Jacek Sroka, Jacek Zwoliński, Ewa Bilska-Zając, Angelina Wójcik Fatla, Jacek Karamon, Jolanta Małgorzata Zdybel, Weronika Wiktoria Korpysa-Dzirba, Małgorzata Samorek-Pieróg, Weronika Piotrowska, Tomasz Cencek
{"title":"波兰犊牛粪便样本中的十二指肠贾第虫。","authors":"Jacek Sroka, Jacek Zwoliński, Ewa Bilska-Zając, Angelina Wójcik Fatla, Jacek Karamon, Jolanta Małgorzata Zdybel, Weronika Wiktoria Korpysa-Dzirba, Małgorzata Samorek-Pieróg, Weronika Piotrowska, Tomasz Cencek","doi":"10.2478/jvetres-2025-0052","DOIUrl":null,"url":null,"abstract":"<p><strong>Introduction: </strong><i>Giardia duodenalis</i> is the prevalent parasitic protozoan responsible for diarrhoeal disease in humans and animals. This study aimed to determine the prevalence and assemblages of this parasite present in cattle in Poland.</p><p><strong>Material and methods: </strong>Faecal samples were collected from 1,602 cattle up to 4 months old, bred on 267 farms located in all 16 voivodeships of Poland. Extraction of DNA was performed using a modified alkaline and a heat lysis method. Molecular detection of <i>β-giardin</i> gene by PCR and sequence analysis were performed.</p><p><strong>Results: </strong>In 174 of 1,602 (10.9%) tested cattle and in 89 of 267 examined herds (33.3%), <i>G. duodenalis</i> DNA was detected. The highest prevalence of <i>Giardia</i> in cattle was found in the Lubelskie (25%), Mazowieckie (21.3%) and Zachodniopomorskie (20.4%) voivodeships. In other regions, the prevalence did not exceed 10%. The number of <i>Giardia</i>-positive cattle decreased with animal age. The most frequently identified assemblage in cattle was E (89.2%), and A and B were detected more seldom in 9.0% and 1.8% of cattle, respectively. No significant differences in positive results were observed in cattle depending on production purpose.</p><p><strong>Conclusion: </strong>The study results showed a high prevalence of <i>Giardia</i> in cattle in Poland. The presence of the <i>Giardia</i> A and B assemblages indicates a potential zoonotic threat.</p>","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":"69 3","pages":"439-445"},"PeriodicalIF":1.5000,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12503212/pdf/","citationCount":"0","resultStr":"{\"title\":\"<i>Giardia duodenalis</i> in faecal samples from calves in Poland.\",\"authors\":\"Jacek Sroka, Jacek Zwoliński, Ewa Bilska-Zając, Angelina Wójcik Fatla, Jacek Karamon, Jolanta Małgorzata Zdybel, Weronika Wiktoria Korpysa-Dzirba, Małgorzata Samorek-Pieróg, Weronika Piotrowska, Tomasz Cencek\",\"doi\":\"10.2478/jvetres-2025-0052\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Introduction: </strong><i>Giardia duodenalis</i> is the prevalent parasitic protozoan responsible for diarrhoeal disease in humans and animals. This study aimed to determine the prevalence and assemblages of this parasite present in cattle in Poland.</p><p><strong>Material and methods: </strong>Faecal samples were collected from 1,602 cattle up to 4 months old, bred on 267 farms located in all 16 voivodeships of Poland. Extraction of DNA was performed using a modified alkaline and a heat lysis method. Molecular detection of <i>β-giardin</i> gene by PCR and sequence analysis were performed.</p><p><strong>Results: </strong>In 174 of 1,602 (10.9%) tested cattle and in 89 of 267 examined herds (33.3%), <i>G. duodenalis</i> DNA was detected. The highest prevalence of <i>Giardia</i> in cattle was found in the Lubelskie (25%), Mazowieckie (21.3%) and Zachodniopomorskie (20.4%) voivodeships. In other regions, the prevalence did not exceed 10%. The number of <i>Giardia</i>-positive cattle decreased with animal age. The most frequently identified assemblage in cattle was E (89.2%), and A and B were detected more seldom in 9.0% and 1.8% of cattle, respectively. No significant differences in positive results were observed in cattle depending on production purpose.</p><p><strong>Conclusion: </strong>The study results showed a high prevalence of <i>Giardia</i> in cattle in Poland. The presence of the <i>Giardia</i> A and B assemblages indicates a potential zoonotic threat.</p>\",\"PeriodicalId\":17617,\"journal\":{\"name\":\"Journal of Veterinary Research\",\"volume\":\"69 3\",\"pages\":\"439-445\"},\"PeriodicalIF\":1.5000,\"publicationDate\":\"2025-09-24\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12503212/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Veterinary Research\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.2478/jvetres-2025-0052\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/9/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q2\",\"JCRName\":\"VETERINARY SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Veterinary Research","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.2478/jvetres-2025-0052","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/9/1 0:00:00","PubModel":"eCollection","JCR":"Q2","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}
Giardia duodenalis in faecal samples from calves in Poland.
Introduction: Giardia duodenalis is the prevalent parasitic protozoan responsible for diarrhoeal disease in humans and animals. This study aimed to determine the prevalence and assemblages of this parasite present in cattle in Poland.
Material and methods: Faecal samples were collected from 1,602 cattle up to 4 months old, bred on 267 farms located in all 16 voivodeships of Poland. Extraction of DNA was performed using a modified alkaline and a heat lysis method. Molecular detection of β-giardin gene by PCR and sequence analysis were performed.
Results: In 174 of 1,602 (10.9%) tested cattle and in 89 of 267 examined herds (33.3%), G. duodenalis DNA was detected. The highest prevalence of Giardia in cattle was found in the Lubelskie (25%), Mazowieckie (21.3%) and Zachodniopomorskie (20.4%) voivodeships. In other regions, the prevalence did not exceed 10%. The number of Giardia-positive cattle decreased with animal age. The most frequently identified assemblage in cattle was E (89.2%), and A and B were detected more seldom in 9.0% and 1.8% of cattle, respectively. No significant differences in positive results were observed in cattle depending on production purpose.
Conclusion: The study results showed a high prevalence of Giardia in cattle in Poland. The presence of the Giardia A and B assemblages indicates a potential zoonotic threat.
期刊介绍：
Journal of Veterinary Research (formerly Bulletin of the Veterinary Institute in Pulawy) is a quarterly that publishes original papers, review articles and short communications on bacteriology, virology, parasitology, immunology, molecular biology, pathology, toxicology, pharmacology, and biochemistry. The main emphasis is, however, on infectious diseases of animals, food safety and public health, and clinical sciences.
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