刚地弓形虫MIC13:潜在的候选疫苗基因——一种计算机方法

IF 1.8 Q3 PARASITOLOGY
Journal of Parasitology Research Pub Date : 2025-09-26 eCollection Date: 2025-01-01 DOI:10.1155/japr/3114690
Mahdi Khadem Mohammadi, Ali Dalir Ghaffari
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引用次数: 0

摘要

目的:弓形虫病是由刚地弓形虫(弓形虫)感染引起的,是一种广泛存在的寄生虫病,影响全球约三分之一的人口。常规药物并不能根除被感染宿主体内包囊内的寄生虫。MIC13被认为在促进寄生虫在整个宿主生物中的传播方面具有重要作用。本研究的目的是利用免疫信息学技术对MIC13蛋白进行抗原分析和结构预测,目的是确定可能用于制造弓形虫疫苗的潜在表位。材料与方法:目前的研究旨在描述MIC13蛋白的化学和物理特征、亚细胞定位、B细胞和t细胞的潜在表位、跨膜结构域、三级和二级结构等属性。结果:MIC13蛋白具有1个n -糖基化区、15个o -糖基化区、70个磷酸化位点和5个乙酰化位点,其结构中未检测到跨膜结构域。二级结构方面,MIC13蛋白由27.99%的α -螺旋、16.45%的延伸链和55.56%的随机线圈元件组成。此外,MIC13蛋白的各种潜在B细胞和t细胞表位被确定,表明其免疫原性。抗原性和致敏性的评估进一步证实MIC13具有免疫原性,但不具有致敏性,是一个有希望进一步研究的候选者。此外,IFN-γ和IL-4的诱导突出了相关MHC-II分子与MIC13相互作用的能力,表明其在免疫应答中的潜在作用。这些发现揭示了MIC13蛋白的多面性及其在免疫过程中的重要性。结论:这些发现表明MIC13可能是成功研制针对弓形虫的疫苗的关键组成部分。从这项研究中获得的结果为未来的调查奠定了坚实的基础,并为通过各种策略创造成功的疫苗来对抗急性和慢性弓形虫病提供了宝贵的见解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
MIC13 of Toxoplasma gondii: Potential Gene for Vaccine Candidate-An In Silico Approach.

Purpose: Toxoplasmosis, which is the result of infection by Toxoplasma gondii (T. gondii), is a widespread parasitic disease that affects approximately one-third of the global population. Routine medications are not able to eradicate the parasites enclosed in cysts that reside inside the hosts that are infected. MIC13 is believed to have a significant function in facilitating the dissemination of the parasite throughout the host organism. The objective of this research was to utilize immunoinformatics techniques for antigenic analysis and structural prediction of the MIC13 protein, with the goal of identifying potential epitopes that could be used to create a vaccine for T. gondii. Materials and Methods: The current research was aimed at describing the chemical and physical features, subcellular localization, potential epitopes for B- and T-cells, transmembrane domain, tertiary and secondary structures, and other attributes of the MIC13 protein. Results: The results indicated that the MIC13 protein possesses a single N-glycosylation, 15 O-glycosylation regions, 70 phosphorylation sites, and five acetylation sites, with no transmembrane domains being detected within its structure. In terms of secondary structure, the MIC13 protein is composed of 27.99% alpha-helix, 16.45% extended strand, and 55.56% random coil elements. Additionally, various potential B- and T-cell epitopes were pinpointed for the MIC13 protein, suggesting its immunogenic properties. The assessment of antigenicity and allergenicity further confirmed that MIC13 is immunogenic but nonallergenic, making it a promising candidate for further study. Furthermore, the induction of IFN-γ and IL-4 highlighted the ability of related MHC-II molecules to interact with MIC13, indicating its potential role in immune responses. These findings shed light on the multifaceted nature of the MIC13 protein and its significance in immunological processes. Conclusion: These findings suggest that MIC13 could serve as a key component in the creation of a successful vaccine targeting T. gondii. The results obtained from this research lay a solid foundation for future investigations and offer valuable insights for the creation of successful vaccines to combat both acute and chronic toxoplasmosis through diverse strategies.

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来源期刊
Journal of Parasitology Research
Journal of Parasitology Research Medicine-Infectious Diseases
CiteScore
3.50
自引率
9.10%
发文量
49
审稿时长
13 weeks
期刊介绍: Journal of Parasitology Research is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies in all areas of basic and applied parasitology. Articles covering host-parasite relationships and parasitic diseases will be considered, as well as studies on disease vectors. Articles highlighting social and economic issues around the impact of parasites are also encouraged. As an international, Open Access publication, Journal of Parasitology Research aims to foster learning and collaboration between countries and communities.
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