溶液条件对伴侣蛋白DNAJB6b自组装的影响。

IF 6.2 2区 化学 Q1 CHEMISTRY, MULTIDISCIPLINARY
Andreas Carlsson, Victoria Maier, Celia Fricke, Tinna Pálmadóttir, Ingemar André, Ulf Olsson, Sara Linse
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引用次数: 0

摘要

伴侣蛋白是维持蛋白质平衡所必需的。它们的主要作用是协助其他蛋白质的折叠,并防止错误折叠蛋白质的聚集。分子伴侣DNAJB6b有效抑制几种肽的淀粉样蛋白形成。这种活性可能依赖于与驱动伴侣自组装成大型胶束状低聚物相同的物理化学性质。因此,我们对不同溶液条件下DNAJB6b的自组装进行了系统的研究。利用互补的生物物理技术,我们探索了聚集数分布和流体动力半径的变化,随着pH值、温度、离子强度或阴离子在Hofmeister系列中的变化。我们发现DNAJB6b在所有测试的溶液条件下都保持其自组装的倾向。当c端结构域展开时,胶束的大小和紧密度会发生变化,尽管折叠的c端结构域不会驱动胶束的形成,这可能归因于连接体区域的疏水相互作用。质谱分析表明,DNAJB6b单体与胶束平衡共存。此外,通过测量胶束在不同温度下的解离速率常数,估计了胶束解离成单体的自由能垒。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effects of solution conditions on the self-assembly of the chaperone protein DNAJB6b.

Chaperone proteins are essential for maintaining proteostasis. Their main role is to assist with the folding of other proteins and to prevent the aggregation of misfolded proteins. The molecular chaperone DNAJB6b efficiently suppresses amyloid formation of several peptides. This activity may rely on the same physicochemical properties as those driving chaperone self-assembly into large micellar-like oligomers. We have therefore undertaken a systematic study of DNAJB6b's self-assembly under different solution conditions. Using complementary biophysical techniques, we probe variations in aggregation number distribution and hydrodynamic radius, upon variation of pH, temperature, ionic strength, or anions across the Hofmeister series. We find that DNAJB6b maintains its propensity to self-assemble under all solution conditions examined. The size and compactness of the micelles change upon unfolding of the C-terminal domain, although a folded C-terminal domain does not drive micelle formation, which can likely be ascribed to hydrophobic interactions in the linker region. Mass photometry reveals that monomers of DNAJB6b coexist at equilibrium with the micelles. Furthermore, the free energy barrier for micelle dissociation into monomers was estimated by measuring dissociation rate constants at different temperatures.

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来源期刊
Communications Chemistry
Communications Chemistry Chemistry-General Chemistry
CiteScore
7.70
自引率
1.70%
发文量
146
审稿时长
13 weeks
期刊介绍: Communications Chemistry is an open access journal from Nature Research publishing high-quality research, reviews and commentary in all areas of the chemical sciences. Research papers published by the journal represent significant advances bringing new chemical insight to a specialized area of research. We also aim to provide a community forum for issues of importance to all chemists, regardless of sub-discipline.
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