个性化海藻酸盐包封:自体血液添加剂在甲状旁腺细胞移植中的作用。

IF 9.6 2区 医学 Q1 ENGINEERING, BIOMEDICAL
Özge Karabıyık Acar, Gülnihal Bozdağ, Ahmed Alperen Tuncer, Ezgi Hacıhasanoğlu, Lal Era Aydoğan, Gülçin Delal Nozhatzadeh, Fikrettin Şahin, Erhan Aysan, Gamze Torun Köse
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引用次数: 0

摘要

甲状旁腺功能减退的唯一治疗干预是甲状旁腺移植,但移植排斥反应是一个问题。本研究试图通过在移植中使用患者的血液、血清或血浆来缓解这一问题。为了实现这一目标,从Sprague-Dawley大鼠身上提取的血液添加剂被掺入海藻酸盐中,人类甲状旁腺细胞被包裹起来。对制备的微珠进行力学性能监测,然后将其异种移植到大鼠体内,以评估细胞功能和免疫反应。生物降解数据表明,含有血液和血浆的微珠的结构完整性优于血清,而含有血浆的微珠的耐久性仅与仅含有海藻酸盐的微珠相当。包括血浆在内的微珠释放出最高水平的甲状旁腺激素(PTH),无论是在体外还是在体内。这种行为可能归因于血浆在调节免疫反应的同时对细胞功能的有益影响。血液掺入引起免疫反应升高,同时对被包裹的细胞提供最小的支持。CCL2 (MCP-1)趋化因子水平在血液和仅海藻酸盐微球组中均显著升高,与CD68表达相关。这些发现表明,自体血浆添加可以调节免疫系统,从而降低细胞移植排斥反应的风险。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Personalized Alginate Encapsulation: The Role of Autologous Blood Additives in Parathyroid Cell Transplantation.

The only therapeutic intervention for hypoparathyroidism is parathyroid transplantation, but graft rejection is a concern. This study sought to mitigate this problem by utilizing the patient's blood, serum, or plasma in the transplant. To accomplish this objective, blood additives derived from Sprague-Dawley rats are incorporated within alginate, and human parathyroid cells are encapsulated. The prepared microbeads are monitored for mechanical properties, followed by xenotransplantation into rats for the evaluation of cell function and immunological response. Biodegradation data showed that the structural integrity of the microbeads containing blood and plasma is superior to serum, while the durability of plasma-including microbeads is only comparable to that of the alginate-only group. Plasma-including microbeads released the highest levels of parathyroid hormone (PTH), both in vitro and in vivo. This behavior could be attributed to the beneficial impact of plasma on cellular function while regulating immune response. Blood incorporation provoked an elevated immune response while concurrently offering minimal support to the encapsulated cells. A notable elevation in CCL2 (MCP-1) chemokine levels is observed in both blood and alginate-only microbead groups, correlating with CD68 expression. These findings demonstrated that autologous plasma addition may regulate the immune system, thereby diminishing the risk of rejection in cell transplantations.

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来源期刊
Advanced Healthcare Materials
Advanced Healthcare Materials 工程技术-生物材料
CiteScore
14.40
自引率
3.00%
发文量
600
审稿时长
1.8 months
期刊介绍: Advanced Healthcare Materials, a distinguished member of the esteemed Advanced portfolio, has been dedicated to disseminating cutting-edge research on materials, devices, and technologies for enhancing human well-being for over ten years. As a comprehensive journal, it encompasses a wide range of disciplines such as biomaterials, biointerfaces, nanomedicine and nanotechnology, tissue engineering, and regenerative medicine.
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