β-羟基色氨酸区域异构体的生物催化合成。

IF 2.7 3区化学 Q1 CHEMISTRY, ORGANIC

Organic & Biomolecular Chemistry Pub Date : 2025-10-09 DOI:10.1039/d5ob01304f

Samantha K Bruffy, Hunter Samuel, Holly A Weilbaker, Andrew R Buller

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引用次数: 0

摘要

我们报道了一种利用l -苏氨酸转醛醇酶获得新型β-羟基l -色氨酸区域异构体的生物催化策略。我们发现经典的苏氨酸醛缩酶不能进行这种转化，突出了转醛缩酶的独特反应性。通过耦合周转和副产物的去除，实现了高达90%的高分离收率。该反应使多酶级联合成l -色氨酸的各种非规范类似物。

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Biocatalytic synthesis of β-hydroxy tryptophan regioisomers.

We report a biocatalytic strategy to access novel β-hydroxy L-tryptophan regioisomers using an L-threonine transaldolase. We show that classical threonine aldolases cannot perform this transformation, highlighting the unique reactivity of transaldolases. By coupling turnover to byproduct removal, high isolated yields of up to 90% were achieved. This reaction enables a multi-enzyme cascade to synthesize diverse non-canonical analogs of L-tryptophan.

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来源期刊

Organic & Biomolecular Chemistry 化学-有机化学

CiteScore

5.50

自引率

9.40%

发文量

1056

审稿时长

1.3 months

期刊介绍： Organic & Biomolecular Chemistry is an international journal using integrated research in chemistry-organic chemistry. Founded in 2003 by the Royal Society of Chemistry, the journal is published in Semimonthly issues and has been indexed by SCIE, a leading international database. The journal focuses on the key research and cutting-edge progress in the field of chemistry-organic chemistry, publishes and reports the research results in this field in a timely manner, and is committed to becoming a window and platform for rapid academic exchanges among peers in this field. The journal's impact factor in 2023 is 2.9, and its CiteScore is 5.5.