Nathaniel Ogunkunle, Monya Simpson, Felix Samuel, Xianyan Kuang, Ernst Cebert, Gamal AbdelRahim, Judith Boateng
{"title":"添加工业大麻对安格斯牛反刍行为、血浆抗氧化酶和应激生物标志物的影响","authors":"Nathaniel Ogunkunle, Monya Simpson, Felix Samuel, Xianyan Kuang, Ernst Cebert, Gamal AbdelRahim, Judith Boateng","doi":"10.1111/jpn.70014","DOIUrl":null,"url":null,"abstract":"<p><p>Oxidative stress is known to affect the behaviour, performance and well-being of cattle. The objective of this study was to determine the effect of industrial hemp (IH) supplementation on rumination behaviour, stress biomarkers and antioxidant enzymes in Angus cattle. A total of 20 black Angus heifers were completely randomized into either control (CON) (receiving commercial concentrates) or IH (150 g of IH/kg of commercial concentrates) in a trial that lasted for 35 days with IH supplementation for 21 days and 14 days post-supplementation. Rumination behaviour (rumination time, ruminal pH, temperature and water intake) was recorded with smaXtec bolus, plasma antioxidants (total antioxidant activity [TAA], superoxide dismutase [SOD], glutathione peroxidase [Gpx], catalase [CAT], nitric oxide [NO], malondialdehyde [MDA]) and stress biomarkers (cortisol and heat shock proteins) were analyzed. Results showed that IH supplementation decreased water intake with an average consumption of 51.39 L/day in the IH group and 55.09 L/day in the CON. No significant (p = 0.67) difference was observed in the ruminal pH and rumination time (p = 0.58) of the animals. The TAA increased from 7.26 to 17.95 U/mL in the IH group during supplementation but decreased to 11.48 U/mL post-supplementation. GPx was significantly (p = 0.0023) higher in IH (46.47 mmol/L) than in CON (44.01 mmol/L) group. Similar results were observed with NO. MDA was significantly (p < 0.05) higher in the CON compared to the IH group. Cortisol was significantly (p = 0.03) lower in the IH group (97.54 ng/mL) than CON (122.23 ng/mL). No significant (p < 0.05) difference was observed for heat shock proteins 70 and 72. Conclusively, IH improved rumination behaviour in cattle by maintaining optimum rumen pH. Furthermore, IH increased the antioxidant status as well as the reduction of oxidative stress biomarkers, suggesting that IH supplementation could improve the health and welfare of Angus cattle.</p>","PeriodicalId":14942,"journal":{"name":"Journal of Animal Physiology and Animal Nutrition","volume":" ","pages":""},"PeriodicalIF":2.4000,"publicationDate":"2025-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Effect of Industrial Hemp (Cannabis sativa) Supplementation on Rumination Behaviour, Plasma Antioxidant Enzymes and Stress Biomarkers in Angus Cattle.\",\"authors\":\"Nathaniel Ogunkunle, Monya Simpson, Felix Samuel, Xianyan Kuang, Ernst Cebert, Gamal AbdelRahim, Judith Boateng\",\"doi\":\"10.1111/jpn.70014\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Oxidative stress is known to affect the behaviour, performance and well-being of cattle. The objective of this study was to determine the effect of industrial hemp (IH) supplementation on rumination behaviour, stress biomarkers and antioxidant enzymes in Angus cattle. A total of 20 black Angus heifers were completely randomized into either control (CON) (receiving commercial concentrates) or IH (150 g of IH/kg of commercial concentrates) in a trial that lasted for 35 days with IH supplementation for 21 days and 14 days post-supplementation. Rumination behaviour (rumination time, ruminal pH, temperature and water intake) was recorded with smaXtec bolus, plasma antioxidants (total antioxidant activity [TAA], superoxide dismutase [SOD], glutathione peroxidase [Gpx], catalase [CAT], nitric oxide [NO], malondialdehyde [MDA]) and stress biomarkers (cortisol and heat shock proteins) were analyzed. Results showed that IH supplementation decreased water intake with an average consumption of 51.39 L/day in the IH group and 55.09 L/day in the CON. No significant (p = 0.67) difference was observed in the ruminal pH and rumination time (p = 0.58) of the animals. The TAA increased from 7.26 to 17.95 U/mL in the IH group during supplementation but decreased to 11.48 U/mL post-supplementation. GPx was significantly (p = 0.0023) higher in IH (46.47 mmol/L) than in CON (44.01 mmol/L) group. Similar results were observed with NO. MDA was significantly (p < 0.05) higher in the CON compared to the IH group. Cortisol was significantly (p = 0.03) lower in the IH group (97.54 ng/mL) than CON (122.23 ng/mL). No significant (p < 0.05) difference was observed for heat shock proteins 70 and 72. Conclusively, IH improved rumination behaviour in cattle by maintaining optimum rumen pH. 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Effect of Industrial Hemp (Cannabis sativa) Supplementation on Rumination Behaviour, Plasma Antioxidant Enzymes and Stress Biomarkers in Angus Cattle.
Oxidative stress is known to affect the behaviour, performance and well-being of cattle. The objective of this study was to determine the effect of industrial hemp (IH) supplementation on rumination behaviour, stress biomarkers and antioxidant enzymes in Angus cattle. A total of 20 black Angus heifers were completely randomized into either control (CON) (receiving commercial concentrates) or IH (150 g of IH/kg of commercial concentrates) in a trial that lasted for 35 days with IH supplementation for 21 days and 14 days post-supplementation. Rumination behaviour (rumination time, ruminal pH, temperature and water intake) was recorded with smaXtec bolus, plasma antioxidants (total antioxidant activity [TAA], superoxide dismutase [SOD], glutathione peroxidase [Gpx], catalase [CAT], nitric oxide [NO], malondialdehyde [MDA]) and stress biomarkers (cortisol and heat shock proteins) were analyzed. Results showed that IH supplementation decreased water intake with an average consumption of 51.39 L/day in the IH group and 55.09 L/day in the CON. No significant (p = 0.67) difference was observed in the ruminal pH and rumination time (p = 0.58) of the animals. The TAA increased from 7.26 to 17.95 U/mL in the IH group during supplementation but decreased to 11.48 U/mL post-supplementation. GPx was significantly (p = 0.0023) higher in IH (46.47 mmol/L) than in CON (44.01 mmol/L) group. Similar results were observed with NO. MDA was significantly (p < 0.05) higher in the CON compared to the IH group. Cortisol was significantly (p = 0.03) lower in the IH group (97.54 ng/mL) than CON (122.23 ng/mL). No significant (p < 0.05) difference was observed for heat shock proteins 70 and 72. Conclusively, IH improved rumination behaviour in cattle by maintaining optimum rumen pH. Furthermore, IH increased the antioxidant status as well as the reduction of oxidative stress biomarkers, suggesting that IH supplementation could improve the health and welfare of Angus cattle.
期刊介绍：
As an international forum for hypothesis-driven scientific research, the Journal of Animal Physiology and Animal Nutrition publishes original papers in the fields of animal physiology, biochemistry and physiology of nutrition, animal nutrition, feed technology and preservation (only when related to animal nutrition). Well-conducted scientific work that meets the technical and ethical standards is considered only on the basis of scientific rigor.
Research on farm and companion animals is preferred. Comparative work on exotic species is welcome too. Pharmacological or toxicological experiments with a direct reference to nutrition are also considered. Manuscripts on fish and other aquatic non-mammals with topics on growth or nutrition will not be accepted. Manuscripts may be rejected on the grounds that the subject is too specialized or that the contribution they make to animal physiology and nutrition is insufficient.
In addition, reviews on topics of current interest within the scope of the journal are welcome. Authors are advised to send an outline to the Editorial Office for approval prior to submission.
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