动子的自然变异有助于油菜叶片中葡萄糖糖苷转化为原甘油素。","authors":"Jingyi Zheng, Su Ryun Choi, Yue Jing, Wenjun Zhang, Yan Sun, Xiaonan Li, Yong Pyo Lim","doi":"10.3389/fpls.2025.1654238","DOIUrl":null,"url":null,"abstract":"<p><p>Glucosinolates (GSLs) are sulfur-rich secondary metabolites that play important roles in human health, plant defenses against pathogens and insects, and flavor. The genetic architecture of GSL biosynthesis in <i>Brassica rapa</i> L. remains poorly understood despite several mapping and gene prediction studies. This study conducted a conventional quantitative trait locus (QTL) analysis to identify putative genes regulating GSL biosynthesis in <i>B. rapa</i> in two field trials. Four consensus QTL clusters were identified for various GSL compounds. Six QTLs exhibited effects of QTL-environment interactions (Q×E), reflecting the genetic variation underlying phenotypic plasticity. QTL-Cluster2 and QTL-Cluster3 on chromosome A03 represented two genetically stable regions for major aliphatic GSLs (Ali-GSLs) without Q×E effects. Interestingly, variation in the expression of <i>BrGSL-OHa</i>, rather than gene sequence variation, explained the association between QTL-Cluster2 and gluconapin and progoitrin accumulation in <i>B. rapa</i>. Further function analysis indicated that the lack of an MYB binding site in the oil-type <i>B. rapa BrGSL-OHa</i> promoter region represented a rare non-functional allele among <i>B. rapa</i> genotypes, which prevented binding with the MYB transcription factor BrMYB29b, thereby repressing <i>BrGSL-OHa</i> transcription and inhibiting progoitrin biosynthesis. This study provides new insights regarding the molecular regulatory mechanism of GSL biosynthesis in <i>B. rapa</i>.</p>","PeriodicalId":12632,"journal":{"name":"Frontiers in Plant Science","volume":"16 ","pages":"1654238"},"PeriodicalIF":4.1000,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12504251/pdf/","citationCount":"0","resultStr":"{\"title\":\"A naturally occurring promoter variation of <i>BrGSL-OHa</i> contributes to the conversion of gluconapin to progoitrin in <i>Brassica rapa</i> L. leaves.\",\"authors\":\"Jingyi Zheng, Su Ryun Choi, Yue Jing, Wenjun Zhang, Yan Sun, Xiaonan Li, Yong Pyo Lim\",\"doi\":\"10.3389/fpls.2025.1654238\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Glucosinolates (GSLs) are sulfur-rich secondary metabolites that play important roles in human health, plant defenses against pathogens and insects, and flavor. The genetic architecture of GSL biosynthesis in <i>Brassica rapa</i> L. remains poorly understood despite several mapping and gene prediction studies. This study conducted a conventional quantitative trait locus (QTL) analysis to identify putative genes regulating GSL biosynthesis in <i>B. rapa</i> in two field trials. Four consensus QTL clusters were identified for various GSL compounds. Six QTLs exhibited effects of QTL-environment interactions (Q×E), reflecting the genetic variation underlying phenotypic plasticity. QTL-Cluster2 and QTL-Cluster3 on chromosome A03 represented two genetically stable regions for major aliphatic GSLs (Ali-GSLs) without Q×E effects. Interestingly, variation in the expression of <i>BrGSL-OHa</i>, rather than gene sequence variation, explained the association between QTL-Cluster2 and gluconapin and progoitrin accumulation in <i>B. rapa</i>. Further function analysis indicated that the lack of an MYB binding site in the oil-type <i>B. rapa BrGSL-OHa</i> promoter region represented a rare non-functional allele among <i>B. rapa</i> genotypes, which prevented binding with the MYB transcription factor BrMYB29b, thereby repressing <i>BrGSL-OHa</i> transcription and inhibiting progoitrin biosynthesis. 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A naturally occurring promoter variation of BrGSL-OHa contributes to the conversion of gluconapin to progoitrin in Brassica rapa L. leaves.
Glucosinolates (GSLs) are sulfur-rich secondary metabolites that play important roles in human health, plant defenses against pathogens and insects, and flavor. The genetic architecture of GSL biosynthesis in Brassica rapa L. remains poorly understood despite several mapping and gene prediction studies. This study conducted a conventional quantitative trait locus (QTL) analysis to identify putative genes regulating GSL biosynthesis in B. rapa in two field trials. Four consensus QTL clusters were identified for various GSL compounds. Six QTLs exhibited effects of QTL-environment interactions (Q×E), reflecting the genetic variation underlying phenotypic plasticity. QTL-Cluster2 and QTL-Cluster3 on chromosome A03 represented two genetically stable regions for major aliphatic GSLs (Ali-GSLs) without Q×E effects. Interestingly, variation in the expression of BrGSL-OHa, rather than gene sequence variation, explained the association between QTL-Cluster2 and gluconapin and progoitrin accumulation in B. rapa. Further function analysis indicated that the lack of an MYB binding site in the oil-type B. rapa BrGSL-OHa promoter region represented a rare non-functional allele among B. rapa genotypes, which prevented binding with the MYB transcription factor BrMYB29b, thereby repressing BrGSL-OHa transcription and inhibiting progoitrin biosynthesis. This study provides new insights regarding the molecular regulatory mechanism of GSL biosynthesis in B. rapa.
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In an ever changing world, plant science is of the utmost importance for securing the future well-being of humankind. Plants provide oxygen, food, feed, fibers, and building materials. In addition, they are a diverse source of industrial and pharmaceutical chemicals. Plants are centrally important to the health of ecosystems, and their understanding is critical for learning how to manage and maintain a sustainable biosphere. Plant science is extremely interdisciplinary, reaching from agricultural science to paleobotany, and molecular physiology to ecology. It uses the latest developments in computer science, optics, molecular biology and genomics to address challenges in model systems, agricultural crops, and ecosystems. Plant science research inquires into the form, function, development, diversity, reproduction, evolution and uses of both higher and lower plants and their interactions with other organisms throughout the biosphere. Frontiers in Plant Science welcomes outstanding contributions in any field of plant science from basic to applied research, from organismal to molecular studies, from single plant analysis to studies of populations and whole ecosystems, and from molecular to biophysical to computational approaches.
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