山药红肉中生物活性成分的综合分析。由UHPLC-Q-Orbitrap-HRMS

IF 1.7 4区 医学 Q4 BIOCHEMICAL RESEARCH METHODS
Lanping Yang, Wendi Wang, Zhenzhen Liu, Yangyang Zhai, Zhenhui Wang, Ying Li, Zhenzhen Zhang, Baohua Hou, Baobao Zhang, Jingchun Zhou, Dehong Guo, Miaoqing Zhou, Wei Guo
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引用次数: 0

摘要

薯蓣。简历。铁根(DTT)是日常食品和药品。然而,由于黄芪的营养植物化学成分尚不清楚,在去除黄芪红肉方面存在混淆。本研究旨在揭示RF的植物化学成分和抗氧化能力。采用超高效液相色谱-四极杆轨道rap高分辨率质谱法(UHPLC-Q-Orbitrap HRMS)测定其综合植物化学成分,并对提取物的抗氧化活性(DPPH、ABTS和FRAP)进行评价。共检测到249种植物化学物质,包括150种初级代谢物,68种次级代谢物和31种其他化合物。值得注意的是,与WF(白肉)相比,RF中鉴定出166种差异代谢物(163种上调)。RF显著提高了初级代谢物(如脂类、有机酸和氨基酸)和次级代谢物(如黄酮类和酚酸)的含量。RF的抗氧化活性(DPPH、ABTS和FRAP)高于WF。相关分析发现,大部分差异代谢物与抗氧化能力呈显著正相关。这是第一次系统、全面地对黄芩植物化学成分进行定性分析。这项研究扩大了我们对RF生物活性成分的认识,并突出了DTT潜在的营养和药用价值。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Comprehensive Analysis of Bioactive Phytoconstituents in Red Flesh of Dioscorea opposita Thunb. by UHPLC-Q-Orbitrap-HRMS

Comprehensive Analysis of Bioactive Phytoconstituents in Red Flesh of Dioscorea opposita Thunb. by UHPLC-Q-Orbitrap-HRMS

Dioscorea opposita Thunb. cv. Tiegun (DTT) is used daily as food and medicine. However, there is confusion in removing the red flesh of DTT (RF), as the nutrient phytochemicals of RF are unclear. This study aimed to reveal the phytochemicals and antioxidant capabilities of RF. An ultra-high-performance liquid chromatography-quadrupole Orbitrap high-resolution mass spectrometry (UHPLC-Q-Orbitrap HRMS) approach was employed to determine the comprehensive phytochemicals of RF, and the antioxidant activities (DPPH, ABTS, and FRAP) of the extracts were evaluated. A total of 249 phytochemicals were detected, including 150 primary metabolites, 68 secondary metabolites, and 31 other compounds. Notably, 166 differential metabolites (163 upregulated) were identified in RF relative to WF (white flesh). RF significantly increased the contents of primary metabolites (such as lipids, organic acids, and amino acids) and secondary metabolites (such as flavonoids and phenolic acids). RF showed higher antioxidant activity (DPPH, ABTS, and FRAP) than WF. The correlation analysis identified that most of the differential metabolites were significantly positively correlated with the antioxidant capacity. This is the first systematic and comprehensive qualitative analysis of the phytochemicals of RF. This study expands our knowledge of the bioactive constituents of RF and highlights the potential nutritional and medicinal values of DTT.

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来源期刊
Biomedical Chromatography
Biomedical Chromatography 生物-分析化学
CiteScore
3.60
自引率
5.60%
发文量
268
审稿时长
2.3 months
期刊介绍: Biomedical Chromatography is devoted to the publication of original papers on the applications of chromatography and allied techniques in the biological and medical sciences. Research papers and review articles cover the methods and techniques relevant to the separation, identification and determination of substances in biochemistry, biotechnology, molecular biology, cell biology, clinical chemistry, pharmacology and related disciplines. These include the analysis of body fluids, cells and tissues, purification of biologically important compounds, pharmaco-kinetics and sequencing methods using HPLC, GC, HPLC-MS, TLC, paper chromatography, affinity chromatography, gel filtration, electrophoresis and related techniques.
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