Özge Karabıyık Acar, Gülnihal Bozdağ, Ahmed Alperen Tuncer, Ezgi Hacıhasanoğlu, Lal Era Aydoğan, Gülçin Delal Nozhatzadeh, Fikrettin Şahin, Erhan Aysan, Gamze Torun Köse
{"title":"个性化海藻酸盐包封:自体血液添加剂在甲状旁腺细胞移植中的作用。","authors":"Özge Karabıyık Acar, Gülnihal Bozdağ, Ahmed Alperen Tuncer, Ezgi Hacıhasanoğlu, Lal Era Aydoğan, Gülçin Delal Nozhatzadeh, Fikrettin Şahin, Erhan Aysan, Gamze Torun Köse","doi":"10.1002/adhm.202501483","DOIUrl":null,"url":null,"abstract":"<p><p>The only therapeutic intervention for hypoparathyroidism is parathyroid transplantation, but graft rejection is a concern. This study sought to mitigate this problem by utilizing the patient's blood, serum, or plasma in the transplant. To accomplish this objective, blood additives derived from Sprague-Dawley rats are incorporated within alginate, and human parathyroid cells are encapsulated. The prepared microbeads are monitored for mechanical properties, followed by xenotransplantation into rats for the evaluation of cell function and immunological response. Biodegradation data showed that the structural integrity of the microbeads containing blood and plasma is superior to serum, while the durability of plasma-including microbeads is only comparable to that of the alginate-only group. Plasma-including microbeads released the highest levels of parathyroid hormone (PTH), both in vitro and in vivo. This behavior could be attributed to the beneficial impact of plasma on cellular function while regulating immune response. Blood incorporation provoked an elevated immune response while concurrently offering minimal support to the encapsulated cells. A notable elevation in CCL2 (MCP-1) chemokine levels is observed in both blood and alginate-only microbead groups, correlating with CD68 expression. These findings demonstrated that autologous plasma addition may regulate the immune system, thereby diminishing the risk of rejection in cell transplantations.</p>","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e01483"},"PeriodicalIF":9.6000,"publicationDate":"2025-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Personalized Alginate Encapsulation: The Role of Autologous Blood Additives in Parathyroid Cell Transplantation.\",\"authors\":\"Özge Karabıyık Acar, Gülnihal Bozdağ, Ahmed Alperen Tuncer, Ezgi Hacıhasanoğlu, Lal Era Aydoğan, Gülçin Delal Nozhatzadeh, Fikrettin Şahin, Erhan Aysan, Gamze Torun Köse\",\"doi\":\"10.1002/adhm.202501483\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The only therapeutic intervention for hypoparathyroidism is parathyroid transplantation, but graft rejection is a concern. This study sought to mitigate this problem by utilizing the patient's blood, serum, or plasma in the transplant. To accomplish this objective, blood additives derived from Sprague-Dawley rats are incorporated within alginate, and human parathyroid cells are encapsulated. The prepared microbeads are monitored for mechanical properties, followed by xenotransplantation into rats for the evaluation of cell function and immunological response. Biodegradation data showed that the structural integrity of the microbeads containing blood and plasma is superior to serum, while the durability of plasma-including microbeads is only comparable to that of the alginate-only group. Plasma-including microbeads released the highest levels of parathyroid hormone (PTH), both in vitro and in vivo. This behavior could be attributed to the beneficial impact of plasma on cellular function while regulating immune response. Blood incorporation provoked an elevated immune response while concurrently offering minimal support to the encapsulated cells. A notable elevation in CCL2 (MCP-1) chemokine levels is observed in both blood and alginate-only microbead groups, correlating with CD68 expression. These findings demonstrated that autologous plasma addition may regulate the immune system, thereby diminishing the risk of rejection in cell transplantations.</p>\",\"PeriodicalId\":113,\"journal\":{\"name\":\"Advanced Healthcare Materials\",\"volume\":\" \",\"pages\":\"e01483\"},\"PeriodicalIF\":9.6000,\"publicationDate\":\"2025-10-04\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Advanced Healthcare Materials\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://doi.org/10.1002/adhm.202501483\",\"RegionNum\":2,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"ENGINEERING, BIOMEDICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Advanced Healthcare Materials","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1002/adhm.202501483","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENGINEERING, BIOMEDICAL","Score":null,"Total":0}
Personalized Alginate Encapsulation: The Role of Autologous Blood Additives in Parathyroid Cell Transplantation.
The only therapeutic intervention for hypoparathyroidism is parathyroid transplantation, but graft rejection is a concern. This study sought to mitigate this problem by utilizing the patient's blood, serum, or plasma in the transplant. To accomplish this objective, blood additives derived from Sprague-Dawley rats are incorporated within alginate, and human parathyroid cells are encapsulated. The prepared microbeads are monitored for mechanical properties, followed by xenotransplantation into rats for the evaluation of cell function and immunological response. Biodegradation data showed that the structural integrity of the microbeads containing blood and plasma is superior to serum, while the durability of plasma-including microbeads is only comparable to that of the alginate-only group. Plasma-including microbeads released the highest levels of parathyroid hormone (PTH), both in vitro and in vivo. This behavior could be attributed to the beneficial impact of plasma on cellular function while regulating immune response. Blood incorporation provoked an elevated immune response while concurrently offering minimal support to the encapsulated cells. A notable elevation in CCL2 (MCP-1) chemokine levels is observed in both blood and alginate-only microbead groups, correlating with CD68 expression. These findings demonstrated that autologous plasma addition may regulate the immune system, thereby diminishing the risk of rejection in cell transplantations.
期刊介绍:
Advanced Healthcare Materials, a distinguished member of the esteemed Advanced portfolio, has been dedicated to disseminating cutting-edge research on materials, devices, and technologies for enhancing human well-being for over ten years. As a comprehensive journal, it encompasses a wide range of disciplines such as biomaterials, biointerfaces, nanomedicine and nanotechnology, tissue engineering, and regenerative medicine.