由Mn-ZIF-67偶联抗o抗体组成的高性能电化学生物传感器,用于检测大肠杆菌。

IF 6.2 2区 化学 Q1 CHEMISTRY, MULTIDISCIPLINARY
Atqiya Muslihati, Chandra Wulandari, Ni Luh Wulan Septiani, Gilang Gumilar, Agus Subagio, Ida Hamidah, Nugraha Nugraha, Erwin Peiner, Hutomo Suryo Wasisto, Brian Yuliarto
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引用次数: 0

摘要

食品和水中大肠杆菌的检测对公共卫生至关重要,但现有方法往往缺乏灵敏度、速度或现场适用性。在此,我们开发了一种基于mn掺杂Co沸石咪唑酸框架(ZIF-67)的电化学生物传感器,该传感器具有抗o抗体功能化,用于灵敏的大肠杆菌检测。锰的掺入引起相重构、表面积增强和电子转移。抗体偶联调节润湿性,引入酰胺I和酰胺II振动模式,并选择性地阻断细菌结合时的电子转移。该生物传感器的线性范围为10至1010 CFU mL-1,检测限为1 CFU mL-1,优于光学和其他基于金属有机框架的传感器。该方法对非靶菌(沙门氏菌、铜绿假单胞菌、金黄色葡萄球菌)具有良好的鉴别能力,5周内灵敏度保持在80%左右,对自来水中添加的大肠杆菌的回收率为93.10 ~ 107.52%。这项工作显示了现场病原体监测的巨大潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
High-performance electrochemical biosensor comprising Mn-ZIF-67 conjugated with anti-O antibody for Escherichia coli detection.

The detection of Escherichia coli (E. coli) in food and water is critical for public health, yet existing methods often lack sensitivity, speed, or field applicability. Here, we develop an electrochemical biosensor based on Mn-doped Co zeolitic imidazolate framework (ZIF-67) functionalized with anti-O antibody for sensitive E. coli detection. Mn incorporation induces phase reconstruction, surface area enhancement, and electron transfer. Antibody conjugation modulates wettability, introduces amide I and II vibrational modes, and selectively blocks electron transfer upon bacterial binding. The biosensor exhibits a linear range of 10 to 1010 CFU mL-1 with a 1 CFU mL-1 detection limit, outperforming optical and other metal organic framework-based sensors. It can discriminate non-target bacteria (Salmonella, Pseudomonas aeruginosa, Staphylococcus aureus), maintain >80% sensitivity over 5 weeks, and recover 93.10 -107.52% E. coli spiked in tap water. This work suggests the tremendous potential for on-site pathogen monitoring.

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来源期刊
Communications Chemistry
Communications Chemistry Chemistry-General Chemistry
CiteScore
7.70
自引率
1.70%
发文量
146
审稿时长
13 weeks
期刊介绍: Communications Chemistry is an open access journal from Nature Research publishing high-quality research, reviews and commentary in all areas of the chemical sciences. Research papers published by the journal represent significant advances bringing new chemical insight to a specialized area of research. We also aim to provide a community forum for issues of importance to all chemists, regardless of sub-discipline.
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