绿色合成的氧化铜微海绵凝胶,装载红毛藤和杜鹃花,用于抗菌伤口愈合应用

Pavithra Bharathy , Elizabeth Rani Edwin , Jelis priya Nagarajan , Naveenraaj jeyaprakash , Punniyakoti Veeraveedu Thanikachalam
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引用次数: 0

摘要

研究背景:以红木和杜鹃花为原料合成氧化铜微海绵。花提取物已显示出作为新型抗菌剂的潜力。本研究旨在评价这些绿色合成的CuOMs的抗菌效果及其在伤口护理中的应用。方法以硝酸铜为原料,以植物提取物为还原剂和稳定剂,采用准乳状溶剂扩散法制备scum。采用UV、FTIR、XRD、SEM-EDX、Zeta浆料机和LC-MS对合成的微海绵进行了表征。采用琼脂孔扩散法、时间杀伤法和生化分析(包括蛋白质和细胞质渗漏)对伤口相关病原体(如铜绿假单胞菌、大肠杆菌、金黄色葡萄球菌和粪肠球菌)的抗菌活性进行了评估。结果CuOMs凝胶具有较强的抑菌活性,抑菌带范围为14 ~ 22 mm。LC-MS研究表明槲皮素和芦丁在这两种植物提取物中都对伤口愈合起重要作用。时间杀伤试验表明,在2 h内具有快速杀菌效果,特别是对铜绿假单胞菌。蛋白质和细胞质渗漏试验表明细菌膜明显破坏,证实了微海绵的作用机制。在所有检测的病原菌中,铜绿假单胞菌的易感性最高。结论生物合成的CuOMs具有较强的抗菌性能,能有效地破坏细菌细胞的完整性。将这些化合物结合到局部凝胶基质中,为伤口愈合和感染控制提供了一种有前途的策略。氧化铜和生物活性植物化合物的协同作用支持了它们作为抗菌伤口治疗安全有效替代品的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Green-synthesized copper oxide microsponge gel loaded with Ixora coccinea and Rhododendron arboreum for antibacterial wound healing applications

Background

Copper oxide microsponges (CuOMs) synthesized using Ixora coccinea L. and Rhododendron arboreum Sm. flower extracts have shown potential as novel antimicrobial agents. This study aims to evaluate the antibacterial efficacy of these green-synthesized CuOMs and their application in wound care.

Methods

CuOMs were fabricated via a quasi-emulsion solvent diffusion method using copper nitrate, with plant extracts serving as reducing and stabilizing agents. The synthesized microsponges were characterized using UV, FTIR, XRD, SEM-EDX, Zeta sizer and LC-MS. The antibacterial activity was assessed using agar well diffusion, time-kill assays and biochemical analyses including protein and cytoplasmic leakage against wound-associated pathogens such as Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus and Enterococcus faecalis.

Results

The CuOMs incorporated gel exhibited substantial antibacterial activity, with inhibition zones ranging from 14 to 22 mm. LC-MS studies shows presence of quercetin and rutin in both plant extract plays a major role in wound healing. Time-kill assays demonstrated rapid bactericidal effects within 2 h, particularly against P. aeruginosa. Protein and cytoplasmic leakage assays indicate significant disruption of bacterial membranes, confirming the microsponge mechanism of action. Among all the tested pathogens, P. aeruginosa showed the greatest susceptibility.

Conclusion

The biosynthesized CuOMs exhibited strong antibacterial properties and effectively disrupted bacterial cell integrity. The incorporation of these compounds into a topical gel matrix offers a promising strategy for wound healing and infection control. The synergistic effect of copper oxide and bioactive plant compounds supports their potential as safe and effective alternatives for antimicrobial wound therapy.
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