{"title":"阳离子/H+交换剂OsCHX11和OsCHX16促进钾在盐水和盐碱条件下的运输。","authors":"Mami Nampei, Daichi Toyama, Mitsuki Kondo, Nguyen Manh Linh, Akihiro Ueda","doi":"10.1093/bbb/zbaf142","DOIUrl":null,"url":null,"abstract":"<p><p>This study aimed to elucidate the functions of saline-alkaline inducible genes encoding OsCHX11 and OsCHX16, members of the cation/H+ exchanger (CHXs) family, under different component of saline-alkaline conditions. Rice biomass under carbonate-based (50 mM Na+ with carbonates) and high-pH (50 mM Na+ without carbonates) conditions was similar, whereas higher Na+/K+ ratio was observed under carbonate-based conditions. Under carbonate-based conditions, only OsCHX16 was significantly expressed, whereas both OsCHX11 and OsCHX16 were highly expressed under high pH conditions. The yeast complementation assay showed that OsCHX11 and OsCHX16 improved the yeast growth under saline, carbonate-based, and high-pH conditions by increasing K+ concentration. Taken together, these results suggest that OsCHX11 and OsCHX16 may contribute to the K+ uptake system under saline-alkaline conditions with or without carbonates at cell level.</p>","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":""},"PeriodicalIF":1.3000,"publicationDate":"2025-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Cation/H+ exchangers OsCHX11 and OsCHX16 facilitate potassium transport under saline and saline-alkaline conditions.\",\"authors\":\"Mami Nampei, Daichi Toyama, Mitsuki Kondo, Nguyen Manh Linh, Akihiro Ueda\",\"doi\":\"10.1093/bbb/zbaf142\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>This study aimed to elucidate the functions of saline-alkaline inducible genes encoding OsCHX11 and OsCHX16, members of the cation/H+ exchanger (CHXs) family, under different component of saline-alkaline conditions. Rice biomass under carbonate-based (50 mM Na+ with carbonates) and high-pH (50 mM Na+ without carbonates) conditions was similar, whereas higher Na+/K+ ratio was observed under carbonate-based conditions. Under carbonate-based conditions, only OsCHX16 was significantly expressed, whereas both OsCHX11 and OsCHX16 were highly expressed under high pH conditions. The yeast complementation assay showed that OsCHX11 and OsCHX16 improved the yeast growth under saline, carbonate-based, and high-pH conditions by increasing K+ concentration. Taken together, these results suggest that OsCHX11 and OsCHX16 may contribute to the K+ uptake system under saline-alkaline conditions with or without carbonates at cell level.</p>\",\"PeriodicalId\":9175,\"journal\":{\"name\":\"Bioscience, Biotechnology, and Biochemistry\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":1.3000,\"publicationDate\":\"2025-10-06\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Bioscience, Biotechnology, and Biochemistry\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://doi.org/10.1093/bbb/zbaf142\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bioscience, Biotechnology, and Biochemistry","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1093/bbb/zbaf142","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0
摘要
本研究旨在阐明阳离子/H+交换剂（CHXs）家族成员OsCHX11和OsCHX16的碱碱诱导基因在不同碱碱条件下的功能。碳酸盐基（50 mM Na+含碳酸盐）和高ph （50 mM Na+不含碳酸盐）条件下的水稻生物量相似，但碳酸盐基条件下Na+/K+比值较高。在碳酸盐基条件下，只有OsCHX16显著表达，而在高pH条件下，OsCHX11和OsCHX16均高表达。酵母互补实验表明，OsCHX11和OsCHX16通过提高K+浓度，在盐水、碳酸盐和高ph条件下促进酵母生长。综上所述，这些结果表明OsCHX11和OsCHX16可能在细胞水平上参与盐碱条件下有或没有碳酸盐的K+吸收系统。
Cation/H+ exchangers OsCHX11 and OsCHX16 facilitate potassium transport under saline and saline-alkaline conditions.
This study aimed to elucidate the functions of saline-alkaline inducible genes encoding OsCHX11 and OsCHX16, members of the cation/H+ exchanger (CHXs) family, under different component of saline-alkaline conditions. Rice biomass under carbonate-based (50 mM Na+ with carbonates) and high-pH (50 mM Na+ without carbonates) conditions was similar, whereas higher Na+/K+ ratio was observed under carbonate-based conditions. Under carbonate-based conditions, only OsCHX16 was significantly expressed, whereas both OsCHX11 and OsCHX16 were highly expressed under high pH conditions. The yeast complementation assay showed that OsCHX11 and OsCHX16 improved the yeast growth under saline, carbonate-based, and high-pH conditions by increasing K+ concentration. Taken together, these results suggest that OsCHX11 and OsCHX16 may contribute to the K+ uptake system under saline-alkaline conditions with or without carbonates at cell level.
期刊介绍：
Bioscience, Biotechnology, and Biochemistry publishes high-quality papers providing chemical and biological analyses of vital phenomena exhibited by animals, plants, and microorganisms, the chemical structures and functions of their products, and related matters. The Journal plays a major role in communicating to a global audience outstanding basic and applied research in all fields subsumed by the Japan Society for Bioscience, Biotechnology, and Agrochemistry (JSBBA).
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