诱导型magg凝集素在苹果对火疫病免疫中的作用:CRISPR/Cas9验证及其内遗传方法的潜力

IF 8.5 1区 农林科学 Q1 Agricultural and Biological Sciences
Antoine Bodelot, Nicolas Dousset, Elisa Ravon, Christelle Heintz, Marie-Noelle Brisset, Alexandre Degrave, Emilie Vergne
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引用次数: 0

摘要

由淀粉Erwinia amylovora细菌引起的火疫病对苹果(Malus domestica)生产构成重大威胁。目前,仅确定了有限数量的有效参与淀粉样芽孢杆菌抗性的基因。为了寻找新的耐药候选物,我们重点研究了一个编码苋菜素样凝集素的多基因家族,该凝集素在酸性苯并- s -甲基(ASM)化学激发后高度上调。这些凝集素被认为通过促进细菌聚集有助于下游防御,这导致它们被命名为家蝇凝集素(MdAGG)。当mmdagg基因中引入功能缺失版本时,植物在ASM处理下对火疫病产生完全有效防御反应的能力受到损害,证实了mmdagg在抗火疫病中的作用。接下来,我们将苹果内源的pPPO16启动子与MdAGG10的编码序列偶联,获得了能诱导MdAGG10表达的苹果株系。mmdagg10在这些品系中的早期表达显著提高了对火疫病的抗性,额外的ASM处理进一步增强了这种抗性。综上所述,我们认为mmdaggs作为防御基因,其及时表达可以提供对淀粉样芽孢杆菌的有效抗性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Inducible MdAGG lectins in apple immunity toward Fire Blight: CRISPR/Cas9 validation and their potential for intragenesis approaches
Fire blight, caused by the bacterium Erwinia amylovora, represents a significant threat to apple (Malus domestica) production. Currently, only a limited number of genes effectively involved in resistance to E. amylovora have been identified. Seeking new resistance candidates, we focused on a multigene family encoding amaranthin-like lectins, which are highly upregulated following chemical elicitation by acibenzolar-S-methyl (ASM). These lectins are believed to contribute to downstream defense by promoting bacterial aggregation, which led to their designation as Malus domestica agglutinins (MdAGG). When loss-of-function editions were introduced into MdAGG genes, the plant's ability to mount a fully effective defense response against fire blight upon ASM treatment was compromised, confirming the role of MdAGGs in fire blight resistance. Next, we coupled the pPPO16 promoter, endogenous to apple and known to be rapidly induced during E. amylovora infection, with the coding sequence of MdAGG10 to create apple lines with fire blight-inducible MdAGG10 expression. Early MdAGG10 expression in these lines significantly improved resistance to fire blight, and an additional ASM treatment further enhanced this resistance. In summary, we conclude that MdAGGs act as defense genes whose timely expression can provide effective resistance against E. amylovora.
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来源期刊
Horticulture Research
Horticulture Research Biochemistry, Genetics and Molecular Biology-Biochemistry
CiteScore
11.20
自引率
6.90%
发文量
367
审稿时长
20 weeks
期刊介绍: Horticulture Research, an open access journal affiliated with Nanjing Agricultural University, has achieved the prestigious ranking of number one in the Horticulture category of the Journal Citation Reports ™ from Clarivate, 2022. As a leading publication in the field, the journal is dedicated to disseminating original research articles, comprehensive reviews, insightful perspectives, thought-provoking comments, and valuable correspondence articles and letters to the editor. Its scope encompasses all vital aspects of horticultural plants and disciplines, such as biotechnology, breeding, cellular and molecular biology, evolution, genetics, inter-species interactions, physiology, and the origination and domestication of crops.
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