{"title":"细菌对偶氮染料的降解：优化、动力学、降解途径、植物毒性和纺织废水处理","authors":"Mohanasundram Sudha, Arul Ponniah Saranya, Ganeshamoorthy Bakiyaraj, Mariappan Mariappan, Natesan Sivakumar","doi":"10.1007/s11270-025-08529-3","DOIUrl":null,"url":null,"abstract":"<div><p>Azo dyes are organic, artificial colorants with a wide range of applications as textile coloring agents. The disposal of textile effluent negatively impacts soil and water ecosystems; hence, enhancing the microbial degradation of azo dyes is essential for the textile industry's disposal treatment. The prime focus of this research is studying the bacterial degradation of azo textile dyes, Direct Green 28 (DG-28) and Direct Blue 71 (DB-71). Taxonomic studies of the isolated strain revealed that it was <i>Escherichia</i> sp. PP001 (GenBank: KP770133.1), and it has the potential to degrade azo dyes. The culture conditions were optimized under various physiological parameters such as dye concentrations, pH, temperature, and agitation. The maximum decolorization of 99% was achieved for both dyes at pH 7, 37 °C, with agitation of 60 rpm (revolutions per minute). Biosorption kinetics fit pseudo-zero-order and diffusion models, indicating surface adsorption and intraparticle diffusion. GC–MS (Gas Chromatography Mass Spectroscopy) analysis elucidated metabolite degradation pathways. Physicochemical properties of the treated and untreated textile effluent were examined and compared. The results indicate a reduction of 97.63% in biological oxygen demand (BOD), 96.23% in chemical oxygen demand (COD), 64.56% in total dissolved solids (TDS), 76.80% in total solids (TS), and 85.50% in total suspended solids (TSS) in the treated textile effluent. The non-toxic nature of <i>Escherichia</i> sp. PP001-treated dyes and textile effluent were confirmed through phytotoxicity studies. Consequently, the treated effluent can be recommended for irrigation.</p><h3>Graphical Abstract</h3>\n<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":808,"journal":{"name":"Water, Air, & Soil Pollution","volume":"236 14","pages":""},"PeriodicalIF":3.0000,"publicationDate":"2025-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Enhanced Bacterial Degradation of Azo dyes by Escherichia sp. PP001: Optimization, Kinetics, Degradation Pathway, Phytotoxicity and Textile Wastewater Treatment\",\"authors\":\"Mohanasundram Sudha, Arul Ponniah Saranya, Ganeshamoorthy Bakiyaraj, Mariappan Mariappan, Natesan Sivakumar\",\"doi\":\"10.1007/s11270-025-08529-3\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Azo dyes are organic, artificial colorants with a wide range of applications as textile coloring agents. The disposal of textile effluent negatively impacts soil and water ecosystems; hence, enhancing the microbial degradation of azo dyes is essential for the textile industry's disposal treatment. The prime focus of this research is studying the bacterial degradation of azo textile dyes, Direct Green 28 (DG-28) and Direct Blue 71 (DB-71). Taxonomic studies of the isolated strain revealed that it was <i>Escherichia</i> sp. PP001 (GenBank: KP770133.1), and it has the potential to degrade azo dyes. The culture conditions were optimized under various physiological parameters such as dye concentrations, pH, temperature, and agitation. The maximum decolorization of 99% was achieved for both dyes at pH 7, 37 °C, with agitation of 60 rpm (revolutions per minute). Biosorption kinetics fit pseudo-zero-order and diffusion models, indicating surface adsorption and intraparticle diffusion. GC–MS (Gas Chromatography Mass Spectroscopy) analysis elucidated metabolite degradation pathways. Physicochemical properties of the treated and untreated textile effluent were examined and compared. The results indicate a reduction of 97.63% in biological oxygen demand (BOD), 96.23% in chemical oxygen demand (COD), 64.56% in total dissolved solids (TDS), 76.80% in total solids (TS), and 85.50% in total suspended solids (TSS) in the treated textile effluent. The non-toxic nature of <i>Escherichia</i> sp. PP001-treated dyes and textile effluent were confirmed through phytotoxicity studies. Consequently, the treated effluent can be recommended for irrigation.</p><h3>Graphical Abstract</h3>\\n<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>\",\"PeriodicalId\":808,\"journal\":{\"name\":\"Water, Air, & Soil Pollution\",\"volume\":\"236 14\",\"pages\":\"\"},\"PeriodicalIF\":3.0000,\"publicationDate\":\"2025-10-04\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Water, Air, & Soil Pollution\",\"FirstCategoryId\":\"6\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s11270-025-08529-3\",\"RegionNum\":4,\"RegionCategory\":\"环境科学与生态学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"ENVIRONMENTAL SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Water, Air, & Soil Pollution","FirstCategoryId":"6","ListUrlMain":"https://link.springer.com/article/10.1007/s11270-025-08529-3","RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ENVIRONMENTAL SCIENCES","Score":null,"Total":0}
Enhanced Bacterial Degradation of Azo dyes by Escherichia sp. PP001: Optimization, Kinetics, Degradation Pathway, Phytotoxicity and Textile Wastewater Treatment
Azo dyes are organic, artificial colorants with a wide range of applications as textile coloring agents. The disposal of textile effluent negatively impacts soil and water ecosystems; hence, enhancing the microbial degradation of azo dyes is essential for the textile industry's disposal treatment. The prime focus of this research is studying the bacterial degradation of azo textile dyes, Direct Green 28 (DG-28) and Direct Blue 71 (DB-71). Taxonomic studies of the isolated strain revealed that it was Escherichia sp. PP001 (GenBank: KP770133.1), and it has the potential to degrade azo dyes. The culture conditions were optimized under various physiological parameters such as dye concentrations, pH, temperature, and agitation. The maximum decolorization of 99% was achieved for both dyes at pH 7, 37 °C, with agitation of 60 rpm (revolutions per minute). Biosorption kinetics fit pseudo-zero-order and diffusion models, indicating surface adsorption and intraparticle diffusion. GC–MS (Gas Chromatography Mass Spectroscopy) analysis elucidated metabolite degradation pathways. Physicochemical properties of the treated and untreated textile effluent were examined and compared. The results indicate a reduction of 97.63% in biological oxygen demand (BOD), 96.23% in chemical oxygen demand (COD), 64.56% in total dissolved solids (TDS), 76.80% in total solids (TS), and 85.50% in total suspended solids (TSS) in the treated textile effluent. The non-toxic nature of Escherichia sp. PP001-treated dyes and textile effluent were confirmed through phytotoxicity studies. Consequently, the treated effluent can be recommended for irrigation.
期刊介绍：
Water, Air, & Soil Pollution is an international, interdisciplinary journal on all aspects of pollution and solutions to pollution in the biosphere. This includes chemical, physical and biological processes affecting flora, fauna, water, air and soil in relation to environmental pollution. Because of its scope, the subject areas are diverse and include all aspects of pollution sources, transport, deposition, accumulation, acid precipitation, atmospheric pollution, metals, aquatic pollution including marine pollution and ground water, waste water, pesticides, soil pollution, sewage, sediment pollution, forestry pollution, effects of pollutants on humans, vegetation, fish, aquatic species, micro-organisms, and animals, environmental and molecular toxicology applied to pollution research, biosensors, global and climate change, ecological implications of pollution and pollution models. Water, Air, & Soil Pollution also publishes manuscripts on novel methods used in the study of environmental pollutants, environmental toxicology, environmental biology, novel environmental engineering related to pollution, biodiversity as influenced by pollution, novel environmental biotechnology as applied to pollution (e.g. bioremediation), environmental modelling and biorestoration of polluted environments.
Articles should not be submitted that are of local interest only and do not advance international knowledge in environmental pollution and solutions to pollution. Articles that simply replicate known knowledge or techniques while researching a local pollution problem will normally be rejected without review. Submitted articles must have up-to-date references, employ the correct experimental replication and statistical analysis, where needed and contain a significant contribution to new knowledge. The publishing and editorial team sincerely appreciate your cooperation.
Water, Air, & Soil Pollution publishes research papers; review articles; mini-reviews; and book reviews.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
