印度水稻对根结线虫(Meloidogyne graminicola)的抗性评价:来自田间和组织病理学研究的见解。

IF 5 1区 农林科学 Q1 AGRONOMY
Rice Pub Date : 2025-10-08 DOI:10.1186/s12284-025-00852-x
Vedant Gautam, Vibhootee Garg, Hivre Anand Dashrath, Nitesh Meena, Nikhil Kumar Singh, Ashish Kumar, Nethi Somasekhar, R K Singh
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引用次数: 0

摘要

水稻(Oryza sativa L.)是全球一半以上人口的主要作物,但其种植面临着生物胁迫的重大威胁,特别是根结线虫(Meloidogyne spp.)。其中,稻瘟病菌对水稻种植区构成重大挑战,导致大量产量损失。通过盆栽和田间对照试验(2023年和2024年),对348个水稻品种对稻瘟病菌的抗性进行了评价。根据胆指数对品种进行分类,揭示了从高度敏感到高度抗性的易感谱。值得注意的是,JR-1124和JR-403表现出较高的瘿指数,而RP-5219-9-7-3-2-1-1、NPT-10、MTU 1390 (IR17M1172)、Kushiari、RP 6750-RMS-2-23-67-91、Sonkharchi、Sugandha-3、HRT-183和HR-12表现出显著的抗性。抗性水稻的PAL、POX和总酚含量在各时间段均显著高于抗性水稻,表明抗性水稻对谷草曲线虫具有较强的生化防御反应。包括共聚焦显微镜在内的先进技术揭示了对M. graminicola感染的不同组织病理学反应,易感水稻品种表现出广泛的巨细胞形成和根组织降解,而抗性水稻品种表现出限制巨细胞发育,增强胼胝质沉积,并保持维管完整性-突出了抵抗线虫入侵的强大防御机制。这一发现强调了培育项目在增强水稻抗性性状、促进可持续农业实践和改善粮食安全方面的潜力。随着线虫种群的进化,正在进行的研究对于调整育种策略和保持对水稻生产系统中这一重要害虫的有效管理至关重要。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Evaluation of Resistance in Indian Rice to Root-Knot Nematode (Meloidogyne graminicola): Insights from Field and Histopathological Studies.

Rice (Oryza sativa L.) is a staple crop for over half of the global population, yet its cultivation faces significant threats from biotic stresses, particularly root-knot nematodes (Meloidogyne spp.). Among these, M. graminicola poses a major challenge in rice-growing regions, leading to substantial yield losses. This study evaluated the resistance of 348 rice varieties to M. graminicola through controlled pot and field experiments over two years (2023 and 2024). Varieties were classified based on gall index, revealing a spectrum of susceptibility from highly susceptible to highly resistant. Notably, varieties such as JR-1124 and JR-403 exhibited high gall index, while others like RP-5219-9-7-3-2-1-1, NPT-10, MTU 1390 (IR17M1172), Kushiari, RP 6750-RMS-2-23-67-91, Sonkharchi, Sugandha-3, HRT-183, and HR-12 demonstrated significant resistance. Resistant rice genotypes exhibited significantly higher PAL, POX, and total phenolic content at all intervals, indicating a strong biochemical defense response against Meloidogyne graminicola. Advanced techniques, including confocal microscopy, revealed distinct histopathological responses to M. graminicola infection, with susceptible rice varieties exhibiting extensive giant cell formation and root tissue degradation, while a resistant variety displayed restricted giant cell development, enhanced callose deposition, and maintained vascular integrity-highlighting robust defense mechanisms against nematode invasion. The findings underscore the potential for breeding programs to enhance resistance traits in rice, contributing to sustainable agricultural practices and improved food security. As nematode populations evolve, ongoing research is essential to adapt breeding strategies and maintain effective management of this significant pest in rice production systems.

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来源期刊
Rice
Rice AGRONOMY-
CiteScore
10.10
自引率
3.60%
发文量
60
审稿时长
>12 weeks
期刊介绍: Rice aims to fill a glaring void in basic and applied plant science journal publishing. This journal is the world''s only high-quality serial publication for reporting current advances in rice genetics, structural and functional genomics, comparative genomics, molecular biology and physiology, molecular breeding and comparative biology. Rice welcomes review articles and original papers in all of the aforementioned areas and serves as the primary source of newly published information for researchers and students in rice and related research.
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