α-二羰基化合物对鸡成肌细胞活力的影响。

IF 2.2 3区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology Pub Date : 2025-09-30 DOI:10.1016/j.cbpa.2025.111937

Meiko Okino , Ryosuke Makino , Tetsuya Tachibana

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引用次数: 0

摘要

α-二羰基化合物（α-DCs），如乙二醛（GO）和甲基乙二醛（MGO），是葡萄糖通过各种化学反应产生的高活性化合物。伴有慢性高血糖的糖尿病患者血液中GO和MGO的浓度高于健康人。氧化石墨烯和氧化石墨烯对多种哺乳动物细胞具有细胞毒性，并与糖尿病并发症有关。鉴于鸡是高血糖动物，它们的血液中会含有高水平的氧化石墨烯和氧化石墨烯。然而，氧化石墨烯和氧化石墨烯在鸡细胞中的细胞毒性作用尚未被阐明。本研究的目的是阐明氧化石墨烯和MGO对鸡成肌细胞活力的影响。两种α- dc降低了细胞内烟酰胺腺嘌呤二核苷酸（还原形式）的水平，而烟酰胺腺嘌呤二核苷酸是细胞活力、细胞增殖和能量代谢的指标。然而，两种α- dc均未诱导凋亡细胞死亡、活性氧产生或脂质过氧化。此外，氧化石墨烯和MGO均未诱导线粒体功能障碍。本研究表明，氧化石墨烯和MGO对鸡的肌肉生成有不利影响。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Effects of α-dicarbonyl compounds on cell viability in chicken myoblasts

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Effects of α-dicarbonyl compounds on cell viability in chicken myoblasts

Alpha-dicarbonyl compounds (α-DCs), such as glyoxal (GO) and methylglyoxal (MGO), are highly reactive compounds produced from glucose through various chemical reactions. Diabetic patients with chronic hyperglycemia exhibit higher blood concentrations of GO and MGO than healthy individuals. GO and MGO are cytotoxic to various mammalian cells and are associated with diabetic complications. Given that chickens are hyperglycemic animals, they would possess high levels of GO and MGO in their blood. However, the cytotoxic effects of GO and MGO have not been elucidated in chicken cells. The purpose of the present study was to clarify the effects of GO and MGO on the viability of chicken myoblasts. The two α-DCs decreased the level of intracellular nicotinamide adenine dinucleotide (reduced form), which is an indicator of cell viability, cell proliferation, and energy metabolism. However, neither of the two α-DCs induced apoptotic cell death, production of reactive oxygen species, or lipid peroxidation. In addition, GO and MGO did not induce mitochondrial dysfunction. The present study demonstrated that GO and MGO would have adverse effects on myogenesis in chickens.

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来源期刊

Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology 生物-动物学

CiteScore

5.00

自引率

4.30%

发文量

155

审稿时长

3 months

期刊介绍： Part A: Molecular & Integrative Physiology of Comparative Biochemistry and Physiology. This journal covers molecular, cellular, integrative, and ecological physiology. Topics include bioenergetics, circulation, development, excretion, ion regulation, endocrinology, neurobiology, nutrition, respiration, and thermal biology. Study on regulatory mechanisms at any level of organization such as signal transduction and cellular interaction and control of behavior are also published.