相敏表面等离子体共振成像与偏振调制和斯托克斯矢量测量。

IF 9.1 1区 化学 Q1 CHEMISTRY, ANALYTICAL
Baiqi Cui,Xiaoyin Liu,Jinbiao Ma,Jingyu Wu,Yunxiao Wang,Haiying Ding,Di Wang,Qingjun Liu,Fenni Zhang
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引用次数: 0

摘要

相敏表面等离子体共振(P-SPR)技术与传统的基于强度的SPR方法相比具有更高的灵敏度。然而,目前的实现,如基于干涉和基于偏振的相位测量,经常面临光学复杂性和相位分辨率之间的权衡,并且通常无法获得完整的偏振信息。在这里,我们报告了一个紧凑和通用的Stokes矢量偏振SPR成像(Sp-SPRi)系统,该系统通过对偏振调制后的成像光进行全Stokes矢量分析来解决这些限制。这种分析可以同时获得相和多极化参数,以增强分子测量。Sp-SPRi系统具有1.80 × 10-7折射率单位(RIU)的高相位灵敏度,支持生物分子相互作用的动力学、无标记检测和定量。我们通过蛋白质结合、小分子相互作用的动力学测量和单细胞的原位糖蛋白测量来证明其性能。凭借其简化的光学配置,微流体集成和高通量成像能力,Sp-SPRi为多维生物传感提供了强大的平台。这项工作拓宽了P-SPR的分析范围,为即时诊断、早期疾病检测和药物筛选的应用提供了一种可靠且易于获取的方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Phase-Sensitive Surface Plasmon Resonance Imaging with Polarization Modulation and Stokes Vector Measurement.
Phase-sensitive surface plasmon resonance (P-SPR) techniques offer superior sensitivity compared to conventional intensity-based SPR methods. However, current implementations, such as interference-based and polarization-based phase measurements, often face trade-offs between optical complexity and phase resolution, and typically lack access to full polarization information. Here, we report a compact and versatile Stokes vector-based polarization SPR imaging (Sp-SPRi) system that addresses these limitations through full Stokes vector analysis of imaging light after polarization modulation. This analysis enables the simultaneous acquisition of phase and multiple polarization parameters for enhanced molecular measurement. The Sp-SPRi system achieves a high phase sensitivity of 1.80 × 10-7 refractive index units (RIU), supporting kinetic, label-free detection and quantification of biomolecular interactions. We demonstrate its performance through kinetic measurements of protein binding, small molecule interactions, and in situ glycoprotein measurement of single cells. With its simplified optical configuration, microfluidic integration, and high-throughput imaging capabilities, Sp-SPRi provides a powerful platform for multidimensional biosensing. This work broadens the analytical scope of P-SPR, offering a robust and accessible approach for applications in point-of-care diagnostics, early disease detection, and drug screening.
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来源期刊
ACS Sensors
ACS Sensors Chemical Engineering-Bioengineering
CiteScore
14.50
自引率
3.40%
发文量
372
期刊介绍: ACS Sensors is a peer-reviewed research journal that focuses on the dissemination of new and original knowledge in the field of sensor science, particularly those that selectively sense chemical or biological species or processes. The journal covers a broad range of topics, including but not limited to biosensors, chemical sensors, gas sensors, intracellular sensors, single molecule sensors, cell chips, and microfluidic devices. It aims to publish articles that address conceptual advances in sensing technology applicable to various types of analytes or application papers that report on the use of existing sensing concepts in new ways or for new analytes.
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