{"title":"靶向沉默自驱动DNA行走器，用于金纳米颗粒固定抗体的小分子检测。","authors":"Han Pang, Qiang Zhao","doi":"10.1016/j.talanta.2025.128932","DOIUrl":null,"url":null,"abstract":"<p><p>A three-dimensional DNA walker offers an efficient strategy for sensitive detection of analytes with signal amplification. In this study, we report a target-silent, self-driven DNA walker for detecting small molecules (SMs). The DNA walker is composed of the Mg<sup>2+</sup>-dependent 8-17E DNAzyme walking strand conjugated with an SM (W-SM) and the three-dimensional walking track on gold nanoparticle (AuNP). The AuNP surface is functionalized with monoclonal antibody (mAb) and fluorescently labeled substrate of DNAzyme. Without target molecules, the W-SM is attached to the surface of AuNP via the antigen-antibody interaction. The DNAzyme catalytically cleaves the substrate, driving the W-SM autonomously moving along the walking track and generating high fluorescence. In the presence of SM target, the SM target competes with the W-SM in binding with the antibody on AuNP, and the DNA walker becomes inactive, causing fluorescence decline. This DNA walker enabled detection of digoxin and folic acid at concentrations as low as 0.2nM and 1 nM, respectively. It also performed well in diluted serum samples in responding to targets. This proposed strategy provides a new approach for constructing a DNA walker with a simple design for sensitive detection of small molecules in solution phase, showing promise in applications.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"298 Pt A","pages":"128932"},"PeriodicalIF":6.1000,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Target-silent self-driven DNA walker for small molecule detection with antibody immobilized on gold nanoparticles.\",\"authors\":\"Han Pang, Qiang Zhao\",\"doi\":\"10.1016/j.talanta.2025.128932\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>A three-dimensional DNA walker offers an efficient strategy for sensitive detection of analytes with signal amplification. In this study, we report a target-silent, self-driven DNA walker for detecting small molecules (SMs). The DNA walker is composed of the Mg<sup>2+</sup>-dependent 8-17E DNAzyme walking strand conjugated with an SM (W-SM) and the three-dimensional walking track on gold nanoparticle (AuNP). The AuNP surface is functionalized with monoclonal antibody (mAb) and fluorescently labeled substrate of DNAzyme. Without target molecules, the W-SM is attached to the surface of AuNP via the antigen-antibody interaction. The DNAzyme catalytically cleaves the substrate, driving the W-SM autonomously moving along the walking track and generating high fluorescence. In the presence of SM target, the SM target competes with the W-SM in binding with the antibody on AuNP, and the DNA walker becomes inactive, causing fluorescence decline. This DNA walker enabled detection of digoxin and folic acid at concentrations as low as 0.2nM and 1 nM, respectively. It also performed well in diluted serum samples in responding to targets. This proposed strategy provides a new approach for constructing a DNA walker with a simple design for sensitive detection of small molecules in solution phase, showing promise in applications.</p>\",\"PeriodicalId\":435,\"journal\":{\"name\":\"Talanta\",\"volume\":\"298 Pt A\",\"pages\":\"128932\"},\"PeriodicalIF\":6.1000,\"publicationDate\":\"2025-09-30\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Talanta\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://doi.org/10.1016/j.talanta.2025.128932\",\"RegionNum\":1,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, ANALYTICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Talanta","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1016/j.talanta.2025.128932","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
Target-silent self-driven DNA walker for small molecule detection with antibody immobilized on gold nanoparticles.
A three-dimensional DNA walker offers an efficient strategy for sensitive detection of analytes with signal amplification. In this study, we report a target-silent, self-driven DNA walker for detecting small molecules (SMs). The DNA walker is composed of the Mg2+-dependent 8-17E DNAzyme walking strand conjugated with an SM (W-SM) and the three-dimensional walking track on gold nanoparticle (AuNP). The AuNP surface is functionalized with monoclonal antibody (mAb) and fluorescently labeled substrate of DNAzyme. Without target molecules, the W-SM is attached to the surface of AuNP via the antigen-antibody interaction. The DNAzyme catalytically cleaves the substrate, driving the W-SM autonomously moving along the walking track and generating high fluorescence. In the presence of SM target, the SM target competes with the W-SM in binding with the antibody on AuNP, and the DNA walker becomes inactive, causing fluorescence decline. This DNA walker enabled detection of digoxin and folic acid at concentrations as low as 0.2nM and 1 nM, respectively. It also performed well in diluted serum samples in responding to targets. This proposed strategy provides a new approach for constructing a DNA walker with a simple design for sensitive detection of small molecules in solution phase, showing promise in applications.
期刊介绍：
Talanta provides a forum for the publication of original research papers, short communications, and critical reviews in all branches of pure and applied analytical chemistry. Papers are evaluated based on established guidelines, including the fundamental nature of the study, scientific novelty, substantial improvement or advantage over existing technology or methods, and demonstrated analytical applicability. Original research papers on fundamental studies, and on novel sensor and instrumentation developments, are encouraged. Novel or improved applications in areas such as clinical and biological chemistry, environmental analysis, geochemistry, materials science and engineering, and analytical platforms for omics development are welcome.
Analytical performance of methods should be determined, including interference and matrix effects, and methods should be validated by comparison with a standard method, or analysis of a certified reference material. Simple spiking recoveries may not be sufficient. The developed method should especially comprise information on selectivity, sensitivity, detection limits, accuracy, and reliability. However, applying official validation or robustness studies to a routine method or technique does not necessarily constitute novelty. Proper statistical treatment of the data should be provided. Relevant literature should be cited, including related publications by the authors, and authors should discuss how their proposed methodology compares with previously reported methods.
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