抗霉素A,而不是抗霉素A3或粘噻唑,直接抑制光系统II的活性。

IF 1.3 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Ko Imaizumi, Kentaro Ifuku
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引用次数: 0

摘要

抗霉素A (antiycin A, AA)是一种广泛应用于光合作用和呼吸作用研究的抑制剂。在光合作用中,它通常用于抑制光系统I (CEF-PSI)周围的循环电子流途径,但也有报道影响光系统II (PSII),而不参与CEF-PSI。尽管人们对AA的特异性提出了担忧,但其对PSII活性的影响仍不清楚。AA3最近被认为是同一CEF-PSI途径的一种更特异性的抑制剂。在线粒体呼吸链中,AA抑制复合体III,如粘噻唑。本研究利用离体植物PSII和类囊体膜,研究了AA、AA3和粘噻唑对PSII活性和线性光合电子传递的直接影响。AA直接抑制PSII活性和线性电子传递,但AA3和粘噻唑均未抑制PSII活性。此外,AA的影响程度是批次依赖的。因此,我们建议用AA3来抑制CEF-PSI,用粘噻唑来抑制配合物III,而不是AA。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Antimycin A, but not antimycin A3 or myxothiazol, directly suppresses photosystem II activity.

Antimycin A (AA) is a widely used inhibitor to study photosynthesis and respiration. In photosynthesis, it is commonly used to inhibit a pathway of cyclic electron flow around photosystem I (CEF-PSI), but has also been reported to affect photosystem II (PSII), not involved in CEF-PSI. Although concerns have been raised about AA's specificity, its impact on PSII activity remains unclear. AA3 was recently proposed as a more specific inhibitor of the same CEF-PSI pathway. In the mitochondrial respiratory chain, AA inhibits complex III, like myxothiazol. Here, we investigated the direct effects of AA, AA3, and myxothiazol on PSII activity and linear photosynthetic electron transport using isolated plant PSII and thylakoid membranes. AA, but neither AA3 nor myxothiazol, directly suppressed PSII activity and linear electron transport. Furthermore, the extent of AA's effects was batch-dependent. Thus, we propose using AA3 to inhibit CEF-PSI and myxothiazol to inhibit complex III, instead of AA.

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来源期刊
Bioscience, Biotechnology, and Biochemistry
Bioscience, Biotechnology, and Biochemistry 生物-生化与分子生物学
CiteScore
3.50
自引率
0.00%
发文量
183
审稿时长
1 months
期刊介绍: Bioscience, Biotechnology, and Biochemistry publishes high-quality papers providing chemical and biological analyses of vital phenomena exhibited by animals, plants, and microorganisms, the chemical structures and functions of their products, and related matters. The Journal plays a major role in communicating to a global audience outstanding basic and applied research in all fields subsumed by the Japan Society for Bioscience, Biotechnology, and Agrochemistry (JSBBA).
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