利用合成受体功能化QCM纳米平台超灵敏检测FKBP12

IF 3.5
Martina Tozzetti, Maria Raffaella Martina, Giacomo Lucchesi, Kristian Vasa, Ahtsham Ishaq, Laura Marsili, Piero Procacci, Stefano Menichetti, Gabriella Caminati
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引用次数: 0

摘要

FKBP12是一种与癌症、神经退行性疾病和移植后抗排斥机制有关的肽酰脯氨酸异构酶,是早期诊断和监测的重要生物标志物。本文提出了一种新的诊断纳米平台,用于检测生物液体中纳摩尔至皮摩尔浓度的FKBP12。该平台在自组装单层(SAM)中集成了一个具有合成受体(GPS-SH1)和间隔器功能的镀金石英晶体微天平(QCM),可以直接和无标记地检测复杂生物样品中的FKBP12。采用了精心的硅设计和定制合成受体策略,确保了最佳的结合亲和力和表面化学吸附的附加化学功能。所设计的纳米结构具有卓越的灵敏度,检测限在皮摩尔范围内,并且具有高选择性,这是由大量血清蛋白(如血清白蛋白和免疫γ球蛋白)的最小干扰所证实的。此外,sam功能化的传感器表现出显著的稳定性,在存储条件下可保持长达六个月的功能。这项工作不仅推动了纳米级生物传感领域的发展,而且为FKBP12检测提供了一个强大的、可重复使用的工具,在即时诊断和个性化医疗中具有潜在的应用前景。该平台在生物相关环境中运行的能力凸显了其在现实世界医疗保健应用中的前景,包括早期疾病诊断。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Ultrasensitive Detection of FKBP12 Using a Synthetic Receptor-Functionalized QCM Nanoplatform

Ultrasensitive Detection of FKBP12 Using a Synthetic Receptor-Functionalized QCM Nanoplatform

FKBP12, a peptidyl-prolyl isomerase implicated in cancer, neurodegenerative diseases, and post-transplant anti-rejection mechanisms, represents a critical biomarker for early diagnosis and monitoring. Here, a novel diagnostic nanoplatform is presented for the detection of FKBP12 at nanomolar to picomolar concentrations in biological fluids. The platform integrates a gold-coated Quartz Crystal Microbalance (QCM) functionalized with a synthetic receptor (GPS-SH1) and spacers within a Self-Assembled Monolayer (SAM), enabling direct and label-free detection of FKBP12 in complex biological samples. A careful strategy for the in-silico design and custom synthesis of the receptor is adopted, ensuring optimal binding affinity and additional chemical functionalities for surface chemisorption. The designed nano-architecture demonstrates exceptional sensitivity, with a detection limit in the picomolar range, and high selectivity, as confirmed by minimal interference from abundant serum proteins such as Serum Albumin and Immune Gamma Globulin. Furthermore, the SAM-functionalized sensors exhibit remarkable stability, retaining functionality for up to six months under storage conditions. This work not only advances the field of nanoscale biosensing but also provides a robust, reusable tool for FKBP12 detection, with potential applications in point-of-care diagnostics and personalized medicine. The platform's ability to operate in biologically relevant environments underscores its promise for real-world healthcare applications, including early disease diagnostics.

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