Kexin Li, Tai Li, Yonglong Liu, Bingchen Zou, Gui Geng, Yao Xu, Jiahui Liu, Yuguang Wang
{"title":"解码根际协同作用:假单胞菌和芽孢杆菌增强微生物介导的甜菜根核孢子菌抑制。","authors":"Kexin Li, Tai Li, Yonglong Liu, Bingchen Zou, Gui Geng, Yao Xu, Jiahui Liu, Yuguang Wang","doi":"10.1094/PHYTO-05-25-0159-R","DOIUrl":null,"url":null,"abstract":"<p><p>Sugar beet is a crucial sugar crop with substantial economic and nutritional value. The occurrence of damping-off disease severely impacts sugar beet quality and yield. Here, we successfully isolated two endophytes from sugar beet, and it follow as <i>Bacillus albus</i> SB-3 and <i>Pseudomonas chlororaphis</i> SB-35, via morphological observation and molecular identification. Both SB-3 and SB-35 exhibited nitrogen-fixing and potassium mobilization capabilities, with SB-35 demonstrating additional traits including phosphate solubilization, potassium mobilization. SB-3 and SB-35 promoted the growth of sugar beet, resulting in increased biomass, and improved soil available nutrient. Besides, SB-3 and SB-35 had also extracellular protease activities and inhibited the mycelium growth of <i>Rhizoctonia solani</i>. In independent pot experiments, SB-3 and SB-35 exhibited significantly controlling the damping-off of seedlings for sugar beet. Further analysis indicated that SB-3 and SB-35 may alter microbial community structure, reducing the abundance of <i>Rhizoctonia solani</i>, promoting the recruitment of beneficial microorganisms, such as <i>Hypocrea, Peziza</i>, and <i>Talaromyces</i>, to occupy ecological niches, thereby reducing the numbers of pathogen. The two bacterial strains modulated the diversity and community structure of rhizosphere microorganisms, suggesting a microbiome-mediated mechanism underlying their host-beneficial effects. This study advances our understanding of harnessing endophytes to enhance sugar beet productivity and suppressing sugar beet damping-off caused by <i>Rhizoctonia solani</i>.</p>","PeriodicalId":20410,"journal":{"name":"Phytopathology","volume":" ","pages":""},"PeriodicalIF":3.1000,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Decoding Rhizosphere Synergies: <i>Pseudomonas</i> and <i>Bacillus</i> Enhance Microbiome-Mediated Suppression of <i>Rhizoctonia solani</i> in Sugar Beet.\",\"authors\":\"Kexin Li, Tai Li, Yonglong Liu, Bingchen Zou, Gui Geng, Yao Xu, Jiahui Liu, Yuguang Wang\",\"doi\":\"10.1094/PHYTO-05-25-0159-R\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Sugar beet is a crucial sugar crop with substantial economic and nutritional value. The occurrence of damping-off disease severely impacts sugar beet quality and yield. Here, we successfully isolated two endophytes from sugar beet, and it follow as <i>Bacillus albus</i> SB-3 and <i>Pseudomonas chlororaphis</i> SB-35, via morphological observation and molecular identification. Both SB-3 and SB-35 exhibited nitrogen-fixing and potassium mobilization capabilities, with SB-35 demonstrating additional traits including phosphate solubilization, potassium mobilization. SB-3 and SB-35 promoted the growth of sugar beet, resulting in increased biomass, and improved soil available nutrient. Besides, SB-3 and SB-35 had also extracellular protease activities and inhibited the mycelium growth of <i>Rhizoctonia solani</i>. In independent pot experiments, SB-3 and SB-35 exhibited significantly controlling the damping-off of seedlings for sugar beet. Further analysis indicated that SB-3 and SB-35 may alter microbial community structure, reducing the abundance of <i>Rhizoctonia solani</i>, promoting the recruitment of beneficial microorganisms, such as <i>Hypocrea, Peziza</i>, and <i>Talaromyces</i>, to occupy ecological niches, thereby reducing the numbers of pathogen. The two bacterial strains modulated the diversity and community structure of rhizosphere microorganisms, suggesting a microbiome-mediated mechanism underlying their host-beneficial effects. This study advances our understanding of harnessing endophytes to enhance sugar beet productivity and suppressing sugar beet damping-off caused by <i>Rhizoctonia solani</i>.</p>\",\"PeriodicalId\":20410,\"journal\":{\"name\":\"Phytopathology\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":3.1000,\"publicationDate\":\"2025-10-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Phytopathology\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1094/PHYTO-05-25-0159-R\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Phytopathology","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1094/PHYTO-05-25-0159-R","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
Decoding Rhizosphere Synergies: Pseudomonas and Bacillus Enhance Microbiome-Mediated Suppression of Rhizoctonia solani in Sugar Beet.
Sugar beet is a crucial sugar crop with substantial economic and nutritional value. The occurrence of damping-off disease severely impacts sugar beet quality and yield. Here, we successfully isolated two endophytes from sugar beet, and it follow as Bacillus albus SB-3 and Pseudomonas chlororaphis SB-35, via morphological observation and molecular identification. Both SB-3 and SB-35 exhibited nitrogen-fixing and potassium mobilization capabilities, with SB-35 demonstrating additional traits including phosphate solubilization, potassium mobilization. SB-3 and SB-35 promoted the growth of sugar beet, resulting in increased biomass, and improved soil available nutrient. Besides, SB-3 and SB-35 had also extracellular protease activities and inhibited the mycelium growth of Rhizoctonia solani. In independent pot experiments, SB-3 and SB-35 exhibited significantly controlling the damping-off of seedlings for sugar beet. Further analysis indicated that SB-3 and SB-35 may alter microbial community structure, reducing the abundance of Rhizoctonia solani, promoting the recruitment of beneficial microorganisms, such as Hypocrea, Peziza, and Talaromyces, to occupy ecological niches, thereby reducing the numbers of pathogen. The two bacterial strains modulated the diversity and community structure of rhizosphere microorganisms, suggesting a microbiome-mediated mechanism underlying their host-beneficial effects. This study advances our understanding of harnessing endophytes to enhance sugar beet productivity and suppressing sugar beet damping-off caused by Rhizoctonia solani.
期刊介绍:
Phytopathology publishes articles on fundamental research that advances understanding of the nature of plant diseases, the agents that cause them, their spread, the losses they cause, and measures that can be used to control them. Phytopathology considers manuscripts covering all aspects of plant diseases including bacteriology, host-parasite biochemistry and cell biology, biological control, disease control and pest management, description of new pathogen species description of new pathogen species, ecology and population biology, epidemiology, disease etiology, host genetics and resistance, mycology, nematology, plant stress and abiotic disorders, postharvest pathology and mycotoxins, and virology. Papers dealing mainly with taxonomy, such as descriptions of new plant pathogen taxa are acceptable if they include plant disease research results such as pathogenicity, host range, etc. Taxonomic papers that focus on classification, identification, and nomenclature below the subspecies level may also be submitted to Phytopathology.