Canye Chen, Tianqi Liu, Xi Luo, Lizhen Yang, Dan He, Wenbing Zhou, Jianbo Cai
{"title":"水力停留时间和碳氮比对厌氧处理猪废水中抗生素耐药基因和微生物群落的影响","authors":"Canye Chen, Tianqi Liu, Xi Luo, Lizhen Yang, Dan He, Wenbing Zhou, Jianbo Cai","doi":"10.1007/s11270-025-08486-x","DOIUrl":null,"url":null,"abstract":"<div><p>In swine wastewater treatment, it is important to regulate the technical parameters to control the antimicrobial resistance genes and microbial community in the anaerobic treatment system. In this study, a continuous stirred tank reactor (CSTR) was used to investigate the changes in five tetracycline resistance genes, two mobile genetic elements, and microbial community composition in the system with different parameter settings (hydraulic retention time, HRT) and carbon to nitrogen ratio (C/N ratio) for anaerobic treatment of swine wastewater. Redundancy analysis identified Proteus and bacteroides as the potential hosts for the <i>tetA, tetM, tetO</i>, and <i>Tn916/1545</i> genes, spirochetes as the potential host for the <i>tetX</i> gene, and Firmicutes as the potential hosts for the <i>tetG</i> and <i>intI1</i> genes. The relative abundance of the <i>tetA</i>, <i>tetM</i>, and <i>tetO</i> genes experienced a significant reduction of 75.16%, 63.91%, and 44.07%, respectively, as the HRT was extended from 18 to 48 h. The relative abundance of the <i>tetA, tetG, tetM</i>, and <i>tetO</i> genes, along with the <i>Intl1</i> gene, increased accordingly in the C/N ratio test group with the C/N ratio gradually increasing from 12.5 to 20, which significantly exceeded that of the control group. In summary, this study assessed the effects of treatment conditions, specifically HRT and C/N ratio, on the antibiotic resistance in swine wastewater, and the findings provide valuable implications for the design of effective wastewater treatment systems in swine farms.</p><h3>Graphical Abstract</h3>\n<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":808,"journal":{"name":"Water, Air, & Soil Pollution","volume":"236 13","pages":""},"PeriodicalIF":3.0000,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Influence of Hydraulic Retention Time and C/N Ratio on Antibiotic Resistance Genes and Microbial Communities in Anaerobic Treatment of Swine Wastewater\",\"authors\":\"Canye Chen, Tianqi Liu, Xi Luo, Lizhen Yang, Dan He, Wenbing Zhou, Jianbo Cai\",\"doi\":\"10.1007/s11270-025-08486-x\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>In swine wastewater treatment, it is important to regulate the technical parameters to control the antimicrobial resistance genes and microbial community in the anaerobic treatment system. In this study, a continuous stirred tank reactor (CSTR) was used to investigate the changes in five tetracycline resistance genes, two mobile genetic elements, and microbial community composition in the system with different parameter settings (hydraulic retention time, HRT) and carbon to nitrogen ratio (C/N ratio) for anaerobic treatment of swine wastewater. Redundancy analysis identified Proteus and bacteroides as the potential hosts for the <i>tetA, tetM, tetO</i>, and <i>Tn916/1545</i> genes, spirochetes as the potential host for the <i>tetX</i> gene, and Firmicutes as the potential hosts for the <i>tetG</i> and <i>intI1</i> genes. The relative abundance of the <i>tetA</i>, <i>tetM</i>, and <i>tetO</i> genes experienced a significant reduction of 75.16%, 63.91%, and 44.07%, respectively, as the HRT was extended from 18 to 48 h. The relative abundance of the <i>tetA, tetG, tetM</i>, and <i>tetO</i> genes, along with the <i>Intl1</i> gene, increased accordingly in the C/N ratio test group with the C/N ratio gradually increasing from 12.5 to 20, which significantly exceeded that of the control group. 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Influence of Hydraulic Retention Time and C/N Ratio on Antibiotic Resistance Genes and Microbial Communities in Anaerobic Treatment of Swine Wastewater
In swine wastewater treatment, it is important to regulate the technical parameters to control the antimicrobial resistance genes and microbial community in the anaerobic treatment system. In this study, a continuous stirred tank reactor (CSTR) was used to investigate the changes in five tetracycline resistance genes, two mobile genetic elements, and microbial community composition in the system with different parameter settings (hydraulic retention time, HRT) and carbon to nitrogen ratio (C/N ratio) for anaerobic treatment of swine wastewater. Redundancy analysis identified Proteus and bacteroides as the potential hosts for the tetA, tetM, tetO, and Tn916/1545 genes, spirochetes as the potential host for the tetX gene, and Firmicutes as the potential hosts for the tetG and intI1 genes. The relative abundance of the tetA, tetM, and tetO genes experienced a significant reduction of 75.16%, 63.91%, and 44.07%, respectively, as the HRT was extended from 18 to 48 h. The relative abundance of the tetA, tetG, tetM, and tetO genes, along with the Intl1 gene, increased accordingly in the C/N ratio test group with the C/N ratio gradually increasing from 12.5 to 20, which significantly exceeded that of the control group. In summary, this study assessed the effects of treatment conditions, specifically HRT and C/N ratio, on the antibiotic resistance in swine wastewater, and the findings provide valuable implications for the design of effective wastewater treatment systems in swine farms.
期刊介绍：
Water, Air, & Soil Pollution is an international, interdisciplinary journal on all aspects of pollution and solutions to pollution in the biosphere. This includes chemical, physical and biological processes affecting flora, fauna, water, air and soil in relation to environmental pollution. Because of its scope, the subject areas are diverse and include all aspects of pollution sources, transport, deposition, accumulation, acid precipitation, atmospheric pollution, metals, aquatic pollution including marine pollution and ground water, waste water, pesticides, soil pollution, sewage, sediment pollution, forestry pollution, effects of pollutants on humans, vegetation, fish, aquatic species, micro-organisms, and animals, environmental and molecular toxicology applied to pollution research, biosensors, global and climate change, ecological implications of pollution and pollution models. Water, Air, & Soil Pollution also publishes manuscripts on novel methods used in the study of environmental pollutants, environmental toxicology, environmental biology, novel environmental engineering related to pollution, biodiversity as influenced by pollution, novel environmental biotechnology as applied to pollution (e.g. bioremediation), environmental modelling and biorestoration of polluted environments.
Articles should not be submitted that are of local interest only and do not advance international knowledge in environmental pollution and solutions to pollution. Articles that simply replicate known knowledge or techniques while researching a local pollution problem will normally be rejected without review. Submitted articles must have up-to-date references, employ the correct experimental replication and statistical analysis, where needed and contain a significant contribution to new knowledge. The publishing and editorial team sincerely appreciate your cooperation.
Water, Air, & Soil Pollution publishes research papers; review articles; mini-reviews; and book reviews.
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