Atqiya Muslihati, Chandra Wulandari, Ni Luh Wulan Septiani, Gilang Gumilar, Agus Subagio, Ida Hamidah, Nugraha Nugraha, Erwin Peiner, Hutomo Suryo Wasisto, Brian Yuliarto
{"title":"由Mn-ZIF-67偶联抗o抗体组成的高性能电化学生物传感器,用于检测大肠杆菌。","authors":"Atqiya Muslihati, Chandra Wulandari, Ni Luh Wulan Septiani, Gilang Gumilar, Agus Subagio, Ida Hamidah, Nugraha Nugraha, Erwin Peiner, Hutomo Suryo Wasisto, Brian Yuliarto","doi":"10.1038/s42004-025-01703-y","DOIUrl":null,"url":null,"abstract":"<p><p>The detection of Escherichia coli (E. coli) in food and water is critical for public health, yet existing methods often lack sensitivity, speed, or field applicability. Here, we develop an electrochemical biosensor based on Mn-doped Co zeolitic imidazolate framework (ZIF-67) functionalized with anti-O antibody for sensitive E. coli detection. Mn incorporation induces phase reconstruction, surface area enhancement, and electron transfer. Antibody conjugation modulates wettability, introduces amide I and II vibrational modes, and selectively blocks electron transfer upon bacterial binding. The biosensor exhibits a linear range of 10 to 10<sup>10</sup> CFU mL<sup>-1</sup> with a 1 CFU mL<sup>-1</sup> detection limit, outperforming optical and other metal organic framework-based sensors. It can discriminate non-target bacteria (Salmonella, Pseudomonas aeruginosa, Staphylococcus aureus), maintain >80% sensitivity over 5 weeks, and recover 93.10 -107.52% E. coli spiked in tap water. This work suggests the tremendous potential for on-site pathogen monitoring.</p>","PeriodicalId":10529,"journal":{"name":"Communications Chemistry","volume":"8 1","pages":"290"},"PeriodicalIF":6.2000,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12488927/pdf/","citationCount":"0","resultStr":"{\"title\":\"High-performance electrochemical biosensor comprising Mn-ZIF-67 conjugated with anti-O antibody for Escherichia coli detection.\",\"authors\":\"Atqiya Muslihati, Chandra Wulandari, Ni Luh Wulan Septiani, Gilang Gumilar, Agus Subagio, Ida Hamidah, Nugraha Nugraha, Erwin Peiner, Hutomo Suryo Wasisto, Brian Yuliarto\",\"doi\":\"10.1038/s42004-025-01703-y\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The detection of Escherichia coli (E. coli) in food and water is critical for public health, yet existing methods often lack sensitivity, speed, or field applicability. Here, we develop an electrochemical biosensor based on Mn-doped Co zeolitic imidazolate framework (ZIF-67) functionalized with anti-O antibody for sensitive E. coli detection. Mn incorporation induces phase reconstruction, surface area enhancement, and electron transfer. Antibody conjugation modulates wettability, introduces amide I and II vibrational modes, and selectively blocks electron transfer upon bacterial binding. The biosensor exhibits a linear range of 10 to 10<sup>10</sup> CFU mL<sup>-1</sup> with a 1 CFU mL<sup>-1</sup> detection limit, outperforming optical and other metal organic framework-based sensors. It can discriminate non-target bacteria (Salmonella, Pseudomonas aeruginosa, Staphylococcus aureus), maintain >80% sensitivity over 5 weeks, and recover 93.10 -107.52% E. coli spiked in tap water. This work suggests the tremendous potential for on-site pathogen monitoring.</p>\",\"PeriodicalId\":10529,\"journal\":{\"name\":\"Communications Chemistry\",\"volume\":\"8 1\",\"pages\":\"290\"},\"PeriodicalIF\":6.2000,\"publicationDate\":\"2025-10-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12488927/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Communications Chemistry\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://doi.org/10.1038/s42004-025-01703-y\",\"RegionNum\":2,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Communications Chemistry","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1038/s42004-025-01703-y","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
High-performance electrochemical biosensor comprising Mn-ZIF-67 conjugated with anti-O antibody for Escherichia coli detection.
The detection of Escherichia coli (E. coli) in food and water is critical for public health, yet existing methods often lack sensitivity, speed, or field applicability. Here, we develop an electrochemical biosensor based on Mn-doped Co zeolitic imidazolate framework (ZIF-67) functionalized with anti-O antibody for sensitive E. coli detection. Mn incorporation induces phase reconstruction, surface area enhancement, and electron transfer. Antibody conjugation modulates wettability, introduces amide I and II vibrational modes, and selectively blocks electron transfer upon bacterial binding. The biosensor exhibits a linear range of 10 to 1010 CFU mL-1 with a 1 CFU mL-1 detection limit, outperforming optical and other metal organic framework-based sensors. It can discriminate non-target bacteria (Salmonella, Pseudomonas aeruginosa, Staphylococcus aureus), maintain >80% sensitivity over 5 weeks, and recover 93.10 -107.52% E. coli spiked in tap water. This work suggests the tremendous potential for on-site pathogen monitoring.
期刊介绍:
Communications Chemistry is an open access journal from Nature Research publishing high-quality research, reviews and commentary in all areas of the chemical sciences. Research papers published by the journal represent significant advances bringing new chemical insight to a specialized area of research. We also aim to provide a community forum for issues of importance to all chemists, regardless of sub-discipline.