Bruna Estefânia Diniz Frias, Roberta Oliveira Prado, Mariana Gontijo Ramos, Nathália Werneck Cézar de Oliveira, Fernanda Fortes de Araújo, Liliane Martins Dos Santos, Camila Gontijo Ramos, Camila Bechara Kallás, Maria Sílvia Laborne Alves de Sousa, Ismael Artur Costa-Rocha, Joaquim Pedro Brito-de-Sousa, Rachel Basques Caligiorne, Marcelo Antônio Pascoal-Xavier, Vanessa Peruhype-Magalhães, Daniel Gontijo Ramos, Andréa Teixeira-Carvalho, Olindo Assis Martins-Filho
{"title":"在接受自体角质细胞/黑素细胞移植的稳定型白癜风患者中，免疫特征驱动不同的再色素沉着模式。","authors":"Bruna Estefânia Diniz Frias, Roberta Oliveira Prado, Mariana Gontijo Ramos, Nathália Werneck Cézar de Oliveira, Fernanda Fortes de Araújo, Liliane Martins Dos Santos, Camila Gontijo Ramos, Camila Bechara Kallás, Maria Sílvia Laborne Alves de Sousa, Ismael Artur Costa-Rocha, Joaquim Pedro Brito-de-Sousa, Rachel Basques Caligiorne, Marcelo Antônio Pascoal-Xavier, Vanessa Peruhype-Magalhães, Daniel Gontijo Ramos, Andréa Teixeira-Carvalho, Olindo Assis Martins-Filho","doi":"10.1016/j.imlet.2025.107098","DOIUrl":null,"url":null,"abstract":"<p><p>This study aimed to characterize biomarkers of therapeutic response in patients with stable vitiligo undergoing the autologous non-cultured keratinocyte/melanocyte transplantation. The approaches performed were systemic analysis evaluated during pre-transplantation/(D0), seven/(D7), and 28/(D28-45) days after the procedure and compartmentalized analysis in epidermal cell suspension (explant). Increased number of pro-inflammatory monocytes and NK-cells was found during the transplantation follow-up in vitiligo patients compared to the control. There were higher numbers of CD8+ T-cells and CD4+HLA-DR+ T-cells circulating at D28-45 compared to D0, and increased number of CD8+HLA-DR+ T-cells at D28-45 compared to D7. Decreased levels of the most pro-inflammatory chemokines/cytokines during post-transplantation kinetics timeline were observed. Integrative analysis demonstrated that patients with unsatisfactory repigmentation presented higher numbers of connections between the blood/skin components at D0. The data suggest differentiated profiles in the dynamics of the hematological/immunological biomarkers, according to the kinetics timeline and the clinical outcome of repigmentation in vitiligo patients.</p>","PeriodicalId":13413,"journal":{"name":"Immunology letters","volume":" ","pages":"107098"},"PeriodicalIF":2.8000,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Immunological features driving distinct repigmentation patterns in patients with stable vitiligo submitted to the autologous keratinocyte/melanocyte transplantation.\",\"authors\":\"Bruna Estefânia Diniz Frias, Roberta Oliveira Prado, Mariana Gontijo Ramos, Nathália Werneck Cézar de Oliveira, Fernanda Fortes de Araújo, Liliane Martins Dos Santos, Camila Gontijo Ramos, Camila Bechara Kallás, Maria Sílvia Laborne Alves de Sousa, Ismael Artur Costa-Rocha, Joaquim Pedro Brito-de-Sousa, Rachel Basques Caligiorne, Marcelo Antônio Pascoal-Xavier, Vanessa Peruhype-Magalhães, Daniel Gontijo Ramos, Andréa Teixeira-Carvalho, Olindo Assis Martins-Filho\",\"doi\":\"10.1016/j.imlet.2025.107098\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>This study aimed to characterize biomarkers of therapeutic response in patients with stable vitiligo undergoing the autologous non-cultured keratinocyte/melanocyte transplantation. The approaches performed were systemic analysis evaluated during pre-transplantation/(D0), seven/(D7), and 28/(D28-45) days after the procedure and compartmentalized analysis in epidermal cell suspension (explant). Increased number of pro-inflammatory monocytes and NK-cells was found during the transplantation follow-up in vitiligo patients compared to the control. There were higher numbers of CD8+ T-cells and CD4+HLA-DR+ T-cells circulating at D28-45 compared to D0, and increased number of CD8+HLA-DR+ T-cells at D28-45 compared to D7. Decreased levels of the most pro-inflammatory chemokines/cytokines during post-transplantation kinetics timeline were observed. Integrative analysis demonstrated that patients with unsatisfactory repigmentation presented higher numbers of connections between the blood/skin components at D0. 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Immunological features driving distinct repigmentation patterns in patients with stable vitiligo submitted to the autologous keratinocyte/melanocyte transplantation.
This study aimed to characterize biomarkers of therapeutic response in patients with stable vitiligo undergoing the autologous non-cultured keratinocyte/melanocyte transplantation. The approaches performed were systemic analysis evaluated during pre-transplantation/(D0), seven/(D7), and 28/(D28-45) days after the procedure and compartmentalized analysis in epidermal cell suspension (explant). Increased number of pro-inflammatory monocytes and NK-cells was found during the transplantation follow-up in vitiligo patients compared to the control. There were higher numbers of CD8+ T-cells and CD4+HLA-DR+ T-cells circulating at D28-45 compared to D0, and increased number of CD8+HLA-DR+ T-cells at D28-45 compared to D7. Decreased levels of the most pro-inflammatory chemokines/cytokines during post-transplantation kinetics timeline were observed. Integrative analysis demonstrated that patients with unsatisfactory repigmentation presented higher numbers of connections between the blood/skin components at D0. The data suggest differentiated profiles in the dynamics of the hematological/immunological biomarkers, according to the kinetics timeline and the clinical outcome of repigmentation in vitiligo patients.
期刊介绍：
Immunology Letters provides a vehicle for the speedy publication of experimental papers, (mini)Reviews and Letters to the Editor addressing all aspects of molecular and cellular immunology. The essential criteria for publication will be clarity, experimental soundness and novelty. Results contradictory to current accepted thinking or ideas divergent from actual dogmas will be considered for publication provided that they are based on solid experimental findings.
Preference will be given to papers of immediate importance to other investigators, either by their experimental data, new ideas or new methodology. Scientific correspondence to the Editor-in-Chief related to the published papers may also be accepted provided that they are short and scientifically relevant to the papers mentioned, in order to provide a continuing forum for discussion.
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