{"title":"从海洋软体动物中分离出的韩国Aureibaculum sp. 11 .和conchae sp. 11 .。","authors":"Soo-Bin Kim, Kyung Hyun Kim, Jin-Sook Park","doi":"10.1099/ijsem.0.006933","DOIUrl":null,"url":null,"abstract":"<p><p>Facultative anaerobic Gram-negative bacteria, designated strains 2210JD6-5<sup>T</sup> and 2308TA14-22<sup>T</sup>, were isolated from a seashell and a marine conch, respectively, in the Republic of Korea, and subjected to detailed taxonomic analysis. The cells exhibited catalase and oxidase activity, were non-motile and were rod-shaped. Both novel strains were mesophilic, grew optimally at neutral pH and were slightly halophilic. Phylogenetic analyses based on 16S rRNA gene and whole-genome sequences demonstrated that strains 2210JD6-5<sup>T</sup> and 2308TA14-22<sup>T</sup> were affiliated with the genus <i>Aureibaculum</i>. The digital DNA-DNA hybridization and average nucleotide identity values between strains 2210JD6-5<sup>T</sup> and 2308TA14-22<sup>T</sup> and type strains of the most closely related species were below the cut-off thresholds. Both strains possessed DNA G+C contents of 33.5 mol%. The predominant cellular fatty acids and the sole respiratory quinone in these two strains were iso-C<sub>15 : 0</sub>, iso-C<sub>15 : 1</sub> G and iso-C<sub>17 : 0</sub> 3-OH and MK-6, respectively. Strains 2210JD6-5<sup>T</sup> and 2308TA14-22<sup>T</sup> shared polar lipids, such as phosphoethanolamine, aminophospholipid, unidentified aminolipid and unidentified lipid. Based on phylogenetic, genomic relatedness and chemotaxonomic characteristics, strains 2210JD6-5<sup>T</sup> and 2308TA14-22<sup>T</sup> represent a novel species within the genus <i>Aureibaculum</i>, for which the names <i>Aureibaculum koreense</i> sp. nov. (2210JD6-5<sup>T</sup>=KCTC 102167<sup>T</sup>=JCM 37322<sup>T</sup>) and <i>Aureibaculum conchae</i> sp. nov. (2308TA14-22<sup>T</sup>=KCTC 102250<sup>T</sup>=JCM 37321<sup>T</sup>) are proposed.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 10","pages":""},"PeriodicalIF":2.0000,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"<i>Aureibaculum koreense</i> sp. nov. and <i>Aureibaculum conchae</i> sp. nov., isolated from the marine molluscs.\",\"authors\":\"Soo-Bin Kim, Kyung Hyun Kim, Jin-Sook Park\",\"doi\":\"10.1099/ijsem.0.006933\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Facultative anaerobic Gram-negative bacteria, designated strains 2210JD6-5<sup>T</sup> and 2308TA14-22<sup>T</sup>, were isolated from a seashell and a marine conch, respectively, in the Republic of Korea, and subjected to detailed taxonomic analysis. The cells exhibited catalase and oxidase activity, were non-motile and were rod-shaped. Both novel strains were mesophilic, grew optimally at neutral pH and were slightly halophilic. Phylogenetic analyses based on 16S rRNA gene and whole-genome sequences demonstrated that strains 2210JD6-5<sup>T</sup> and 2308TA14-22<sup>T</sup> were affiliated with the genus <i>Aureibaculum</i>. The digital DNA-DNA hybridization and average nucleotide identity values between strains 2210JD6-5<sup>T</sup> and 2308TA14-22<sup>T</sup> and type strains of the most closely related species were below the cut-off thresholds. Both strains possessed DNA G+C contents of 33.5 mol%. The predominant cellular fatty acids and the sole respiratory quinone in these two strains were iso-C<sub>15 : 0</sub>, iso-C<sub>15 : 1</sub> G and iso-C<sub>17 : 0</sub> 3-OH and MK-6, respectively. Strains 2210JD6-5<sup>T</sup> and 2308TA14-22<sup>T</sup> shared polar lipids, such as phosphoethanolamine, aminophospholipid, unidentified aminolipid and unidentified lipid. Based on phylogenetic, genomic relatedness and chemotaxonomic characteristics, strains 2210JD6-5<sup>T</sup> and 2308TA14-22<sup>T</sup> represent a novel species within the genus <i>Aureibaculum</i>, for which the names <i>Aureibaculum koreense</i> sp. nov. (2210JD6-5<sup>T</sup>=KCTC 102167<sup>T</sup>=JCM 37322<sup>T</sup>) and <i>Aureibaculum conchae</i> sp. nov. (2308TA14-22<sup>T</sup>=KCTC 102250<sup>T</sup>=JCM 37321<sup>T</sup>) are proposed.</p>\",\"PeriodicalId\":14390,\"journal\":{\"name\":\"International journal of systematic and evolutionary microbiology\",\"volume\":\"75 10\",\"pages\":\"\"},\"PeriodicalIF\":2.0000,\"publicationDate\":\"2025-10-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International journal of systematic and evolutionary microbiology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1099/ijsem.0.006933\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International journal of systematic and evolutionary microbiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1099/ijsem.0.006933","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
Aureibaculum koreense sp. nov. and Aureibaculum conchae sp. nov., isolated from the marine molluscs.
Facultative anaerobic Gram-negative bacteria, designated strains 2210JD6-5T and 2308TA14-22T, were isolated from a seashell and a marine conch, respectively, in the Republic of Korea, and subjected to detailed taxonomic analysis. The cells exhibited catalase and oxidase activity, were non-motile and were rod-shaped. Both novel strains were mesophilic, grew optimally at neutral pH and were slightly halophilic. Phylogenetic analyses based on 16S rRNA gene and whole-genome sequences demonstrated that strains 2210JD6-5T and 2308TA14-22T were affiliated with the genus Aureibaculum. The digital DNA-DNA hybridization and average nucleotide identity values between strains 2210JD6-5T and 2308TA14-22T and type strains of the most closely related species were below the cut-off thresholds. Both strains possessed DNA G+C contents of 33.5 mol%. The predominant cellular fatty acids and the sole respiratory quinone in these two strains were iso-C15 : 0, iso-C15 : 1 G and iso-C17 : 0 3-OH and MK-6, respectively. Strains 2210JD6-5T and 2308TA14-22T shared polar lipids, such as phosphoethanolamine, aminophospholipid, unidentified aminolipid and unidentified lipid. Based on phylogenetic, genomic relatedness and chemotaxonomic characteristics, strains 2210JD6-5T and 2308TA14-22T represent a novel species within the genus Aureibaculum, for which the names Aureibaculum koreense sp. nov. (2210JD6-5T=KCTC 102167T=JCM 37322T) and Aureibaculum conchae sp. nov. (2308TA14-22T=KCTC 102250T=JCM 37321T) are proposed.
期刊介绍:
Published by the Microbiology Society and owned by the International Committee on Systematics of Prokaryotes (ICSP), a committee of the Bacteriology and Applied Microbiology Division of the International Union of Microbiological Societies, International Journal of Systematic and Evolutionary Microbiology is the leading forum for the publication of novel microbial taxa and the ICSP’s official journal of record for prokaryotic names.
The journal welcomes high-quality research on all aspects of microbial evolution, phylogenetics and systematics, encouraging submissions on all prokaryotes, yeasts, microfungi, protozoa and microalgae across the full breadth of systematics including:
Identification, characterisation and culture preservation
Microbial evolution and biodiversity
Molecular environmental work with strong taxonomic or evolutionary content
Nomenclature
Taxonomy and phylogenetics.