{"title":"利用多组学方法揭示桑果实脱落机制。","authors":"Jiahu Yang, Sha Li, Zhennan Li, Yahui Xuan, Jiamei He, Mingju Ruan, Yuming Feng, Zhenyang Tao, Xiaoru Kang, Zhengang Li","doi":"10.3389/fpls.2025.1605312","DOIUrl":null,"url":null,"abstract":"<p><strong>Introduction: </strong><i>Morus laevigata</i> (long-fruited mulberry) is rich in active components and possesses significant nutritive value. The fruitlet stage represents a critical period for fruit abscission, and elucidating the underlying biological mechanisms of this process can provide a theoretical foundation for breeding more stable and abscission resistant cultivars.</p><p><strong>Methods: </strong>Fruit peduncles at the fruit set stage (April to May) were selected as experimental materials, including both abscising and non-abscising fruits. Morphological analysis of the peduncle abscission zone was conducted to examine structural differences. Additionally, transcriptomic and metabolomic analyses were performed to investigate gene expression and metabolite changes associated with fruit abscission.</p><p><strong>Results: </strong>Morphological analysis of the peduncle abscission zone in abscising fruits revealed enlarged intercellular spaces and disorganized cell arrangements. Transcriptomic and metabolomic analyses indicated that genes and metabolites related to fruit abscission were primarily involved in plant hormone signal transduction, and starch and sucrose metabolism pathways. Auxin and abscisic acid were identified as key regulators, modulating the expression of cell wall-degrading enzymes, which facilitated cell wall loosening and degradation, ultimately leading to fruit abscission. Furthermore, alterations in energy metabolism were found to play a pivotal role in this process.</p><p><strong>Conclusion: </strong>These findings contribute to a deeper understanding of the physiological mechanisms underlying fruit development and abscission, offering valuable insights into mulberry breeding and the sustainable advancement of modern agriculture.</p>","PeriodicalId":12632,"journal":{"name":"Frontiers in Plant Science","volume":"16 ","pages":"1605312"},"PeriodicalIF":4.1000,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12498018/pdf/","citationCount":"0","resultStr":"{\"title\":\"Unraveling the mechanisms of fruit abscission in <i>Morus laevigata</i> through multi-omics approaches.\",\"authors\":\"Jiahu Yang, Sha Li, Zhennan Li, Yahui Xuan, Jiamei He, Mingju Ruan, Yuming Feng, Zhenyang Tao, Xiaoru Kang, Zhengang Li\",\"doi\":\"10.3389/fpls.2025.1605312\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Introduction: </strong><i>Morus laevigata</i> (long-fruited mulberry) is rich in active components and possesses significant nutritive value. The fruitlet stage represents a critical period for fruit abscission, and elucidating the underlying biological mechanisms of this process can provide a theoretical foundation for breeding more stable and abscission resistant cultivars.</p><p><strong>Methods: </strong>Fruit peduncles at the fruit set stage (April to May) were selected as experimental materials, including both abscising and non-abscising fruits. Morphological analysis of the peduncle abscission zone was conducted to examine structural differences. Additionally, transcriptomic and metabolomic analyses were performed to investigate gene expression and metabolite changes associated with fruit abscission.</p><p><strong>Results: </strong>Morphological analysis of the peduncle abscission zone in abscising fruits revealed enlarged intercellular spaces and disorganized cell arrangements. Transcriptomic and metabolomic analyses indicated that genes and metabolites related to fruit abscission were primarily involved in plant hormone signal transduction, and starch and sucrose metabolism pathways. Auxin and abscisic acid were identified as key regulators, modulating the expression of cell wall-degrading enzymes, which facilitated cell wall loosening and degradation, ultimately leading to fruit abscission. Furthermore, alterations in energy metabolism were found to play a pivotal role in this process.</p><p><strong>Conclusion: </strong>These findings contribute to a deeper understanding of the physiological mechanisms underlying fruit development and abscission, offering valuable insights into mulberry breeding and the sustainable advancement of modern agriculture.</p>\",\"PeriodicalId\":12632,\"journal\":{\"name\":\"Frontiers in Plant Science\",\"volume\":\"16 \",\"pages\":\"1605312\"},\"PeriodicalIF\":4.1000,\"publicationDate\":\"2025-09-22\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12498018/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Frontiers in Plant Science\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.3389/fpls.2025.1605312\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q1\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Frontiers in Plant Science","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.3389/fpls.2025.1605312","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/1 0:00:00","PubModel":"eCollection","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
Unraveling the mechanisms of fruit abscission in Morus laevigata through multi-omics approaches.
Introduction: Morus laevigata (long-fruited mulberry) is rich in active components and possesses significant nutritive value. The fruitlet stage represents a critical period for fruit abscission, and elucidating the underlying biological mechanisms of this process can provide a theoretical foundation for breeding more stable and abscission resistant cultivars.
Methods: Fruit peduncles at the fruit set stage (April to May) were selected as experimental materials, including both abscising and non-abscising fruits. Morphological analysis of the peduncle abscission zone was conducted to examine structural differences. Additionally, transcriptomic and metabolomic analyses were performed to investigate gene expression and metabolite changes associated with fruit abscission.
Results: Morphological analysis of the peduncle abscission zone in abscising fruits revealed enlarged intercellular spaces and disorganized cell arrangements. Transcriptomic and metabolomic analyses indicated that genes and metabolites related to fruit abscission were primarily involved in plant hormone signal transduction, and starch and sucrose metabolism pathways. Auxin and abscisic acid were identified as key regulators, modulating the expression of cell wall-degrading enzymes, which facilitated cell wall loosening and degradation, ultimately leading to fruit abscission. Furthermore, alterations in energy metabolism were found to play a pivotal role in this process.
Conclusion: These findings contribute to a deeper understanding of the physiological mechanisms underlying fruit development and abscission, offering valuable insights into mulberry breeding and the sustainable advancement of modern agriculture.
期刊介绍:
In an ever changing world, plant science is of the utmost importance for securing the future well-being of humankind. Plants provide oxygen, food, feed, fibers, and building materials. In addition, they are a diverse source of industrial and pharmaceutical chemicals. Plants are centrally important to the health of ecosystems, and their understanding is critical for learning how to manage and maintain a sustainable biosphere. Plant science is extremely interdisciplinary, reaching from agricultural science to paleobotany, and molecular physiology to ecology. It uses the latest developments in computer science, optics, molecular biology and genomics to address challenges in model systems, agricultural crops, and ecosystems. Plant science research inquires into the form, function, development, diversity, reproduction, evolution and uses of both higher and lower plants and their interactions with other organisms throughout the biosphere. Frontiers in Plant Science welcomes outstanding contributions in any field of plant science from basic to applied research, from organismal to molecular studies, from single plant analysis to studies of populations and whole ecosystems, and from molecular to biophysical to computational approaches.
Frontiers in Plant Science publishes articles on the most outstanding discoveries across a wide research spectrum of Plant Science. The mission of Frontiers in Plant Science is to bring all relevant Plant Science areas together on a single platform.