用于选择性荧光检测神经肌肉阻滞剂罗库溴铵的超分子材料。

IF 4.7 Q2 MATERIALS SCIENCE, BIOMATERIALS

ACS Applied Bio Materials Pub Date : 2025-10-09 DOI:10.1021/acsabm.5c01490

Bindurani Padhan, Prabhat K Singh

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引用次数: 0

摘要

罗库溴铵（Rocuronium Bromide, RcBr）是一种广泛应用于麻醉的神经肌肉阻滞剂，其监测在临床上具有重要意义，但在分析上还未得到充分的探讨。我们报道了一种基于荧光的RcBr传感器，该传感器使用γ-硫酸环糊精（γ-SCD）模板化的硫黄素- t （ThT）聚集体。这种竞争性置换试验利用罗库溴铵对γ-SCD的高结合亲和力，从超分子聚集体中置换硫黄素- t，从而产生荧光关闭反应。该传感器具有亚微摩尔灵敏度（LOD ~ 0.2±0.02 μM）、快速响应(

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A Supramolecular Material for Selective Fluorescence-Based Detection of the Neuromuscular Blocker Rocuronium Bromide.

Monitoring Rocuronium Bromide (RcBr), a neuromuscular blocker widely used in anesthesia, is clinically important yet analytically underexplored. We report a fluorescence-based sensor for RcBr using Thioflavin-T (ThT) aggregates templated by γ-sulfated cyclodextrin (γ-SCD). This competitive displacement assay exploits the high binding affinity of Rocuronium for γ-SCD to displace Thioflavin-T from supramolecular aggregates, resulting in a fluorescence turn-off response. The sensor exhibits submicromolar sensitivity (LOD ∼ 0.2 ± 0.02 μM), rapid response (<1 min), and high selectivity over biological interferents. Its functionality in diluted human serum demonstrates potential for possible clinical applications. This work presents a novel optical approach for RcBr sensing that may support future developments in neuromuscular blockade monitoring.

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来源期刊

ACS Applied Bio Materials Chemistry-Chemistry (all)

CiteScore

9.40

自引率

2.10%

发文量

464

期刊介绍： ACS Applied Bio Materials is an interdisciplinary journal publishing original research covering all aspects of biomaterials and biointerfaces including and beyond the traditional biosensing, biomedical and therapeutic applications. The journal is devoted to reports of new and original experimental and theoretical research of an applied nature that integrates knowledge in the areas of materials, engineering, physics, bioscience, and chemistry into important bio applications. The journal is specifically interested in work that addresses the relationship between structure and function and assesses the stability and degradation of materials under relevant environmental and biological conditions.