柑橘黄单胞菌新基因组。来自现代和历史材料的幼虫揭示了一种被忽视的作物病原体的历史和基因组学。

IF 3.1 2区 农林科学 Q2 PLANT SCIENCES
Claudine Boyer, Paola Campos, Nathalie Becker, Lionel Gagnevin, Karine Boyer, Timothy M A Utteridge, Olivier Pruvost, Adrien Rieux
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引用次数: 0

摘要

在这项研究中,我们提出了新的柑橘黄单胞菌的基因组数据。百耳树细菌性空穴病的病原菌bilvae (Xcb)。使用结合短读和长读技术的混合测序方法,我们组装了仅有的两种可用的当代Xcb菌株的高质量基因组。此外,我们从1848年收集的植物标本中重建了Xcb的第一个历史基因组,从而将这种被忽视的疾病在印度的存在时间延长了近100年。然后,我们利用一套专门的生物信息学工具,对这些菌株的基因组特征进行了表征,特别强调了毒力因子和质粒含量。发现当代Xcb菌株携带1至4个质粒,其迁移潜力(共轭,可移动或不可移动)各不相同。在染色体和质粒中共鉴定出30 ~ 32个III型效应基因。值得注意的是,其中一个当代菌株含有四个质粒携带的转录激活因子样效应物(TALEs),与X. citri pv中的TALEs只有遥远的相似性。柑橘,一种全球主要病原体,宿主范围部分重叠。当代和历史菌株的基因组比较分析显示,效应基因含量具有显著的保守性,表明关键的致病性状可能在Xcb的进化史早期就获得了。总的来说,这些新的基因组资源为这种未被开发的细菌病原体的生物学和进化提供了有价值的见解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
New Genomes of Xanthomonas citri pv. bilvae from Modern and Historical Material Reveal the History and Genomics of a Neglected Crop Pathogen.

In this study, we present novel genomic data for Xanthomonas citri pv. bilvae (Xcb), the causal agent of bacterial shot-hole disease in bael trees. Using a hybrid sequencing approach that combines short- and long-read technologies, we assembled high-quality genomes of the only two available contemporary Xcb strains. Furthermore, we reconstructed the first historical genome of Xcb from a herbarium specimen collected in 1848, thereby extending the documented presence of this overlooked disease in India by nearly 100 years. We then characterized the genomic features of these strains, with a particular emphasis on virulence factors and plasmid content, using a suite of specialized bioinformatics tools. The contemporary Xcb strains were found to carry between one and four plasmids, which varied in their mobility potential (conjugative, mobilizable, or non-mobile). A total of 30 to 32 type III effector (T3E) genes were identified across chromosomes and plasmids. Notably, one of the contemporary strains harbored four plasmid-borne transcription activator-like effectors (TALEs), which showed only distant similarity to TALEs found in X. citri pv. citri, a globally major pathogen with a partially overlapping host range. Comparative genomic analysis between the contemporary and historical strains revealed a remarkable conservation of effector gene content, indicating that key pathogenic traits may have been acquired early in Xcb's evolutionary history. Collectively, these new genomic resources provide valuable insights into the biology and evolution of this underexplored bacterial pathogen.

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来源期刊
Phytopathology
Phytopathology 生物-植物科学
CiteScore
5.90
自引率
9.40%
发文量
505
审稿时长
4-8 weeks
期刊介绍: Phytopathology publishes articles on fundamental research that advances understanding of the nature of plant diseases, the agents that cause them, their spread, the losses they cause, and measures that can be used to control them. Phytopathology considers manuscripts covering all aspects of plant diseases including bacteriology, host-parasite biochemistry and cell biology, biological control, disease control and pest management, description of new pathogen species description of new pathogen species, ecology and population biology, epidemiology, disease etiology, host genetics and resistance, mycology, nematology, plant stress and abiotic disorders, postharvest pathology and mycotoxins, and virology. Papers dealing mainly with taxonomy, such as descriptions of new plant pathogen taxa are acceptable if they include plant disease research results such as pathogenicity, host range, etc. Taxonomic papers that focus on classification, identification, and nomenclature below the subspecies level may also be submitted to Phytopathology.
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