印度VINS多价抗蛇毒血清中和孟加拉罗素毒蛇蛇毒的生化和生物学特征。

IF 3 Q2 TOXICOLOGY
Journal of Toxicology Pub Date : 2025-09-29 eCollection Date: 2025-01-01 DOI:10.1155/jt/5464388
Rubait Hasan, Hasanuzzaman Manik, Md Ataur Rahman, Jamiatul Husna Shathi, Md Tariqul Islam, Mohammad Shahangir Biswas, Kazi Md Faisal Hoque
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引用次数: 0

摘要

罗素蝰蛇(Daboia russelii)最近成为孟加拉国人类生命的重大威胁。鉴于其在南亚的广泛分布,毒液的特征和致命性可能因地区不同而异,具有不同的毒理学特性。因此,我们通过SDS-PAGE分析、反相高效液相色谱分析,以及磷脂酶A2 (PLA2)、致水肿、溶血、出血性和凝血活性、组织病理学和血液生化的评估,研究了孟加拉国罗素蝰蛇毒液(BRVV)的特征。我们还研究了印度VINS Bio Products Ltd.的多价抗蛇毒血清(VPAV)对BRVV的中和作用。BRVV的RP-HPLC分析显示15个峰,SDS-PAGE显示15-70 kDa范围内的高强度蛋白带。小鼠的中位致死剂量(LD50)为0.33 mg/kg腹腔注射,毒液暴露导致神经毒性症状,如肢体瘫痪、呼吸困难和行动迟缓。BRVV具有较强的PLA2、促凝、出血性、间接溶血和致水肿活性,但直接溶血活性较差。小鼠血清中谷丙转氨酶(ALT)、碱性磷酸酶(ALP)、天冬氨酸转氨酶(AST)、胆固醇、总蛋白、尿酸、血尿素氮(BUN)、肌酐水平显著升高,提示器官损伤。组织病理学检查显示细胞空泡化、充血、出血、炎症浸润和坏死,证实血清生化异常。中和研究显示,VPAV对BRVV的作用有限,这表明抗蛇毒血清中存在未完全中和的毒液蛋白。总之,这些发现表明,罗素蝰蛇在孟加拉国是一种医学上重要的毒蛇,VPAV在减少毒液的毒性作用方面只是部分有效。因此,区域特异性毒液必须考虑在抗蛇毒血清开发更有效地治疗中毒的情况下。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Biochemical and Biological Profiles of Bangladeshi Russell's Viper Snake Venom and Neutralizing Efficacy by Indian VINS Polyvalent Antivenom.

Biochemical and Biological Profiles of Bangladeshi Russell's Viper Snake Venom and Neutralizing Efficacy by Indian VINS Polyvalent Antivenom.

Biochemical and Biological Profiles of Bangladeshi Russell's Viper Snake Venom and Neutralizing Efficacy by Indian VINS Polyvalent Antivenom.

Biochemical and Biological Profiles of Bangladeshi Russell's Viper Snake Venom and Neutralizing Efficacy by Indian VINS Polyvalent Antivenom.

The Russell's viper (Daboia russelii) has recently become a significant threat to human life in Bangladesh. Given its wide distribution across South Asia, the venom characteristics and lethality can vary by region with different toxicological properties. Hence, we investigated the characteristics of Bangladeshi Russell's viper venom (BRVV) through SDS-PAGE profiling, reverse-phase HPLC analysis, along with assessments of phospholipase A2 (PLA2), edema-inducing, hemolytic, hemorrhagic, and coagulant activities, histopathology, and blood biochemistry, following established protocols. We also studied the neutralization efficacy of polyvalent antivenom from VINS Bio Products Ltd., India (VPAV) against BRVV. RP-HPLC analysis of BRVV displayed 15 peaks, and SDS-PAGE showed high-intensity protein bands within the 15-70 kDa range. The median lethal dose (LD50) for mice was found to be 0.33 mg/kg intraperitoneally (i.p.), and venom exposure resulted in neurotoxic symptoms such as limb paralysis, respiratory difficulties, and sluggishness. BRVV exhibited strong PLA2, procoagulant, hemorrhagic, indirect hemolytic, and edema-inducing activities but poor direct hemolytic activity. Venom administration also significantly increased levels of alanine aminotransferase (ALT), alkaline phosphatase (ALP), aspartate aminotransferase (AST), cholesterol, total protein, uric acid, blood urea nitrogen (BUN), and creatinine in mouse serum, indicating organ damage. Histopathological examination revealed cell vacuolization, congestion, hemorrhage, inflammatory infiltrations, and necrosis in venom-exposed tissues, validating the abnormal serum biochemistry. The neutralization study revealed that VPAV had limited efficacy against BRVV, suggesting the presence of venom proteins not fully neutralized by the antivenom. Altogether, these findings suggest that the Russell's viper is a medically significant venomous snake in Bangladesh, and VPAV is only partially effective in reducing the venom's toxic effects. Therefore, region-specific venoms must be considered in antivenom development for more effective treatment in envenomation cases.

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来源期刊
Journal of Toxicology
Journal of Toxicology TOXICOLOGY-
CiteScore
5.50
自引率
3.40%
发文量
0
审稿时长
10 weeks
期刊介绍: Journal of Toxicology is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies in all areas of toxicological sciences. The journal will consider articles looking at the structure, function, and mechanism of agents that are toxic to humans and/or animals, as well as toxicological medicine, risk assessment, safety evaluation, and environmental health.
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