通过快速构建和筛选系统生成针对SARS-CoV-2和猴痘病毒的特异性人抗体

IF 2.3 4区 医学 Q3 IMMUNOLOGY
Guanglei Gu , Jinhai Wang , Xiaoyun Hu , Xiuling Gu , Shirui Li , Hanle Li , Min Fang
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引用次数: 0

摘要

单克隆抗体在现代医学中发挥着越来越重要的作用。人单克隆抗体由于其安全性已成为单克隆抗体治疗发展的一个重点。我们开发了一个综合平台,通过重组抗原生产、荧光抗原四聚体、记忆b细胞分选、单细胞基因扩增和单链抗体表达,快速构建和筛选人抗体,快速筛选抗体特异性。利用健康供者外周血单个核细胞,成功制备了针对SARS-CoV-2 N和S1蛋白的人单克隆抗体。此外,我们利用该平台制备了猴痘病毒(MPXV) M1R蛋白的保护性人抗体。这些结果表明,我们的系统能够快速有效地发现特异性单克隆抗体,为对抗新出现的传染病和推进癌症免疫治疗提供广泛的适用性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Specific human antibodies against SARS-CoV-2 and monkeypox virus generated via a rapid construction and screening system
Monoclonal antibodies are playing an increasingly important role in modern medicine. Human monoclonal antibodies have become a key focus in the development of monoclonal antibody therapeutics due to their safety. We developed an integrated platform that rapidly constructs and screens human antibodies by combining recombinant antigen production, fluorescent antigen tetramers, memory B-cell sorting, single-cell gene amplification, and single-chain antibody expression for rapid screening of antibody specificity. Using peripheral blood mononuclear cells from healthy donors, we successfully generated human monoclonal antibodies targeting the N and S1 proteins of SARS-CoV-2. Furthermore, we applied the platform to produce protective human antibodies against the M1R protein of the monkeypox virus (MPXV). These results demonstrate that our system enables the rapid and efficient discovery of specific monoclonal antibodies, offering broad applicability for combating emerging infectious diseases and advancing cancer immunotherapies.
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来源期刊
Immunobiology
Immunobiology 医学-免疫学
CiteScore
5.00
自引率
3.60%
发文量
108
审稿时长
55 days
期刊介绍: Immunobiology is a peer-reviewed journal that publishes highly innovative research approaches for a wide range of immunological subjects, including • Innate Immunity, • Adaptive Immunity, • Complement Biology, • Macrophage and Dendritic Cell Biology, • Parasite Immunology, • Tumour Immunology, • Clinical Immunology, • Immunogenetics, • Immunotherapy and • Immunopathology of infectious, allergic and autoimmune disease.
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