拟南芥GyraseB3通过促进组蛋白去乙酰化参与转座子沉默。

IF 13.1 2区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Isabelle Gy, Sébastien Beaubiat, Nicolas Bouché
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引用次数: 0

摘要

DNA甲基化和组蛋白修饰是控制染色质结构、基因表达和转座因子(TE)活性的关键表观遗传标记。在植物中,盆景甲基化1 (IBM1)组蛋白去甲基化酶的增加阻止了异色沉默标记在活性转录基因上的积累。通过对IBM1产生缺陷突变体的遗传筛选,我们发现了维持全基因组表观遗传状态平衡所必需的基因的抑制性突变。gyrb3突变部分逆转了ibm1缺陷植物的DNA超甲基化,揭示了gyrb3的新作用,gyrb3是一种核蛋白,结合了蓝藻gyrase和ELM2/SANT蛋白的结构域,参与组蛋白乙酰化。在gyrb3突变体中,TEs表现出转录活性,表现出DNA甲基化减少和组蛋白H3乙酰化增加,这两者都是与表达激活相关的表观遗传标记。GyrB3与组蛋白去乙酰化酶如histone DEACETYLASE6 (HDA6)发生物理相互作用,可能介导其在TEs中的活性。HDA6和GyrB3之间的功能重叠进一步得到了观察结果的支持,与GyrB3相似,HDA6的突变抑制了Ibm2表型。我们的研究结果表明,组蛋白去乙酰化对TE沉默至关重要,IBM1在植物中的缺失消除了基因和TE之间的边界,强调了其在维持表观基因组稳定性方面的重要性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The arabidopsis GyraseB3 contributes to transposon silencing by promoting histone deacetylation.

DNA methylation and histone modifications are key epigenetic marks controlling chromatin structure, gene expression, and transposable element (TE) activity. In plants, the histone demethylase INCREASE IN BONSAI METHYLATION1 (IBM1) prevents heterochromatic silencing marks from accumulating on actively transcribed genes. Through a genetic screen of mutants defective in IBM1 production, we identified suppressor mutations in genes essential for maintaining balanced genome-wide epigenetic states. The gyrb3 mutation partly reversed DNA hypermethylation in IBM1-deficient plants, revealing a novel role for GyrB3, a nuclear protein combining domains from cyanobacterial gyrases and ELM2/SANT proteins involved in histone acetylation. In gyrb3 mutants, TEs exhibit transcriptional activity, showing reduced DNA methylation and increased histone H3 acetylation, both of which are epigenetic marks associated with expression activation. GyrB3 physically interacts with histone deacetylases like HISTONE DEACETYLASE6 (HDA6), likely mediating their activities at TEs. The functional overlap between HDA6 and GyrB3 is further supported by the observation that, similar to gyrb3, a mutation in hda6 suppresses the Ibm2 phenotype. Our findings reinforce that histone deacetylation is essential for TE silencing and that loss of IBM1 in plants abolished the frontiers between genes and TEs, emphasizing its importance in maintaining epigenomic stability.

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来源期刊
Nucleic Acids Research
Nucleic Acids Research 生物-生化与分子生物学
CiteScore
27.10
自引率
4.70%
发文量
1057
审稿时长
2 months
期刊介绍: Nucleic Acids Research (NAR) is a scientific journal that publishes research on various aspects of nucleic acids and proteins involved in nucleic acid metabolism and interactions. It covers areas such as chemistry and synthetic biology, computational biology, gene regulation, chromatin and epigenetics, genome integrity, repair and replication, genomics, molecular biology, nucleic acid enzymes, RNA, and structural biology. The journal also includes a Survey and Summary section for brief reviews. Additionally, each year, the first issue is dedicated to biological databases, and an issue in July focuses on web-based software resources for the biological community. Nucleic Acids Research is indexed by several services including Abstracts on Hygiene and Communicable Diseases, Animal Breeding Abstracts, Agricultural Engineering Abstracts, Agbiotech News and Information, BIOSIS Previews, CAB Abstracts, and EMBASE.
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