Tesha Kerr Carpenter, Mariah McCashland, Raechel Sherrick, Sathish Kumar Natarajan, Mirko Mandić, Stephen D Kachman, Ferdinand von Walden, Ivan J Vechetti, Rodrigo Fernandez-Gonzalo
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引用次数: 0
摘要
目的：脂质是细胞外囊泡（EVs）中最丰富的分子之一，对细胞外囊泡生物学和细胞信号传导具有重要意义。然而，很少有人注意到脂质在运动过程中ev的生物学功能中的作用。因此，我们使用交叉设计完成了一项研究，以调查有氧和阻力运动对ev脂质谱的影响。方法：10名健康受试者（23.0±3.6岁）采用随机交叉设计，进行急性有氧运动、急性阻力运动和一段时间休息（对照组）。在运动后立即和运动后30分钟采集血液样本，并将每个受试者的运动条件与对照组进行比较。采用缓冲密度梯度超离心（C-DGUC）分离血浆EVs。分别采用纳米流式细胞术、透射电镜和western blot检测EV的大小、形态和蛋白标记。此外，我们对EV分离物进行了非靶向脂质组学分析。结果：两种运动方式对EVs的大小和浓度均无显著影响（P < 0.05）。然而，我们发现运动后，内皮细胞内的甘油磷脂立即下降（55% vs 49%）。结论：我们的数据表明，尽管阻力运动诱导了内皮细胞脂质组成的一些变化，但含有内皮细胞的脂质似乎不是细胞介导运动诱导适应的关键机制。
The effects of aerobic and resistance exercise on the lipid profile of extracellular vesicles.
Purpose: Lipids are one of the most abundant molecules within extracellular vesicles (EVs) and are important for EV biology and cell signaling. However, very little attention has been given to the role of lipids in the biological function of EVs during exercise. Therefore, we completed a study using a cross-over design to investigate the effects of aerobic and resistance exercise on the lipid profile of EVs.
Methods: Ten healthy participants (23.0 ± 3.6 years) performed an acute bout of aerobic exercise, an acute bout of resistance exercise, as well as a period of rest (control) in a randomized, cross-over design. Blood samples were collected immediately following exercise and 30 min after exercise, and exercise conditions were compared to control within each subject. Plasma EVs were isolated using cushioned-density gradient ultracentrifugation (C-DGUC). The EV size, morphology, and protein markers were examined using nanoflow cytometry, transmission electron microscopy, and western blot, respectively. Additionally, we conducted an untargeted lipidomics analysis on the EV isolate.
Results: Our findings revealed neither exercise modality had a significant effect on the size or concentration of EVs (P > 0.05). However, we found that immediately after exercise there was a decrease in glycerophospholipids within the EVs (55% vs 49%; P < 0.05) in the resistance exercise group compared to the control group, a response not found in the aerobic exercise group.
Conclusion: Our data suggest that although resistance exercise induced some changes in the lipid composition of EVs, the EV-containing lipids do not appear to be a critical mechanism utilized by cells to mediate exercise-induced adaptations.
期刊介绍：
The European Journal of Applied Physiology (EJAP) aims to promote mechanistic advances in human integrative and translational physiology. Physiology is viewed broadly, having overlapping context with related disciplines such as biomechanics, biochemistry, endocrinology, ergonomics, immunology, motor control, and nutrition. EJAP welcomes studies dealing with physical exercise, training and performance. Studies addressing physiological mechanisms are preferred over descriptive studies. Papers dealing with animal models or pathophysiological conditions are not excluded from consideration, but must be clearly relevant to human physiology.
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