Martina Tozzetti, Maria Raffaella Martina, Giacomo Lucchesi, Kristian Vasa, Ahtsham Ishaq, Laura Marsili, Piero Procacci, Stefano Menichetti, Gabriella Caminati
{"title":"利用合成受体功能化QCM纳米平台超灵敏检测FKBP12","authors":"Martina Tozzetti, Maria Raffaella Martina, Giacomo Lucchesi, Kristian Vasa, Ahtsham Ishaq, Laura Marsili, Piero Procacci, Stefano Menichetti, Gabriella Caminati","doi":"10.1002/adsr.202500053","DOIUrl":null,"url":null,"abstract":"<p>FKBP12, a peptidyl-prolyl isomerase implicated in cancer, neurodegenerative diseases, and post-transplant anti-rejection mechanisms, represents a critical biomarker for early diagnosis and monitoring. Here, a novel diagnostic nanoplatform is presented for the detection of FKBP12 at nanomolar to picomolar concentrations in biological fluids. The platform integrates a gold-coated Quartz Crystal Microbalance (QCM) functionalized with a synthetic receptor (GPS-SH1) and spacers within a Self-Assembled Monolayer (SAM), enabling direct and label-free detection of FKBP12 in complex biological samples. A careful strategy for the in-silico design and custom synthesis of the receptor is adopted, ensuring optimal binding affinity and additional chemical functionalities for surface chemisorption. The designed nano-architecture demonstrates exceptional sensitivity, with a detection limit in the picomolar range, and high selectivity, as confirmed by minimal interference from abundant serum proteins such as Serum Albumin and Immune Gamma Globulin. Furthermore, the SAM-functionalized sensors exhibit remarkable stability, retaining functionality for up to six months under storage conditions. This work not only advances the field of nanoscale biosensing but also provides a robust, reusable tool for FKBP12 detection, with potential applications in point-of-care diagnostics and personalized medicine. The platform's ability to operate in biologically relevant environments underscores its promise for real-world healthcare applications, including early disease diagnostics.</p>","PeriodicalId":100037,"journal":{"name":"Advanced Sensor Research","volume":"4 10","pages":""},"PeriodicalIF":3.5000,"publicationDate":"2025-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://advanced.onlinelibrary.wiley.com/doi/epdf/10.1002/adsr.202500053","citationCount":"0","resultStr":"{\"title\":\"Ultrasensitive Detection of FKBP12 Using a Synthetic Receptor-Functionalized QCM Nanoplatform\",\"authors\":\"Martina Tozzetti, Maria Raffaella Martina, Giacomo Lucchesi, Kristian Vasa, Ahtsham Ishaq, Laura Marsili, Piero Procacci, Stefano Menichetti, Gabriella Caminati\",\"doi\":\"10.1002/adsr.202500053\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>FKBP12, a peptidyl-prolyl isomerase implicated in cancer, neurodegenerative diseases, and post-transplant anti-rejection mechanisms, represents a critical biomarker for early diagnosis and monitoring. Here, a novel diagnostic nanoplatform is presented for the detection of FKBP12 at nanomolar to picomolar concentrations in biological fluids. The platform integrates a gold-coated Quartz Crystal Microbalance (QCM) functionalized with a synthetic receptor (GPS-SH1) and spacers within a Self-Assembled Monolayer (SAM), enabling direct and label-free detection of FKBP12 in complex biological samples. A careful strategy for the in-silico design and custom synthesis of the receptor is adopted, ensuring optimal binding affinity and additional chemical functionalities for surface chemisorption. The designed nano-architecture demonstrates exceptional sensitivity, with a detection limit in the picomolar range, and high selectivity, as confirmed by minimal interference from abundant serum proteins such as Serum Albumin and Immune Gamma Globulin. Furthermore, the SAM-functionalized sensors exhibit remarkable stability, retaining functionality for up to six months under storage conditions. This work not only advances the field of nanoscale biosensing but also provides a robust, reusable tool for FKBP12 detection, with potential applications in point-of-care diagnostics and personalized medicine. The platform's ability to operate in biologically relevant environments underscores its promise for real-world healthcare applications, including early disease diagnostics.</p>\",\"PeriodicalId\":100037,\"journal\":{\"name\":\"Advanced Sensor Research\",\"volume\":\"4 10\",\"pages\":\"\"},\"PeriodicalIF\":3.5000,\"publicationDate\":\"2025-08-07\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://advanced.onlinelibrary.wiley.com/doi/epdf/10.1002/adsr.202500053\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Advanced Sensor Research\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://advanced.onlinelibrary.wiley.com/doi/10.1002/adsr.202500053\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Advanced Sensor Research","FirstCategoryId":"1085","ListUrlMain":"https://advanced.onlinelibrary.wiley.com/doi/10.1002/adsr.202500053","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Ultrasensitive Detection of FKBP12 Using a Synthetic Receptor-Functionalized QCM Nanoplatform
FKBP12, a peptidyl-prolyl isomerase implicated in cancer, neurodegenerative diseases, and post-transplant anti-rejection mechanisms, represents a critical biomarker for early diagnosis and monitoring. Here, a novel diagnostic nanoplatform is presented for the detection of FKBP12 at nanomolar to picomolar concentrations in biological fluids. The platform integrates a gold-coated Quartz Crystal Microbalance (QCM) functionalized with a synthetic receptor (GPS-SH1) and spacers within a Self-Assembled Monolayer (SAM), enabling direct and label-free detection of FKBP12 in complex biological samples. A careful strategy for the in-silico design and custom synthesis of the receptor is adopted, ensuring optimal binding affinity and additional chemical functionalities for surface chemisorption. The designed nano-architecture demonstrates exceptional sensitivity, with a detection limit in the picomolar range, and high selectivity, as confirmed by minimal interference from abundant serum proteins such as Serum Albumin and Immune Gamma Globulin. Furthermore, the SAM-functionalized sensors exhibit remarkable stability, retaining functionality for up to six months under storage conditions. This work not only advances the field of nanoscale biosensing but also provides a robust, reusable tool for FKBP12 detection, with potential applications in point-of-care diagnostics and personalized medicine. The platform's ability to operate in biologically relevant environments underscores its promise for real-world healthcare applications, including early disease diagnostics.