锰掺杂palladium@iridium纳米酶的原子壳工程：超灵敏横向流动免疫测定中增强过氧化物酶样活性的d波段优化

IF 13.2 1区工程技术 Q1 ENGINEERING, CHEMICAL

Chemical Engineering Journal Pub Date : 2025-10-10 DOI:10.1016/j.cej.2025.169492

Ting Tan, Qianqian Cao, Ming-Hui Duan, Junmin Li, Yafeng Xie, Min Tan, Xiwu Yin, JiKai Wang, Weiguo Wang

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引用次数: 0

摘要

侧流免疫分析法（LFIA）由于操作简单、检测效率高，在临床诊断、食品安全、环境保护等方面得到了广泛的应用。然而，当处理低水平的生物标志物时，LFIA的灵敏度仍达不到要求。本文合成了一种具有超薄铱（Ir）外壳（~2 nm）的锰掺杂核壳纳米酶（Pd@Ir-Mn），并将其用于LFIA的信号放大。通过掺杂其他金属（Mn, Fe, Co, Ni， Cu和Zn），调整了Ir的d波段中心。d波段中心与转换系数的关系呈火山图，mn掺杂Pd@Ir位于火山峰。最终将制备的Pd@Ir-Mn应用于LFIA （HT-Pd@Ir-Mn-LFIA）检测hCG，与传统的金纳米颗粒LFIA （AuNPs-LFIA）相比，灵敏度提高了41倍。该方法具有良好的特异性、稳定性、精密度和准确性，适用于临床检测。

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Atomic-shell engineering in manganese-doped palladium@iridium nanozymes: d-Band optimization for enhanced peroxidase-like activity in ultrasensitive lateral flow immunoassays

Lateral flow immunoassay (LFIA) has been widely used in clinical diagnosis, food safety, and environmental protection due to the simple operation and high detection efficiency. However, the sensitivity of LFIA still falls short of the requirements when dealing with low levels of biomarkers. In this work, a type of manganese-doped core-shell nanozyme (Pd@Ir-Mn) with an ultrathin iridium (Ir) shell (~2 nm) was synthesized and utilized for the signal amplification of LFIA. By doping with other metals (Mn, Fe, Co, Ni, Cu, and Zn), the d-band center of Ir was tuned. The relationship between the d-band center and the conversion coefficient followed a volcano plot, with Mn-doped Pd@Ir located at the peak of the volcano. The as-prepared Pd@Ir-Mn was finally applied to LFIA (HT-Pd@Ir-Mn-LFIA) for the detection of hCG, the sensitivity was improved by 41 times compared to the traditional gold nanoparticles based LFIA (AuNPs-LFIA). Moreover, this method exhibited good specificity, stability, precision, and accuracy, making it suitable for clinical detection.

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来源期刊

Chemical Engineering Journal 工程技术-工程：化工

CiteScore

21.70

自引率

9.30%

发文量

6781

审稿时长

2.4 months

期刊介绍： The Chemical Engineering Journal is an international research journal that invites contributions of original and novel fundamental research. It aims to provide an international platform for presenting original fundamental research, interpretative reviews, and discussions on new developments in chemical engineering. The journal welcomes papers that describe novel theory and its practical application, as well as those that demonstrate the transfer of techniques from other disciplines. It also welcomes reports on carefully conducted experimental work that is soundly interpreted. The main focus of the journal is on original and rigorous research results that have broad significance. The Catalysis section within the Chemical Engineering Journal focuses specifically on Experimental and Theoretical studies in the fields of heterogeneous catalysis, molecular catalysis, and biocatalysis. These studies have industrial impact on various sectors such as chemicals, energy, materials, foods, healthcare, and environmental protection.