Xuanxiu Da, Bolu Sun, Zixia Wang, Yuqiong Wu, Huajie Li, Miao Zhou, Ying Lv, Xinlan Wang, Haiying He, Jiali Kang, Chenyv Qin, Xiangdong Wang, Lin Yang, Xiuying Pu, Hai Wang
{"title":"通过血红蛋白β-亚基检测筛查早期地中海贫血:便携式PDA-PoPD-MWCNT电化学免疫传感器。","authors":"Xuanxiu Da, Bolu Sun, Zixia Wang, Yuqiong Wu, Huajie Li, Miao Zhou, Ying Lv, Xinlan Wang, Haiying He, Jiali Kang, Chenyv Qin, Xiangdong Wang, Lin Yang, Xiuying Pu, Hai Wang","doi":"10.1039/d5ay01282a","DOIUrl":null,"url":null,"abstract":"<p><p>β-Thalassemia is a hereditary hemolytic anemia caused by an imbalance in hemoglobin (Hb) synthesis. Patients experience severe anemia, with severe cases requiring lifelong blood transfusions. The condition may also lead to iron overload-related organ damage and growth and developmental disorders, imposing significant health and economic burden. The β-subunit of hemoglobin (HB-β) serves as a key biomarker closely associated with the onset and progression of β-thalassemia. Testing for HB-β is crucial for the early screening, genetic counseling, and early intervention of β-thalassemia. Based on this, an electrochemical immunosensor comprising polydopamine/poly(<i>o</i>-phenylenediamine)/multi-walled carbon nanotubes (PDA-P<i>o</i>PD-MWCNTs) was developed to detect the HB-β antigen. With abundant hydrophilic functional groups and amino and hydroxyl groups on the composites, the HB-β antibody (Ab) can be directly allowed to associate with dopamine (DA) molecules on the PDA-P<i>o</i>PD-MWCNT electrode under mild conditions. It caused HB-β-Ab to be immobilized on the electrode surface to obtain HB-β-Ab/PDA-P<i>o</i>PD-MWCNTs/GCE. This method utilizes the specific binding between the HB-β antigen and HB-β antibody (HB-β-Ab) to achieve highly sensitive and specific detection of HB-β. It offers a convenient, efficient, and ultrasensitive diagnostic approach for early screening and intervention of β-thalassemia based on hemoglobin β subunit detection. Under the optimized experimental conditions, the sensor exhibits an excellent linear relationship in the concentration range of 1.0-4.0 × 10<sup>5</sup> ng mL<sup>-1</sup>, and the detection limit is 0.3 ng mL<sup>-1</sup>. This study presents an efficient, rapid, and low-cost diagnostic approach for early β-thalassemia screening, which is crucial for precise early detection and intervention in high-prevalence regions.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.6000,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Early thalassemia screening <i>via</i> hemoglobin β-subunit detection: a portable PDA-P<i>o</i>PD-MWCNT electrochemical immunosensor.\",\"authors\":\"Xuanxiu Da, Bolu Sun, Zixia Wang, Yuqiong Wu, Huajie Li, Miao Zhou, Ying Lv, Xinlan Wang, Haiying He, Jiali Kang, Chenyv Qin, Xiangdong Wang, Lin Yang, Xiuying Pu, Hai Wang\",\"doi\":\"10.1039/d5ay01282a\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>β-Thalassemia is a hereditary hemolytic anemia caused by an imbalance in hemoglobin (Hb) synthesis. Patients experience severe anemia, with severe cases requiring lifelong blood transfusions. The condition may also lead to iron overload-related organ damage and growth and developmental disorders, imposing significant health and economic burden. The β-subunit of hemoglobin (HB-β) serves as a key biomarker closely associated with the onset and progression of β-thalassemia. Testing for HB-β is crucial for the early screening, genetic counseling, and early intervention of β-thalassemia. Based on this, an electrochemical immunosensor comprising polydopamine/poly(<i>o</i>-phenylenediamine)/multi-walled carbon nanotubes (PDA-P<i>o</i>PD-MWCNTs) was developed to detect the HB-β antigen. With abundant hydrophilic functional groups and amino and hydroxyl groups on the composites, the HB-β antibody (Ab) can be directly allowed to associate with dopamine (DA) molecules on the PDA-P<i>o</i>PD-MWCNT electrode under mild conditions. It caused HB-β-Ab to be immobilized on the electrode surface to obtain HB-β-Ab/PDA-P<i>o</i>PD-MWCNTs/GCE. This method utilizes the specific binding between the HB-β antigen and HB-β antibody (HB-β-Ab) to achieve highly sensitive and specific detection of HB-β. It offers a convenient, efficient, and ultrasensitive diagnostic approach for early screening and intervention of β-thalassemia based on hemoglobin β subunit detection. Under the optimized experimental conditions, the sensor exhibits an excellent linear relationship in the concentration range of 1.0-4.0 × 10<sup>5</sup> ng mL<sup>-1</sup>, and the detection limit is 0.3 ng mL<sup>-1</sup>. This study presents an efficient, rapid, and low-cost diagnostic approach for early β-thalassemia screening, which is crucial for precise early detection and intervention in high-prevalence regions.</p>\",\"PeriodicalId\":64,\"journal\":{\"name\":\"Analytical Methods\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":2.6000,\"publicationDate\":\"2025-10-09\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Analytical Methods\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://doi.org/10.1039/d5ay01282a\",\"RegionNum\":3,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"CHEMISTRY, ANALYTICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Analytical Methods","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1039/d5ay01282a","RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
引用次数: 0
摘要
β-地中海贫血是一种遗传性溶血性贫血,由血红蛋白(Hb)合成失衡引起。患者会出现严重的贫血,严重者需要终生输血。这种情况还可能导致与铁超载有关的器官损伤和生长发育障碍,造成重大的健康和经济负担。血红蛋白的β亚基(HB-β)是与β-地中海贫血的发生和进展密切相关的关键生物标志物。HB-β检测对于β-地中海贫血的早期筛查、遗传咨询和早期干预至关重要。在此基础上,开发了一种由聚多巴胺/聚邻苯二胺/多壁碳纳米管(PDA-PoPD-MWCNTs)组成的电化学免疫传感器,用于检测HB-β抗原。由于复合材料具有丰富的亲水性官能团以及氨基和羟基,HB-β抗体(Ab)可以在温和的条件下直接与PDA-PoPD-MWCNT电极上的多巴胺(DA)分子结合。将HB-β-Ab固定在电极表面,得到HB-β-Ab/PDA-PoPD-MWCNTs/GCE。该方法利用HB-β抗原与HB-β抗体(HB-β- ab)的特异性结合,实现对HB-β的高灵敏度和特异性检测。它为基于血红蛋白β亚基检测的β-地中海贫血的早期筛查和干预提供了一种方便、高效、超灵敏的诊断方法。在优化的实验条件下,传感器在1.0 ~ 4.0 × 105 ng mL-1的浓度范围内呈良好的线性关系,检测限为0.3 ng mL-1。本研究提出了一种高效、快速、低成本的β-地中海贫血早期筛查诊断方法,这对于高患病率地区的精确早期发现和干预至关重要。
Early thalassemia screening via hemoglobin β-subunit detection: a portable PDA-PoPD-MWCNT electrochemical immunosensor.
β-Thalassemia is a hereditary hemolytic anemia caused by an imbalance in hemoglobin (Hb) synthesis. Patients experience severe anemia, with severe cases requiring lifelong blood transfusions. The condition may also lead to iron overload-related organ damage and growth and developmental disorders, imposing significant health and economic burden. The β-subunit of hemoglobin (HB-β) serves as a key biomarker closely associated with the onset and progression of β-thalassemia. Testing for HB-β is crucial for the early screening, genetic counseling, and early intervention of β-thalassemia. Based on this, an electrochemical immunosensor comprising polydopamine/poly(o-phenylenediamine)/multi-walled carbon nanotubes (PDA-PoPD-MWCNTs) was developed to detect the HB-β antigen. With abundant hydrophilic functional groups and amino and hydroxyl groups on the composites, the HB-β antibody (Ab) can be directly allowed to associate with dopamine (DA) molecules on the PDA-PoPD-MWCNT electrode under mild conditions. It caused HB-β-Ab to be immobilized on the electrode surface to obtain HB-β-Ab/PDA-PoPD-MWCNTs/GCE. This method utilizes the specific binding between the HB-β antigen and HB-β antibody (HB-β-Ab) to achieve highly sensitive and specific detection of HB-β. It offers a convenient, efficient, and ultrasensitive diagnostic approach for early screening and intervention of β-thalassemia based on hemoglobin β subunit detection. Under the optimized experimental conditions, the sensor exhibits an excellent linear relationship in the concentration range of 1.0-4.0 × 105 ng mL-1, and the detection limit is 0.3 ng mL-1. This study presents an efficient, rapid, and low-cost diagnostic approach for early β-thalassemia screening, which is crucial for precise early detection and intervention in high-prevalence regions.