{"title":"木麻黄酚类物质的高效液相色谱分析：提取、定量及抗氧化评价。","authors":"Nourhene Aroua, Maher Boukhris, Wissal Ayouni, Sirine Choura, Mohamed Chamkha, Ridha Ben Salem, Rigane Ghayth","doi":"10.1080/14786419.2025.2569804","DOIUrl":null,"url":null,"abstract":"<p><p>The study was carried out to evaluate the phenolic compounds and antioxidant activity in the aqueous extract from leaves, stems and flowers of <i>Casuarina cunninghamiana</i> Miq. subsp. <i>Cunninghamiana</i> through ultrasound-assisted and maceration extractions from Sfax, Tunisia. The leaves exhibited the highest total phenolic and flavonoid contents. HPLC analysis identified 14 phenolic compounds across all extracts, including caffeic acid, epicatechin and verbascoside. Remarkably, catechin was present at the highest concentration in <i>C. cunninghamiana</i> flowers extracts. The antioxidant activity was assessed using the DPPH radical scavenging assay and FRAP assay. The leaves extract exhibited the strongest antioxidant activity, with an IC<b><sub>50</sub></b> value of 204.17 µg/mL in the DPPH assay and 197.5 µM BHT/g DW in the FRAP assay. These findings highlight the potential of <i>C. cunninghamiana</i> being studied for the first time in Tunisia as a natural source of bioactive compounds, with potential applications in biotechnology, pharmacology and natural product research.</p>","PeriodicalId":18990,"journal":{"name":"Natural Product Research","volume":" ","pages":"1-11"},"PeriodicalIF":1.6000,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"High-performance liquid chromatography analysis of phenolics in <i>Casuarina cunninghamiana</i>: extraction, quantification and antioxidant evaluation.\",\"authors\":\"Nourhene Aroua, Maher Boukhris, Wissal Ayouni, Sirine Choura, Mohamed Chamkha, Ridha Ben Salem, Rigane Ghayth\",\"doi\":\"10.1080/14786419.2025.2569804\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The study was carried out to evaluate the phenolic compounds and antioxidant activity in the aqueous extract from leaves, stems and flowers of <i>Casuarina cunninghamiana</i> Miq. subsp. <i>Cunninghamiana</i> through ultrasound-assisted and maceration extractions from Sfax, Tunisia. The leaves exhibited the highest total phenolic and flavonoid contents. HPLC analysis identified 14 phenolic compounds across all extracts, including caffeic acid, epicatechin and verbascoside. Remarkably, catechin was present at the highest concentration in <i>C. cunninghamiana</i> flowers extracts. The antioxidant activity was assessed using the DPPH radical scavenging assay and FRAP assay. The leaves extract exhibited the strongest antioxidant activity, with an IC<b><sub>50</sub></b> value of 204.17 µg/mL in the DPPH assay and 197.5 µM BHT/g DW in the FRAP assay. These findings highlight the potential of <i>C. cunninghamiana</i> being studied for the first time in Tunisia as a natural source of bioactive compounds, with potential applications in biotechnology, pharmacology and natural product research.</p>\",\"PeriodicalId\":18990,\"journal\":{\"name\":\"Natural Product Research\",\"volume\":\" \",\"pages\":\"1-11\"},\"PeriodicalIF\":1.6000,\"publicationDate\":\"2025-10-09\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Natural Product Research\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://doi.org/10.1080/14786419.2025.2569804\",\"RegionNum\":3,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"CHEMISTRY, APPLIED\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Natural Product Research","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1080/14786419.2025.2569804","RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"CHEMISTRY, APPLIED","Score":null,"Total":0}
High-performance liquid chromatography analysis of phenolics in Casuarina cunninghamiana: extraction, quantification and antioxidant evaluation.
The study was carried out to evaluate the phenolic compounds and antioxidant activity in the aqueous extract from leaves, stems and flowers of Casuarina cunninghamiana Miq. subsp. Cunninghamiana through ultrasound-assisted and maceration extractions from Sfax, Tunisia. The leaves exhibited the highest total phenolic and flavonoid contents. HPLC analysis identified 14 phenolic compounds across all extracts, including caffeic acid, epicatechin and verbascoside. Remarkably, catechin was present at the highest concentration in C. cunninghamiana flowers extracts. The antioxidant activity was assessed using the DPPH radical scavenging assay and FRAP assay. The leaves extract exhibited the strongest antioxidant activity, with an IC50 value of 204.17 µg/mL in the DPPH assay and 197.5 µM BHT/g DW in the FRAP assay. These findings highlight the potential of C. cunninghamiana being studied for the first time in Tunisia as a natural source of bioactive compounds, with potential applications in biotechnology, pharmacology and natural product research.
期刊介绍：
The aim of Natural Product Research is to publish important contributions in the field of natural product chemistry. The journal covers all aspects of research in the chemistry and biochemistry of naturally occurring compounds.
The communications include coverage of work on natural substances of land and sea and of plants, microbes and animals. Discussions of structure elucidation, synthesis and experimental biosynthesis of natural products as well as developments of methods in these areas are welcomed in the journal. Finally, research papers in fields on the chemistry-biology boundary, eg. fermentation chemistry, plant tissue culture investigations etc., are accepted into the journal.
Natural Product Research issues will be subtitled either ""Part A - Synthesis and Structure"" or ""Part B - Bioactive Natural Products"". for details on this , see the forthcoming articles section.
All manuscript submissions are subject to initial appraisal by the Editor, and, if found suitable for further consideration, to peer review by independent, anonymous expert referees. All peer review is single blind and submission is online via ScholarOne Manuscripts.
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