Yansheng Liu, Yukun Sun, Guofu Wang, Xiaobo Jia, Jin Zhou, Hongqi Li, Hongli Li, Haixin Chan, Kang Li, Zhicheng Jin, Zhaoxu Li
{"title":"利用银纳米线膜SERS底物检测血清中PFAS和纳米塑料，具有良好的可重复使用性和灵敏度。","authors":"Yansheng Liu, Yukun Sun, Guofu Wang, Xiaobo Jia, Jin Zhou, Hongqi Li, Hongli Li, Haixin Chan, Kang Li, Zhicheng Jin, Zhaoxu Li","doi":"10.1007/s00604-025-07585-0","DOIUrl":null,"url":null,"abstract":"<div><p> A novel surface-enhanced Raman spectroscopy (SERS)-based approach is introduced for the highly sensitive detection of perfluoroalkyl and polyfluoroalkyl substances (PFAS), polytetrafluoroethylene (PTFE), and polystyrene (PS) nanoplastics in serum samples using silver nanowire mesh (AgNWM) as SERS substrates. The surface-enhanced Raman scattering performance of AgNWM substrates was optimized using rhodamine 6G as a probe molecule, achieving a limit of detection (LOD) of 10<sup>−9</sup> M. Serum samples were subjected to pre-treatment steps, including protein removal, sample enrichment, and concentration, enabling quantitative detection of multiple PFAS species at as low as 10<sup>−8</sup> M. PTFE and PS nanoplastics were also successfully identified at a concentration of 100 ng·mL<sup>−1</sup>. Furthermore, the AgNWM substrates exhibited significant stability and could be reused up to 10 times for plastic detection without substantial loss of sensitivity. This highly sensitive SERS platform provides an innovative and effective methodology for the detection of PFAS, PTFE, and PS nanoplastics in complex biological matrices, with considerable potential for clinical and environmental analyses.</p><h3>Graphical Abstract</h3>\n<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 11","pages":""},"PeriodicalIF":5.3000,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Detection of PFAS and nanoplastics in serum by using Ag nanowires film SERS substrate with good reusability and sensitivity\",\"authors\":\"Yansheng Liu, Yukun Sun, Guofu Wang, Xiaobo Jia, Jin Zhou, Hongqi Li, Hongli Li, Haixin Chan, Kang Li, Zhicheng Jin, Zhaoxu Li\",\"doi\":\"10.1007/s00604-025-07585-0\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p> A novel surface-enhanced Raman spectroscopy (SERS)-based approach is introduced for the highly sensitive detection of perfluoroalkyl and polyfluoroalkyl substances (PFAS), polytetrafluoroethylene (PTFE), and polystyrene (PS) nanoplastics in serum samples using silver nanowire mesh (AgNWM) as SERS substrates. The surface-enhanced Raman scattering performance of AgNWM substrates was optimized using rhodamine 6G as a probe molecule, achieving a limit of detection (LOD) of 10<sup>−9</sup> M. Serum samples were subjected to pre-treatment steps, including protein removal, sample enrichment, and concentration, enabling quantitative detection of multiple PFAS species at as low as 10<sup>−8</sup> M. PTFE and PS nanoplastics were also successfully identified at a concentration of 100 ng·mL<sup>−1</sup>. Furthermore, the AgNWM substrates exhibited significant stability and could be reused up to 10 times for plastic detection without substantial loss of sensitivity. This highly sensitive SERS platform provides an innovative and effective methodology for the detection of PFAS, PTFE, and PS nanoplastics in complex biological matrices, with considerable potential for clinical and environmental analyses.</p><h3>Graphical Abstract</h3>\\n<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>\",\"PeriodicalId\":705,\"journal\":{\"name\":\"Microchimica Acta\",\"volume\":\"192 11\",\"pages\":\"\"},\"PeriodicalIF\":5.3000,\"publicationDate\":\"2025-10-08\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Microchimica Acta\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s00604-025-07585-0\",\"RegionNum\":2,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, ANALYTICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Microchimica Acta","FirstCategoryId":"92","ListUrlMain":"https://link.springer.com/article/10.1007/s00604-025-07585-0","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
Detection of PFAS and nanoplastics in serum by using Ag nanowires film SERS substrate with good reusability and sensitivity
A novel surface-enhanced Raman spectroscopy (SERS)-based approach is introduced for the highly sensitive detection of perfluoroalkyl and polyfluoroalkyl substances (PFAS), polytetrafluoroethylene (PTFE), and polystyrene (PS) nanoplastics in serum samples using silver nanowire mesh (AgNWM) as SERS substrates. The surface-enhanced Raman scattering performance of AgNWM substrates was optimized using rhodamine 6G as a probe molecule, achieving a limit of detection (LOD) of 10−9 M. Serum samples were subjected to pre-treatment steps, including protein removal, sample enrichment, and concentration, enabling quantitative detection of multiple PFAS species at as low as 10−8 M. PTFE and PS nanoplastics were also successfully identified at a concentration of 100 ng·mL−1. Furthermore, the AgNWM substrates exhibited significant stability and could be reused up to 10 times for plastic detection without substantial loss of sensitivity. This highly sensitive SERS platform provides an innovative and effective methodology for the detection of PFAS, PTFE, and PS nanoplastics in complex biological matrices, with considerable potential for clinical and environmental analyses.
期刊介绍：
As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.
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