Eduardo M Costa, Manuela Machado, Manuela Pintado, Sara Silva
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Evaluating pomegranate seed oil for potential topical applications: safety, anti-inflammatory activity and wound healing in skin cell models.
Pomegranate seed oil (PSO), a by-product of pomegranate juice production, has a long-standing role in traditional medicine, yet is mechanistic and pharmaceutical potential for skin applications remains unexplored. This work explored the bioactivity of PSO in human keratinocytes (HaCaT) and dermal fibroblasts (HDFa) through evaluation of its impact upon cellular metabolism, anti-inflammatory properties and wound healing capacity. The data obtained showed that PSO exhibited no cytotoxic effects up to 500 µg/mL and significantly suppressed pro-inflammatory cytokines (IL-6, IL-8, TNF-α) under LPS-induced inflammatory conditions. Furthermore, wound closure in fibroblasts was accelerated by approximately 20% in a 24-h period. Although antioxidant activity was limited under induced oxidative stress, PSO exerted mild protective effects under basal conditions and fatty acid profiling revealed a PUFA-rich composition, including dihomolinolenic and punicic acids, potentially linked to the observed impact upon cellular metabolism. Overall, these findings offer novel insights into PSO's dual functionality in skin regeneration and immune modulation, supporting its application as a bioactive ingredient for advanced topical therapies.
期刊介绍:
Inflammopharmacology is the official publication of the Gastrointestinal Section of the International Union of Basic and Clinical Pharmacology (IUPHAR) and the Hungarian Experimental and Clinical Pharmacology Society (HECPS). Inflammopharmacology publishes papers on all aspects of inflammation and its pharmacological control emphasizing comparisons of (a) different inflammatory states, and (b) the actions, therapeutic efficacy and safety of drugs employed in the treatment of inflammatory conditions. The comparative aspects of the types of inflammatory conditions include gastrointestinal disease (e.g. ulcerative colitis, Crohn''s disease), parasitic diseases, toxicological manifestations of the effects of drugs and environmental agents, arthritic conditions, and inflammatory effects of injury or aging on skeletal muscle. The journal has seven main interest areas:
-Drug-Disease Interactions - Conditional Pharmacology - i.e. where the condition (disease or stress state) influences the therapeutic response and side (adverse) effects from anti-inflammatory drugs. Mechanisms of drug-disease and drug disease interactions and the role of different stress states
-Rheumatology - particular emphasis on methods of measurement of clinical response effects of new agents, adverse effects from anti-rheumatic drugs
-Gastroenterology - with particular emphasis on animal and human models, mechanisms of mucosal inflammation and ulceration and effects of novel and established anti-ulcer, anti-inflammatory agents, or antiparasitic agents
-Neuro-Inflammation and Pain - model systems, pharmacology of new analgesic agents and mechanisms of neuro-inflammation and pain
-Novel drugs, natural products and nutraceuticals - and their effects on inflammatory processes, especially where there are indications of novel modes action compared with conventional drugs e.g. NSAIDs
-Muscle-immune interactions during inflammation [...]