微流体装置类型改善心脏mRNA在体内的传递。

IF 3.9 2区化学 Q2 CHEMISTRY, MULTIDISCIPLINARY

Langmuir Pub Date : 2025-10-02 DOI:10.1021/acs.langmuir.5c02612

Elisa Schrader Echeverri,Hyejin Kim,Bora Jang,Avraham Shakked,Christian Park,Kyung In Baek,Leandro Choi,Dong Won Kang,Ruei-Chun Hung,Kiyoung Jeong,Hannah E Peck,Ananda R Podilapu,Karen E Tiegreen,Philip J Santangelo,Hanjoong Jo,James E Dahlman

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引用次数: 0

摘要

为了改善脂质纳米颗粒（LNP）介导的非肝脏组织递送，科学家们修改了LNP的化学性质或添加了靶向配体。一个未被充分探索的替代方案是改变创造LNP的配方过程。在这里，我们报告了用人字形混合器配制的LNP比用分岔混合器配制的LNP的心脏交付量多2倍。这两种LNPs具有相似的生物物理特性，但它们吸附的蛋白质冠状体不同，这表明这种效应是由系统生理驱动的。通过使用空间转录组学跟踪LNP在动脉粥样硬化小鼠模型中的传递，我们发现改良的LNP在静脉注射后将mRNA传递到病变区域和心肌细胞。这些数据表明，通过纳米颗粒处理增加心脏输送是可能的。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Microfluidic Device Type Improves Heart mRNA Delivery In Vivo.

To improve lipid nanoparticle (LNP)-mediated delivery to nonliver tissues, scientists modify LNP chemistry or add targeting ligands. One underexplored alternative is to change the formulation process that creates the LNP. Here, we report that an LNP formulated with a herringbone mixer led to 2-fold more heart delivery than the same LNP formulated with a bifurcating mixer. The two LNPs had similar biophysical traits, yet they adsorbed different protein coronas, suggesting that the effect was driven by systemic physiology. By using spatial transcriptomics to track LNP delivery in a mouse model of atherosclerosis, we found that the improved LNP delivered mRNA to diseased regions and cardiomyocytes after an intravenous injection. These data suggest that it is possible to increase heart delivery via nanoparticle processing.

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来源期刊

Langmuir 化学-材料科学：综合

CiteScore

6.50

自引率

10.30%

发文量

1464

审稿时长

2.1 months

期刊介绍： Langmuir is an interdisciplinary journal publishing articles in the following subject categories: Colloids: surfactants and self-assembly, dispersions, emulsions, foams Interfaces: adsorption, reactions, films, forces Biological Interfaces: biocolloids, biomolecular and biomimetic materials Materials: nano- and mesostructured materials, polymers, gels, liquid crystals Electrochemistry: interfacial charge transfer, charge transport, electrocatalysis, electrokinetic phenomena, bioelectrochemistry Devices and Applications: sensors, fluidics, patterning, catalysis, photonic crystals However, when high-impact, original work is submitted that does not fit within the above categories, decisions to accept or decline such papers will be based on one criteria: What Would Irving Do? Langmuir ranks #2 in citations out of 136 journals in the category of Physical Chemistry with 113,157 total citations. The journal received an Impact Factor of 4.384*. This journal is also indexed in the categories of Materials Science (ranked #1) and Multidisciplinary Chemistry (ranked #5).