氯四环素对嗜热厌氧污泥发酵过程中挥发性脂肪酸生成的影响机制及影响的新认识

IF 3 4区环境科学与生态学 Q2 ENVIRONMENTAL SCIENCES

Water, Air, & Soil Pollution Pub Date : 2025-10-06 DOI:10.1007/s11270-025-08675-8

Jiabao Liao, Jianwei Zhao

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引用次数: 0

摘要

抗生素污染阻碍了污泥资源的利用，然而，在嗜热厌氧系统中，氯四环素（CTC）的作用及其对挥发性脂肪酸（VFA）产生的影响尚不清楚。本研究通过研究CTC对嗜热厌氧污泥发酵的剂量依赖性影响及其潜在机制来填补这一空白。结果表明，低浓度（50 ~ 100 mg/kg） CTC具有提高VFA产率的双重作用，其中最优浓度（100 mg/kg）可使VFA峰值提高26.3%（达到245.6 mg/g VSS），醋酸酯占75%以上。这是由ctc激活的细胞外水解酶驱动的，促进溶解有机物释放（可溶性化学需氧量峰值5915 mg/L）和醋酸盐主导途径。相反，高浓度（≥150 mg/kg）可使VFA降低25.3%，并通过抑制水解诱导丙酸积累（18.1%）。在机制上，CTC通过调节EPS调节系统稳定性，低剂量增加可溶性/松散结合的EPS，减少紧密结合的EPS，增强水解酶的可及性。本研究创新性地阐明了CTC在嗜热发酵过程中的剂量依赖性调控，为基于浓度阈值优化抗生素污染污泥资源化利用提供了理论框架。图形抽象

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New Insights into the Influence Mechanisms and Impacts of Chlortetracycline on Volatile Fatty Acid Generation during Thermophilic Anaerobic Sludge Fermentation

Antibiotic contamination hinders sludge resource utilization, yet the role of chlortetracycline (CTC) in thermophilic anaerobic systems and its impact on volatile fatty acid (VFA) production remain unclear. This study fills this gap by investigating CTC’s dose-dependent effects on thermophilic anaerobic sludge fermentation and underlying mechanisms. Results revealed a dual role of CTC: low concentrations (50–100 mg/kg) enhanced VFA yields, with the optimal 100 mg/kg increasing peak VFA by 26.3% (to 245.6 mg/g VSS) and acetate accounting for over 75%. This was driven by CTC-activated extracellular hydrolases, promoting dissolved organic matter release (soluble chemical oxygen demand peak 5915 mg/L) and acetate-dominant pathways. Conversely, high concentrations (≥ 150 mg/kg) reduced VFA by 25.3% and induced propionate accumulation (18.1%) via suppressed hydrolysis. Mechanistically, CTC regulated system stability by modulating EPS, low doses increased soluble/loosely bound EPS and reduced tightly bound EPS, enhancing hydrolase accessibility. This study innovatively clarifies CTC’s dose-dependent regulation in thermophilic fermentation, providing a theoretical framework for optimizing antibiotic-contaminated sludge resource utilization based on concentration thresholds.

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来源期刊

Water, Air, & Soil Pollution 环境科学-环境科学

CiteScore

4.50

自引率

6.90%

发文量

448

审稿时长

2.6 months

期刊介绍： Water, Air, & Soil Pollution is an international, interdisciplinary journal on all aspects of pollution and solutions to pollution in the biosphere. This includes chemical, physical and biological processes affecting flora, fauna, water, air and soil in relation to environmental pollution. Because of its scope, the subject areas are diverse and include all aspects of pollution sources, transport, deposition, accumulation, acid precipitation, atmospheric pollution, metals, aquatic pollution including marine pollution and ground water, waste water, pesticides, soil pollution, sewage, sediment pollution, forestry pollution, effects of pollutants on humans, vegetation, fish, aquatic species, micro-organisms, and animals, environmental and molecular toxicology applied to pollution research, biosensors, global and climate change, ecological implications of pollution and pollution models. Water, Air, & Soil Pollution also publishes manuscripts on novel methods used in the study of environmental pollutants, environmental toxicology, environmental biology, novel environmental engineering related to pollution, biodiversity as influenced by pollution, novel environmental biotechnology as applied to pollution (e.g. bioremediation), environmental modelling and biorestoration of polluted environments. Articles should not be submitted that are of local interest only and do not advance international knowledge in environmental pollution and solutions to pollution. Articles that simply replicate known knowledge or techniques while researching a local pollution problem will normally be rejected without review. Submitted articles must have up-to-date references, employ the correct experimental replication and statistical analysis, where needed and contain a significant contribution to new knowledge. The publishing and editorial team sincerely appreciate your cooperation. Water, Air, & Soil Pollution publishes research papers; review articles; mini-reviews; and book reviews.