利用新一代3D癌症模型阐明肿瘤-微生物组串扰。

IF 9.6 2区 医学 Q1 ENGINEERING, BIOMEDICAL
Marina Green Buzhor, Giuseppe Longobardi, Or Kandli, Anne Krinsky, Opal Avramoff, Anshika Katyal, Koren Salomon, Adan Miari, Dana Venkert, Tania T Barnatan, América García Alvarado, Shahar Greenberg, Ronit Satchi-Fainaro
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引用次数: 0

摘要

肿瘤微环境(TME)是一个复杂而动态的生态系统,因其与微生物群的相互作用而日益得到认可。在结直肠癌、乳腺癌、肺癌、肝癌和脑癌中,细菌群落及其代谢物被证明影响肿瘤进展、免疫反应和治疗结果。为了在生理学相关背景下研究这些相互作用,先进的3D体外模型已经出现,包括球体、类器官、微流控器官芯片平台和生物3D打印构建体。这些系统提供了空间组织、机械线索和共培养能力,促进了宿主-微生物组-肿瘤串扰的研究。将活细菌、它们的代谢物和免疫成分结合到这些平台中,对微生物群如何塑造癌症行为、炎症和耐药性产生了新的见解。本文概述了用于研究肿瘤-微生物组相互作用的3D模型开发的最新进展,重点介绍了器官特异性应用、细胞外基质模拟水凝胶和生物制造策略。它还解决了关键挑战,包括维持微生物组活力,建模时间动态和整合免疫复杂性。克服这些限制需要跨学科的方法,融合生物工程,微生物学和肿瘤学。不断发展的3D平台为微生物组信息的癌症建模提供了强大的工具,并为推进治疗筛选和精确肿瘤学提供了重要的希望。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Harnessing Next-Generation 3D Cancer Models to Elucidate Tumor-Microbiome Crosstalk.

The tumor microenvironment (TME) is a complex and dynamic ecosystem increasingly recognized for its interplay with the microbiome. In colorectal, breast, lung, liver, and brain cancers, bacterial communities and their metabolites are shown to influence tumor progression, immune responses, and therapeutic outcomes. To study these interactions in physiologically relevant contexts, advanced 3D in vitro models have emerged, including spheroids, organoids, microfluidic organ-on-a-chip platforms, and 3D-bioprinted constructs. These systems provide spatial organization, mechanical cues, and co-culture capabilities that facilitate investigation of host-microbiome-tumor cross-talk. Incorporation of live bacteria, their metabolites, and immune components into these platforms has yielded new insights into how the microbiome shapes cancer behavior, inflammation, and drug resistance. This review outlines recent advances in 3D model development for studying tumor-microbiome interactions, highlighting organ-specific applications, extracellular matrix-mimicking hydrogels, and biofabrication strategies. It also addresses key challenges, including maintaining microbiome viability, modeling temporal dynamics, and integrating immune complexity. Overcoming these limitations requires interdisciplinary approaches that merge bioengineering, microbiology, and oncology. Evolving 3D platforms offer powerful tools for microbiome-informed cancer modeling and hold significant promise for advancing therapeutic screening and precision oncology.

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来源期刊
Advanced Healthcare Materials
Advanced Healthcare Materials 工程技术-生物材料
CiteScore
14.40
自引率
3.00%
发文量
600
审稿时长
1.8 months
期刊介绍: Advanced Healthcare Materials, a distinguished member of the esteemed Advanced portfolio, has been dedicated to disseminating cutting-edge research on materials, devices, and technologies for enhancing human well-being for over ten years. As a comprehensive journal, it encompasses a wide range of disciplines such as biomaterials, biointerfaces, nanomedicine and nanotechnology, tissue engineering, and regenerative medicine.
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