Jin Wang , Md Abu Kawocha , Tengfei Liu , Tiantian Liu , Jingjing Guo , Shulan Hu , Yi Liu , Shengxuan Liu , Lin Chen , Bihua Nie , Botao Song
{"title":"tono质体糖转运蛋白StTST1介导马铃薯液泡糖分配和非生物胁迫抗性。","authors":"Jin Wang , Md Abu Kawocha , Tengfei Liu , Tiantian Liu , Jingjing Guo , Shulan Hu , Yi Liu , Shengxuan Liu , Lin Chen , Bihua Nie , Botao Song","doi":"10.1016/j.plaphy.2025.110549","DOIUrl":null,"url":null,"abstract":"<div><div>Sugar transporters play pivotal roles in plant growth, development, and stress responses. However, the function of sugar transporters in potato (Solanum tuberosum) is still obscure. In this study, the function of potato tonoplast sugar transporter 1 (StTST1) in subcellular sugar compartmentation and abiotic stress tolerance was characterized. Heterologous expression assays in <em>Saccharomyces cerevisiae</em> (strain W303) demonstrated that StTST1 mediates sucrose transporting into the vacuole. Moreover overexpression and RNA interference (RNAi) of <em>StTST1</em> in potato altered leafy sugar content including sucrose, glucose, and fructose without affecting the activity of key metabolic enzymes. Intriguingly, RNAi-mediated suppression of <em>StTST1</em> enhanced freezing tolerance and compromised drought tolerance. In contrast, overexpression of StTST1 enhanced drought tolerance but reduced freezing tolerance. Our results demonstrate that StTST1 dynamically regulates subcellular sugar partitioning and differentially modulates freezing and drought stress responses. These findings highlight the potential of targeted manipulation of sugar transporters to modulate crop resilience to multiple abiotic stresses.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"229 ","pages":"Article 110549"},"PeriodicalIF":5.7000,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Tonoplast sugar transporter StTST1 mediates vacuolar sugar partitioning and abiotic stress tolerance in potato\",\"authors\":\"Jin Wang , Md Abu Kawocha , Tengfei Liu , Tiantian Liu , Jingjing Guo , Shulan Hu , Yi Liu , Shengxuan Liu , Lin Chen , Bihua Nie , Botao Song\",\"doi\":\"10.1016/j.plaphy.2025.110549\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Sugar transporters play pivotal roles in plant growth, development, and stress responses. However, the function of sugar transporters in potato (Solanum tuberosum) is still obscure. In this study, the function of potato tonoplast sugar transporter 1 (StTST1) in subcellular sugar compartmentation and abiotic stress tolerance was characterized. Heterologous expression assays in <em>Saccharomyces cerevisiae</em> (strain W303) demonstrated that StTST1 mediates sucrose transporting into the vacuole. Moreover overexpression and RNA interference (RNAi) of <em>StTST1</em> in potato altered leafy sugar content including sucrose, glucose, and fructose without affecting the activity of key metabolic enzymes. Intriguingly, RNAi-mediated suppression of <em>StTST1</em> enhanced freezing tolerance and compromised drought tolerance. In contrast, overexpression of StTST1 enhanced drought tolerance but reduced freezing tolerance. Our results demonstrate that StTST1 dynamically regulates subcellular sugar partitioning and differentially modulates freezing and drought stress responses. These findings highlight the potential of targeted manipulation of sugar transporters to modulate crop resilience to multiple abiotic stresses.</div></div>\",\"PeriodicalId\":20234,\"journal\":{\"name\":\"Plant Physiology and Biochemistry\",\"volume\":\"229 \",\"pages\":\"Article 110549\"},\"PeriodicalIF\":5.7000,\"publicationDate\":\"2025-09-25\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Plant Physiology and Biochemistry\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0981942825010770\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant Physiology and Biochemistry","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0981942825010770","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
Tonoplast sugar transporter StTST1 mediates vacuolar sugar partitioning and abiotic stress tolerance in potato
Sugar transporters play pivotal roles in plant growth, development, and stress responses. However, the function of sugar transporters in potato (Solanum tuberosum) is still obscure. In this study, the function of potato tonoplast sugar transporter 1 (StTST1) in subcellular sugar compartmentation and abiotic stress tolerance was characterized. Heterologous expression assays in Saccharomyces cerevisiae (strain W303) demonstrated that StTST1 mediates sucrose transporting into the vacuole. Moreover overexpression and RNA interference (RNAi) of StTST1 in potato altered leafy sugar content including sucrose, glucose, and fructose without affecting the activity of key metabolic enzymes. Intriguingly, RNAi-mediated suppression of StTST1 enhanced freezing tolerance and compromised drought tolerance. In contrast, overexpression of StTST1 enhanced drought tolerance but reduced freezing tolerance. Our results demonstrate that StTST1 dynamically regulates subcellular sugar partitioning and differentially modulates freezing and drought stress responses. These findings highlight the potential of targeted manipulation of sugar transporters to modulate crop resilience to multiple abiotic stresses.
期刊介绍:
Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement.
Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB.
Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.