用粒子诱导x射线发射分析羊眼泪的元素组成。

IF 1.3 4区农林科学 Q2 VETERINARY SCIENCES

Veterinary ophthalmology Pub Date : 2025-10-01 DOI:10.1111/vop.70096

Alon Zahavi, Oren Pe'er, Noa Cohen Sinai, Yoav Vardizer, Lionel Sebbag, Ron Ofri, Olga Girshevitz, Nitza Goldenberg-Cohen

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引用次数: 0

摘要

目的：利用粒子诱导x射线发射（PIXE）技术鉴定羊泪液的元素组成。实验动物：10只身体健康的母羊。步骤：使用Schirmer撕裂试纸收集撕裂样本。连续5天每天收集一次泪液样本以评估再现性。用pixie进行元素分析。数据用GUPIX软件处理。用Kruskal-Wallis检验和事后Dunn检验计算鉴定元素的中位数并进行统计分析。结果：分析表明，在5天的采样期间，元素浓度一致。在检测到的元素中，氯（Cl）和钠（Na）的浓度最高。结论：本研究建立了一种有价值的检测和分析绵羊泪膜元素组成的技术。通过提供一种精确的小生物样本元素分析方法，该方法为未来的研究奠定了基础，探索其在泪液分析中的可靠性和敏感性，以及作为眼部和全身健康生物标志物的潜力。应该研究年龄、饮食和环境暴露等变量对泪膜成分的影响，可能会将该方法扩展到其他物种。

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Elemental Composition Analysis of Sheep Tears Using Particle-Induced X-Ray Emission.

Objective: To identify the elemental composition of ovine tears using particle-induced X-ray emission (PIXE).

Animals studied: Ten physically healthy female sheep.

Procedures: Tear samples were collected using Schirmer tear test strips. Tear samples were collected once daily over five consecutive days to assess reproducibility. Elemental analysis was performed with PIXE. Data were processed with GUPIX software. Median values of the elements identified were calculated and statistically analyzed with Kruskal-Wallis and post hoc Dunn's tests.

Results: The analysis demonstrated consistent elemental concentrations over the 5-day sampling period. Among the elements detected, chloride (Cl) and sodium (Na) exhibited the highest concentrations.

Conclusions: This study establishes PIXE as a valuable technique for detecting and analyzing the elemental composition of the tear film in sheep. By providing a precise method for elemental analysis in small biological samples, this approach lays the groundwork for future research exploring its reliability and sensitivity in tear analysis and potential as a biomarker for ocular and systemic health. The impact of variables including age, diet, and environmental exposure on tear film constituents should be studied, potentially extending the methodology to other species.

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来源期刊

Veterinary ophthalmology 农林科学-兽医学

CiteScore

2.70

自引率

37.50%

发文量

审稿时长

12-24 weeks

期刊介绍： Veterinary Ophthalmology is a peer-reviewed, international journal that welcomes submission of manuscripts directed towards academic researchers of veterinary ophthalmology, specialists and general practitioners with a strong ophthalmology interest. Articles include those relating to all aspects of: Clinical and investigational veterinary and comparative ophthalmology; Prospective and retrospective studies or reviews of naturally occurring ocular disease in veterinary species; Experimental models of both animal and human ocular disease in veterinary species; Anatomic studies of the animal eye; Physiological studies of the animal eye; Pharmacological studies of the animal eye.