磁性纳米粒子超宽带铁磁共振检测GDF15蛋白

IF 1.1 4区物理与天体物理 Q4 ENGINEERING, ELECTRICAL & ELECTRONIC

IEEE Magnetics Letters Pub Date : 2025-07-24 DOI:10.1109/LMAG.2025.3592474

Shin Yabukami;Ryoya Masui;Toru Murayama;Junichi Honda;Loi Tonthat;Kazuhiko Okita;Akihiro Kuwahata;Takaaki Abe

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引用次数: 0

摘要

本研究开发了一种生长分化因子-15 （GDF15）蛋白检测方法，该方法使用频率高达67 GHz的超宽带微带线型探针。我们使用一抗和二抗制备了结合GDF15和磁性纳米颗粒（MNPs）的样品。高密度的次级MNPs与抗原反应多次，利用生物素-亲和素反应在覆盖玻璃上增强磁信号。抗原与MNPs之间的反应通过荧光粒子和振动样品磁强计证实。直流磁场的应用增强了锐利铁磁共振（FMR），提高了GDF15检测的信噪比。随着GDF15浓度（0、0.01、0.1和1µg/ml）的增加，1.5和2 T间FMR强度的差异明显增大。获得了可重复的蛋白（GDF15）检测校准曲线。该方法有望在生物医学应用中实现灵敏、定量的蛋白质检测。

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Detection of GDF15 Protein Using Ultrabroadband Ferromagnetic Resonance of Magnetic Nanoparticles

This study developed a protein detection method of the growth differentiation factor-15 (GDF15) using a ultrabroadband microstrip line type probe up to 67 GHz. We prepared samples that bound the GDF15 and magnetic nanoparticles (MNPs) using primary and secondary antibodies. High-density secondary MNPs reacted with antigen several times to enhance the magnetic signal using a biotin–avidin reaction on a cover glass. The reaction between the antigen and the MNPs was confirmed by fluorescent particles and a vibrating sample magnetometer. The application of the dc field enhanced sharp ferromagnetic resonance (FMR), improving the signal-to-noise ratio for GDF15 detection. The difference in FMR intensity between 1.5 and 2 T increased clearly with increasing GDF15 concentration (0, 0.01, 0.1, and 1 µg/ml). A reproducible calibration curve was obtained for protein (GDF15) detection. Our method shows promise for sensitive and quantitative protein detection in biomedical applications.

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来源期刊

IEEE Magnetics Letters PHYSICS, APPLIED-

CiteScore

2.40

自引率

0.00%

发文量

期刊介绍： IEEE Magnetics Letters is a peer-reviewed, archival journal covering the physics and engineering of magnetism, magnetic materials, applied magnetics, design and application of magnetic devices, bio-magnetics, magneto-electronics, and spin electronics. IEEE Magnetics Letters publishes short, scholarly articles of substantial current interest. IEEE Magnetics Letters is a hybrid Open Access (OA) journal. For a fee, authors have the option making their articles freely available to all, including non-subscribers. OA articles are identified as Open Access.