由集成超声光学传感器实现的等距分辨反射模式体内光声显微镜。

IF 9.1 1区 化学 Q1 CHEMISTRY, ANALYTICAL
Fan Yang,Yong Yang,Wei Song,Xiaojing Wu,Siwei Zhu,Jun Liu,Li Zhang,Changyu Shen,Xiaocong Yuan
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引用次数: 0

摘要

由于光声显微镜使用的是窄带宽和光学不透明超声换能器,因此在直接测量宽带光声波时经常面临挑战,这影响了光声体积成像的深度定位精度。为了克服这些挑战,我们提出了一种集成超声光学传感器(IUOS),它将激发激光传输和超声波检测无缝集成在一个设备中。IUOS采用近共径迈克尔逊干涉仪配置,将光声压瞬态转换为反射光束的时变偏振状态,在-6 dB的158 MHz宽带上实现了约127 Pa的灵敏度。此外,传感器允许在532nm波长的激发激光几乎无损传输。当集成到光学分辨率光声显微镜(OR-PAM)时,它允许具有增强轴向分辨率的反射模式体积成像。值得注意的是,该传感器已经成功地实现了斑马鱼体内无标记的体积可视化,大大提高了深度定位精度,突出了其在推进生物医学光声研究方面的巨大潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Isometrically Resolved Reflection-Mode In Vivo Photoacoustic Microscopy Enabled by an Integrated Ultrasonic-Optical Sensor.
Photoacoustic microscopy often faces challenges in direct measurement of broadband photoacoustic waves due to the use of narrow-bandwidth and optical opaque ultrasonic transducers, which compromises the depth localization accuracy in photoacoustic volumetric imaging. To overcome these challenges, we propose an Integrated Ultrasonic-Optical Sensor (IUOS) that seamlessly integrates excitation laser transmission and ultrasonic detection within a single device. Employing a nearly common-path Michelson interferometer configuration, the IUOS transduces photoacoustic pressure transients into time-varying polarization states of the reflected beam, achieving an impressive sensitivity of approximately 127 Pa over a broad bandwidth of 158 MHz at -6 dB. Furthermore, the sensor allows nearly lossless transmission for the excitation laser at a wavelength of 532 nm. When integrated into an optical-resolution photoacoustic microscope (OR-PAM), it allows for reflection-mode volumetric imaging with enhanced axial resolution. Notably, the sensor has successfully achieved label-free volumetric visualization of zebrafish in vivo with greatly improved depth localization accuracy, highlighting its considerable potential to advance biomedical photoacoustic research.
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来源期刊
ACS Sensors
ACS Sensors Chemical Engineering-Bioengineering
CiteScore
14.50
自引率
3.40%
发文量
372
期刊介绍: ACS Sensors is a peer-reviewed research journal that focuses on the dissemination of new and original knowledge in the field of sensor science, particularly those that selectively sense chemical or biological species or processes. The journal covers a broad range of topics, including but not limited to biosensors, chemical sensors, gas sensors, intracellular sensors, single molecule sensors, cell chips, and microfluidic devices. It aims to publish articles that address conceptual advances in sensing technology applicable to various types of analytes or application papers that report on the use of existing sensing concepts in new ways or for new analytes.
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