通过利用氢键诱导的发射来监测膀胱癌生物标志物透明质酸酶-1的uranalysis定制荧光策略

IF 10.5 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics Pub Date : 2025-10-01 DOI:10.1016/j.bios.2025.118060

Weiju Chen , Laicui Song , Jinqiu Luo , Jiaxuan Tang , Tianbing Ren , Sheng Yang , Jing Zheng , Ronghua Yang

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引用次数: 0

摘要

通过发光策略对尿液生物标志物进行无创监测在早期癌症的精确治疗中起着至关重要的作用。然而，开发高特异性和高灵敏度的分子分析工具仍然具有挑战性。利用尿液的富水特性，通过调节氢键诱导发射，提出了一种适合尿液分析的荧光策略。我们合理构建了一种基于自组装透明质酸的纳米传感器，该传感器可被膀胱癌潜在预后生物标志物透明质酸酶-1特异性解离，将捕获的具有抗溶剂致变色作用的查尔酮荧光团释放到亲水环境中，通过形成氢键组装诱导发光。设计的纳米传感器成功地用于根据不同的Hyal-1水平区分健康个体和膀胱癌患者。此外，这种方法将被推广到开发一个工具箱，用于更多疾病相关的感兴趣的生物标志物的尿液测试。

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Uranalysis-tailored fluorogenic strategy for monitoring bladder cancer biomarker hyaluronidase-1 by harnessing hydrogen-bonding-induced emission

Non-invasive monitoring of urinary biomarkers by luminescent strategy plays a critical role in enabling precise theranostics for early-stage cancer. However, the development of highly specific and sensitive molecular tools for uranalysis remains challenging. Leveraging aqueous-rich characteristic of urine, a uranalysis-tailored fluorogenic strategy was proposed by regulating hydrogen-bonding induced emission herein. We rationally constructed a self-assembly hyaluronic acid-based nanosensor that can be specifically dissociated by hyaluronidase-1, a potentially prognostic biomarker of bladder cancer, to release the entrapped chalcone fluorophores with anti-solvatochromic effect into hydrophilic environments, inducing bright emission through the formation of a hydrogen-bond assembly. The designed nanosensor was successfully used to distinguish healthy individuals and bladder cancer patients based on their differential Hyal-1 levels. Moreover, this approach would be generalized to develop a toolbox for urinary tests of more diseases-associated biomarkers of interest.

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来源期刊

Biosensors and Bioelectronics 工程技术-电化学

CiteScore

20.80

自引率

7.10%

发文量

1006

审稿时长

29 days

期刊介绍： Biosensors & Bioelectronics, along with its open access companion journal Biosensors & Bioelectronics: X, is the leading international publication in the field of biosensors and bioelectronics. It covers research, design, development, and application of biosensors, which are analytical devices incorporating biological materials with physicochemical transducers. These devices, including sensors, DNA chips, electronic noses, and lab-on-a-chip, produce digital signals proportional to specific analytes. Examples include immunosensors and enzyme-based biosensors, applied in various fields such as medicine, environmental monitoring, and food industry. The journal also focuses on molecular and supramolecular structures for enhancing device performance.