{"title":"化学发光免疫法与液相色谱-串联质谱法测定不同病理生理条件下高血压患者肾素-血管紧张素-醛固酮水平的比较。","authors":"Ting-Ting Xu, Yi-Gui Tang, En-Jun Xu, Xuan-Xuan Wang, Yu-Ping Yang, Zeng Guo","doi":"10.1038/s41371-025-01071-z","DOIUrl":null,"url":null,"abstract":"<p><p>This study aimed to compare measurements of the renin-angiotensin-aldosterone system (RAAS) components by liquid chromatography-tandem mass spectrometry (LC-MS/MS) and chemiluminescent immunoassay (CLIA) in hypertensive patients with different gender, position and renal function. EDTA plasma from 100 hypertensive patients who completed postural test were included. Aldosterone (PAC), renin (PRC, using Autobio CLIA microparticles kit; DRC, using LIAISON Direct Renin CLIA kit), angiotensin I and II (AngI and AngII), 18-hydroxycorticosterone (18-OHB) and 18-hydroxycortisol (18-OHF) were measured. Median PAC<sub>CLIA</sub> was 46.0% higher (P < 0.01) than PAC<sub>LC-MS/MS</sub>. Median PAC<sub>CLIA</sub>, 18-OHB<sub>LC-MS/MS</sub> and 18-OHF<sub>LC-MS/MS</sub> were higher (P < 0.01) in patients with renal dysfunction compared with patients with normal renal function, whereas median PAC<sub>LC-MS/MS</sub> did not show a significant difference between the two groups. Both median PRC<sub>CLIA</sub> and DRC<sub>CLIA</sub> were higher (P < 0.01) in male patients compared with female patients. PAC<sub>LC-MS/MS</sub>, PAC<sub>CLIA</sub>, PRC<sub>CLIA</sub> and DRC<sub>CLIA</sub> all showed good consistency in response to an assumption of upright posture. Close (P < 0.01) correlations were observed between PAC<sub>CLIA</sub> and PAC<sub>LC-MS/MS</sub>, between PRC<sub>CLIA</sub> and DRC<sub>CLIA</sub>, between AngII<sub>LC-MS/MS</sub> and PRC<sub>CLIA</sub>, and between AngII<sub>LC-MS/MS</sub> and DRC<sub>CLIA</sub>. LC-MS/MS is a reliable assay for measurement of the RAAS components in hypertensive patients under different pathophysiological conditions.</p>","PeriodicalId":16070,"journal":{"name":"Journal of Human Hypertension","volume":" ","pages":""},"PeriodicalIF":3.4000,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Comparisons of renin-angiotensin-aldosterone levels measured by chemical luminescence immunoassay and liquid chromatography-tandem mass spectrometry in hypertensive patients under different pathophysiological conditions.\",\"authors\":\"Ting-Ting Xu, Yi-Gui Tang, En-Jun Xu, Xuan-Xuan Wang, Yu-Ping Yang, Zeng Guo\",\"doi\":\"10.1038/s41371-025-01071-z\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>This study aimed to compare measurements of the renin-angiotensin-aldosterone system (RAAS) components by liquid chromatography-tandem mass spectrometry (LC-MS/MS) and chemiluminescent immunoassay (CLIA) in hypertensive patients with different gender, position and renal function. EDTA plasma from 100 hypertensive patients who completed postural test were included. Aldosterone (PAC), renin (PRC, using Autobio CLIA microparticles kit; DRC, using LIAISON Direct Renin CLIA kit), angiotensin I and II (AngI and AngII), 18-hydroxycorticosterone (18-OHB) and 18-hydroxycortisol (18-OHF) were measured. Median PAC<sub>CLIA</sub> was 46.0% higher (P < 0.01) than PAC<sub>LC-MS/MS</sub>. Median PAC<sub>CLIA</sub>, 18-OHB<sub>LC-MS/MS</sub> and 18-OHF<sub>LC-MS/MS</sub> were higher (P < 0.01) in patients with renal dysfunction compared with patients with normal renal function, whereas median PAC<sub>LC-MS/MS</sub> did not show a significant difference between the two groups. Both median PRC<sub>CLIA</sub> and DRC<sub>CLIA</sub> were higher (P < 0.01) in male patients compared with female patients. PAC<sub>LC-MS/MS</sub>, PAC<sub>CLIA</sub>, PRC<sub>CLIA</sub> and DRC<sub>CLIA</sub> all showed good consistency in response to an assumption of upright posture. Close (P < 0.01) correlations were observed between PAC<sub>CLIA</sub> and PAC<sub>LC-MS/MS</sub>, between PRC<sub>CLIA</sub> and DRC<sub>CLIA</sub>, between AngII<sub>LC-MS/MS</sub> and PRC<sub>CLIA</sub>, and between AngII<sub>LC-MS/MS</sub> and DRC<sub>CLIA</sub>. LC-MS/MS is a reliable assay for measurement of the RAAS components in hypertensive patients under different pathophysiological conditions.</p>\",\"PeriodicalId\":16070,\"journal\":{\"name\":\"Journal of Human Hypertension\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":3.4000,\"publicationDate\":\"2025-10-03\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Human Hypertension\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1038/s41371-025-01071-z\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"PERIPHERAL VASCULAR DISEASE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Human Hypertension","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1038/s41371-025-01071-z","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"PERIPHERAL VASCULAR DISEASE","Score":null,"Total":0}
Comparisons of renin-angiotensin-aldosterone levels measured by chemical luminescence immunoassay and liquid chromatography-tandem mass spectrometry in hypertensive patients under different pathophysiological conditions.
This study aimed to compare measurements of the renin-angiotensin-aldosterone system (RAAS) components by liquid chromatography-tandem mass spectrometry (LC-MS/MS) and chemiluminescent immunoassay (CLIA) in hypertensive patients with different gender, position and renal function. EDTA plasma from 100 hypertensive patients who completed postural test were included. Aldosterone (PAC), renin (PRC, using Autobio CLIA microparticles kit; DRC, using LIAISON Direct Renin CLIA kit), angiotensin I and II (AngI and AngII), 18-hydroxycorticosterone (18-OHB) and 18-hydroxycortisol (18-OHF) were measured. Median PACCLIA was 46.0% higher (P < 0.01) than PACLC-MS/MS. Median PACCLIA, 18-OHBLC-MS/MS and 18-OHFLC-MS/MS were higher (P < 0.01) in patients with renal dysfunction compared with patients with normal renal function, whereas median PACLC-MS/MS did not show a significant difference between the two groups. Both median PRCCLIA and DRCCLIA were higher (P < 0.01) in male patients compared with female patients. PACLC-MS/MS, PACCLIA, PRCCLIA and DRCCLIA all showed good consistency in response to an assumption of upright posture. Close (P < 0.01) correlations were observed between PACCLIA and PACLC-MS/MS, between PRCCLIA and DRCCLIA, between AngIILC-MS/MS and PRCCLIA, and between AngIILC-MS/MS and DRCCLIA. LC-MS/MS is a reliable assay for measurement of the RAAS components in hypertensive patients under different pathophysiological conditions.
期刊介绍：
Journal of Human Hypertension is published monthly and is of interest to health care professionals who deal with hypertension (specialists, internists, primary care physicians) and public health workers. We believe that our patients benefit from robust scientific data that are based on well conducted clinical trials. We also believe that basic sciences are the foundations on which we build our knowledge of clinical conditions and their management. Towards this end, although we are primarily a clinical based journal, we also welcome suitable basic sciences studies that promote our understanding of human hypertension.
The journal aims to perform the dual role of increasing knowledge in the field of high blood pressure as well as improving the standard of care of patients. The editors will consider for publication all suitable papers dealing directly or indirectly with clinical aspects of hypertension, including but not limited to epidemiology, pathophysiology, therapeutics and basic sciences involving human subjects or tissues. We also consider papers from all specialties such as ophthalmology, cardiology, nephrology, obstetrics and stroke medicine that deal with the various aspects of hypertension and its complications.
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