{"title":"水稻伪黄化蛋白基因的编辑促进叶绿体发育","authors":"Heebak Choi, Tae Gyu Yi, Yun-Shil Gho, Ki-Hong Jung, Sun-Hwa Ha","doi":"10.1093/plcell/koaf235","DOIUrl":null,"url":null,"abstract":"The rice (Oryza sativa) PSEUDO-ETIOLATION IN LIGHT (OsPEL) microProtein family members function as dominant-negative regulators of chloroplast development and are conserved among land plants. Knockout of all three OsPEL genes enhanced plant greening traits and was accompanied by leaf anatomical modifications associated with chloroplast-enriched bundle sheath cells in rice. These phenotypic changes correlated with increased CO2 assimilation efficiency and yield. OsPEL1 specifically interacts with key positive regulators of photosynthesis, the rice GOLDEN2-LIKE (OsGLK) transcription factors and the PHOTOSYSTEM I ASSEMBLY 2 (OsPSA2) chaperone. OsPEL1 inhibits these regulators by sequestering OsGLK1 and OsPSA2 in the cytoplasm, which prevents their proper localization to the nucleus and chloroplast, respectively. Supported by RNA-seq evidence of transcriptional homeostasis in greening-related genes, we reveal a multilayered regulatory mechanism and identify the OsPEL family as a promising target for crop improvement.","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"50 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2025-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Editing of rice PSEUDO-ETIOLATION IN LIGHT microProtein genes promotes chloroplast development\",\"authors\":\"Heebak Choi, Tae Gyu Yi, Yun-Shil Gho, Ki-Hong Jung, Sun-Hwa Ha\",\"doi\":\"10.1093/plcell/koaf235\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The rice (Oryza sativa) PSEUDO-ETIOLATION IN LIGHT (OsPEL) microProtein family members function as dominant-negative regulators of chloroplast development and are conserved among land plants. Knockout of all three OsPEL genes enhanced plant greening traits and was accompanied by leaf anatomical modifications associated with chloroplast-enriched bundle sheath cells in rice. These phenotypic changes correlated with increased CO2 assimilation efficiency and yield. OsPEL1 specifically interacts with key positive regulators of photosynthesis, the rice GOLDEN2-LIKE (OsGLK) transcription factors and the PHOTOSYSTEM I ASSEMBLY 2 (OsPSA2) chaperone. OsPEL1 inhibits these regulators by sequestering OsGLK1 and OsPSA2 in the cytoplasm, which prevents their proper localization to the nucleus and chloroplast, respectively. Supported by RNA-seq evidence of transcriptional homeostasis in greening-related genes, we reveal a multilayered regulatory mechanism and identify the OsPEL family as a promising target for crop improvement.\",\"PeriodicalId\":501012,\"journal\":{\"name\":\"The Plant Cell\",\"volume\":\"50 1\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2025-10-07\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"The Plant Cell\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1093/plcell/koaf235\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"The Plant Cell","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1093/plcell/koaf235","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
水稻（Oryza sativa） pseudoetiolation IN LIGHT （OsPEL）微蛋白家族成员是叶绿体发育的显性负调控因子，在陆地植物中是保守的。敲除所有三个OsPEL基因增强了水稻植株的绿化性状，并伴随着与叶绿体富集的束鞘细胞相关的叶片解剖改变。这些表型变化与CO2同化效率和产量的增加相关。OsPEL1特异性地与光合作用的关键正调控因子、水稻GOLDEN2-LIKE （OsGLK）转录因子和光系统I组装2 （OsPSA2）伴侣蛋白相互作用。OsPEL1通过在细胞质中隔离OsGLK1和OsPSA2来抑制这些调节因子，从而阻止它们分别正确定位到细胞核和叶绿体。在绿植相关基因转录稳态的RNA-seq证据的支持下，我们揭示了一个多层调控机制，并确定OsPEL家族是作物改良的一个有希望的目标。
Editing of rice PSEUDO-ETIOLATION IN LIGHT microProtein genes promotes chloroplast development
The rice (Oryza sativa) PSEUDO-ETIOLATION IN LIGHT (OsPEL) microProtein family members function as dominant-negative regulators of chloroplast development and are conserved among land plants. Knockout of all three OsPEL genes enhanced plant greening traits and was accompanied by leaf anatomical modifications associated with chloroplast-enriched bundle sheath cells in rice. These phenotypic changes correlated with increased CO2 assimilation efficiency and yield. OsPEL1 specifically interacts with key positive regulators of photosynthesis, the rice GOLDEN2-LIKE (OsGLK) transcription factors and the PHOTOSYSTEM I ASSEMBLY 2 (OsPSA2) chaperone. OsPEL1 inhibits these regulators by sequestering OsGLK1 and OsPSA2 in the cytoplasm, which prevents their proper localization to the nucleus and chloroplast, respectively. Supported by RNA-seq evidence of transcriptional homeostasis in greening-related genes, we reveal a multilayered regulatory mechanism and identify the OsPEL family as a promising target for crop improvement.
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