评估石榴籽油的潜在局部应用:安全性,抗炎活性和皮肤细胞模型的伤口愈合。

IF 5.3 2区 医学 Q2 IMMUNOLOGY
Eduardo M Costa, Manuela Machado, Manuela Pintado, Sara Silva
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引用次数: 0

摘要

石榴籽油(PSO)是石榴汁生产的副产品,在传统医学中具有悠久的作用,但其在皮肤应用方面的机械和药物潜力仍未被探索。本研究通过评估PSO对细胞代谢、抗炎特性和伤口愈合能力的影响,探讨了PSO在人角质形成细胞(HaCaT)和真皮成纤维细胞(HDFa)中的生物活性。结果显示,PSO在lps诱导的炎症条件下,当浓度达到500µg/mL时,无细胞毒作用,且能显著抑制促炎细胞因子(IL-6、IL-8、TNF-α)。此外,在24小时内,成纤维细胞的伤口愈合速度加快了约20%。虽然在诱导氧化应激下抗氧化活性有限,但PSO在基础条件下具有轻微的保护作用,脂肪酸谱显示富含pufa的成分,包括二高亚麻酸和果酸,可能与观察到的对细胞代谢的影响有关。总的来说,这些发现为PSO在皮肤再生和免疫调节方面的双重功能提供了新的见解,支持其作为先进局部治疗的生物活性成分的应用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Evaluating pomegranate seed oil for potential topical applications: safety, anti-inflammatory activity and wound healing in skin cell models.

Pomegranate seed oil (PSO), a by-product of pomegranate juice production, has a long-standing role in traditional medicine, yet is mechanistic and pharmaceutical potential for skin applications remains unexplored. This work explored the bioactivity of PSO in human keratinocytes (HaCaT) and dermal fibroblasts (HDFa) through evaluation of its impact upon cellular metabolism, anti-inflammatory properties and wound healing capacity. The data obtained showed that PSO exhibited no cytotoxic effects up to 500 µg/mL and significantly suppressed pro-inflammatory cytokines (IL-6, IL-8, TNF-α) under LPS-induced inflammatory conditions. Furthermore, wound closure in fibroblasts was accelerated by approximately 20% in a 24-h period. Although antioxidant activity was limited under induced oxidative stress, PSO exerted mild protective effects under basal conditions and fatty acid profiling revealed a PUFA-rich composition, including dihomolinolenic and punicic acids, potentially linked to the observed impact upon cellular metabolism. Overall, these findings offer novel insights into PSO's dual functionality in skin regeneration and immune modulation, supporting its application as a bioactive ingredient for advanced topical therapies.

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来源期刊
Inflammopharmacology
Inflammopharmacology IMMUNOLOGYTOXICOLOGY-TOXICOLOGY
CiteScore
8.00
自引率
3.40%
发文量
200
期刊介绍: Inflammopharmacology is the official publication of the Gastrointestinal Section of the International Union of Basic and Clinical Pharmacology (IUPHAR) and the Hungarian Experimental and Clinical Pharmacology Society (HECPS). Inflammopharmacology publishes papers on all aspects of inflammation and its pharmacological control emphasizing comparisons of (a) different inflammatory states, and (b) the actions, therapeutic efficacy and safety of drugs employed in the treatment of inflammatory conditions. The comparative aspects of the types of inflammatory conditions include gastrointestinal disease (e.g. ulcerative colitis, Crohn''s disease), parasitic diseases, toxicological manifestations of the effects of drugs and environmental agents, arthritic conditions, and inflammatory effects of injury or aging on skeletal muscle. The journal has seven main interest areas: -Drug-Disease Interactions - Conditional Pharmacology - i.e. where the condition (disease or stress state) influences the therapeutic response and side (adverse) effects from anti-inflammatory drugs. Mechanisms of drug-disease and drug disease interactions and the role of different stress states -Rheumatology - particular emphasis on methods of measurement of clinical response effects of new agents, adverse effects from anti-rheumatic drugs -Gastroenterology - with particular emphasis on animal and human models, mechanisms of mucosal inflammation and ulceration and effects of novel and established anti-ulcer, anti-inflammatory agents, or antiparasitic agents -Neuro-Inflammation and Pain - model systems, pharmacology of new analgesic agents and mechanisms of neuro-inflammation and pain -Novel drugs, natural products and nutraceuticals - and their effects on inflammatory processes, especially where there are indications of novel modes action compared with conventional drugs e.g. NSAIDs -Muscle-immune interactions during inflammation [...]
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