Fan Yang,Yong Yang,Wei Song,Xiaojing Wu,Siwei Zhu,Jun Liu,Li Zhang,Changyu Shen,Xiaocong Yuan
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Isometrically Resolved Reflection-Mode In Vivo Photoacoustic Microscopy Enabled by an Integrated Ultrasonic-Optical Sensor.
Photoacoustic microscopy often faces challenges in direct measurement of broadband photoacoustic waves due to the use of narrow-bandwidth and optical opaque ultrasonic transducers, which compromises the depth localization accuracy in photoacoustic volumetric imaging. To overcome these challenges, we propose an Integrated Ultrasonic-Optical Sensor (IUOS) that seamlessly integrates excitation laser transmission and ultrasonic detection within a single device. Employing a nearly common-path Michelson interferometer configuration, the IUOS transduces photoacoustic pressure transients into time-varying polarization states of the reflected beam, achieving an impressive sensitivity of approximately 127 Pa over a broad bandwidth of 158 MHz at -6 dB. Furthermore, the sensor allows nearly lossless transmission for the excitation laser at a wavelength of 532 nm. When integrated into an optical-resolution photoacoustic microscope (OR-PAM), it allows for reflection-mode volumetric imaging with enhanced axial resolution. Notably, the sensor has successfully achieved label-free volumetric visualization of zebrafish in vivo with greatly improved depth localization accuracy, highlighting its considerable potential to advance biomedical photoacoustic research.
期刊介绍:
ACS Sensors is a peer-reviewed research journal that focuses on the dissemination of new and original knowledge in the field of sensor science, particularly those that selectively sense chemical or biological species or processes. The journal covers a broad range of topics, including but not limited to biosensors, chemical sensors, gas sensors, intracellular sensors, single molecule sensors, cell chips, and microfluidic devices. It aims to publish articles that address conceptual advances in sensing technology applicable to various types of analytes or application papers that report on the use of existing sensing concepts in new ways or for new analytes.