肾脏研究的创新技术：三维成像和量化。

IF 3.5 3区医学 Q2 UROLOGY & NEPHROLOGY

Seminars in nephrology Pub Date : 2025-10-01 DOI:10.1016/j.semnephrol.2025.151671

Sarah R McLarnon, Pierre-Emmanuel Y N'Guetta, Lori L O'Brien

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引用次数: 0

摘要

图像分析在我们了解肾脏形态、功能和疾病方面起着至关重要的作用。这种分析历来仅限于在二维上可视化肾脏内的定义区域。然而，近年来，显微镜技术的重大进步促进了大型组织标本的三维成像和分析，在某些情况下，整个器官或生物体。这些显微技术结合组织清除策略的使用，已经产生了肾脏复杂结构和过程的详细、多维视图。这篇综述讨论了先进的光学显微镜的应用和光学清除方案已经成功地修改用于肾脏。此外，这篇综述将强调三维图像的量化如何应用于肾脏，从而有助于新的时空见解。Semin Nephrol 36:x-xx©20XX Elsevier Inc.。版权所有。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Innovative Technologies for Kidney Research: Three-Dimensional Imaging and Quantification.

Image analysis has played a critical role in our understanding of kidney morphology, function, and disease. This analysis has been historically limited to visualizing defined regions within the kidney in two dimensions. However, in recent years, significant advancements in microscopy have facilitated three-dimensional imaging and analysis of large tissue specimens and, in some cases, whole organs or organism. The use of these microscopy techniques combined with tissue-clearing strategies has resulted in detailed, multidimensional views of complex structures and processes within the kidney. This review discusses advanced light microscopy applications and optical clearing protocols that have been successfully modified for use in the kidney. Furthermore, this review will highlight how quantification of three-dimensional images has been applied in the kidney and thus contributed to novel spatiotemporal insights. Semin Nephrol 36:x-xx © 20XX Elsevier Inc. All rights reserved.

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来源期刊

Seminars in nephrology 医学-泌尿学与肾脏学

CiteScore

5.60

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： Seminars in Nephrology is a timely source for the publication of new concepts and research findings relevant to the clinical practice of nephrology. Each issue is an organized compendium of practical information that serves as a lasting reference for nephrologists, internists and physicians in training.