{"title":"抗霉素A,而不是抗霉素A3或粘噻唑,直接抑制光系统II的活性。","authors":"Ko Imaizumi, Kentaro Ifuku","doi":"10.1093/bbb/zbaf141","DOIUrl":null,"url":null,"abstract":"<p><p>Antimycin A (AA) is a widely used inhibitor to study photosynthesis and respiration. In photosynthesis, it is commonly used to inhibit a pathway of cyclic electron flow around photosystem I (CEF-PSI), but has also been reported to affect photosystem II (PSII), not involved in CEF-PSI. Although concerns have been raised about AA's specificity, its impact on PSII activity remains unclear. AA3 was recently proposed as a more specific inhibitor of the same CEF-PSI pathway. In the mitochondrial respiratory chain, AA inhibits complex III, like myxothiazol. Here, we investigated the direct effects of AA, AA3, and myxothiazol on PSII activity and linear photosynthetic electron transport using isolated plant PSII and thylakoid membranes. AA, but neither AA3 nor myxothiazol, directly suppressed PSII activity and linear electron transport. Furthermore, the extent of AA's effects was batch-dependent. Thus, we propose using AA3 to inhibit CEF-PSI and myxothiazol to inhibit complex III, instead of AA.</p>","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":""},"PeriodicalIF":1.3000,"publicationDate":"2025-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Antimycin A, but not antimycin A3 or myxothiazol, directly suppresses photosystem II activity.\",\"authors\":\"Ko Imaizumi, Kentaro Ifuku\",\"doi\":\"10.1093/bbb/zbaf141\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Antimycin A (AA) is a widely used inhibitor to study photosynthesis and respiration. In photosynthesis, it is commonly used to inhibit a pathway of cyclic electron flow around photosystem I (CEF-PSI), but has also been reported to affect photosystem II (PSII), not involved in CEF-PSI. Although concerns have been raised about AA's specificity, its impact on PSII activity remains unclear. AA3 was recently proposed as a more specific inhibitor of the same CEF-PSI pathway. In the mitochondrial respiratory chain, AA inhibits complex III, like myxothiazol. Here, we investigated the direct effects of AA, AA3, and myxothiazol on PSII activity and linear photosynthetic electron transport using isolated plant PSII and thylakoid membranes. AA, but neither AA3 nor myxothiazol, directly suppressed PSII activity and linear electron transport. Furthermore, the extent of AA's effects was batch-dependent. Thus, we propose using AA3 to inhibit CEF-PSI and myxothiazol to inhibit complex III, instead of AA.</p>\",\"PeriodicalId\":9175,\"journal\":{\"name\":\"Bioscience, Biotechnology, and Biochemistry\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":1.3000,\"publicationDate\":\"2025-10-06\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Bioscience, Biotechnology, and Biochemistry\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://doi.org/10.1093/bbb/zbaf141\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bioscience, Biotechnology, and Biochemistry","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1093/bbb/zbaf141","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
Antimycin A, but not antimycin A3 or myxothiazol, directly suppresses photosystem II activity.
Antimycin A (AA) is a widely used inhibitor to study photosynthesis and respiration. In photosynthesis, it is commonly used to inhibit a pathway of cyclic electron flow around photosystem I (CEF-PSI), but has also been reported to affect photosystem II (PSII), not involved in CEF-PSI. Although concerns have been raised about AA's specificity, its impact on PSII activity remains unclear. AA3 was recently proposed as a more specific inhibitor of the same CEF-PSI pathway. In the mitochondrial respiratory chain, AA inhibits complex III, like myxothiazol. Here, we investigated the direct effects of AA, AA3, and myxothiazol on PSII activity and linear photosynthetic electron transport using isolated plant PSII and thylakoid membranes. AA, but neither AA3 nor myxothiazol, directly suppressed PSII activity and linear electron transport. Furthermore, the extent of AA's effects was batch-dependent. Thus, we propose using AA3 to inhibit CEF-PSI and myxothiazol to inhibit complex III, instead of AA.
期刊介绍:
Bioscience, Biotechnology, and Biochemistry publishes high-quality papers providing chemical and biological analyses of vital phenomena exhibited by animals, plants, and microorganisms, the chemical structures and functions of their products, and related matters. The Journal plays a major role in communicating to a global audience outstanding basic and applied research in all fields subsumed by the Japan Society for Bioscience, Biotechnology, and Agrochemistry (JSBBA).