{"title":"新型生物活性万能键与钙盐单体对龋的矿化、抗脱矿和抗菌作用。","authors":"Bayarchimeg Altankhishig, Yasuhiro Matsuda, Yaxin Rao, Takashi Saito","doi":"10.3389/fdmed.2025.1633158","DOIUrl":null,"url":null,"abstract":"<p><p>This study investigated the mineralization, anti-demineralization, and antibacterial properties of a novel bioactive universal adhesive containing the calcium salt of 4-methacryloxyethyl trimellitate acid (CMET). Four adhesives were evaluated: Bioactive Universal Bond with CMET (BA), Clearfil Universal Bond Quick (CU), Prime & Bond Universal (PB), and Scotchbond Universal Plus Adhesive (SUB). To assess mineralization properties, BA specimens were immersed in simulated body fluid (SBF) at 37°C, in line with ISO 23317, and the induced crystals were analyzed using scanning electron microscopy and energy dispersive x-ray spectroscopy. Anti-demineralization was evaluated by applying each adhesive to polished bovine dentin, followed by a one-week pH cycling protocol. Transverse microradiography (TMR) was used to quantify the integrated mineral loss. The antibacterial activity was assessed using eluates prepared by immersing the cured specimens in distilled water for seven days, followed by serial dilutions (10%, 5%, 1%, and 0.5%). These eluates were incubated with <i>Streptococcus mutans</i>, <i>Actinomyces viscosus</i>, and <i>Lactobacillus casei</i> for 24 h at 37°C. Viable bacterial counts were determined using the quantitative polymerase chain reaction following propidium monoazide treatment. BA exhibited distinct mineralization in SBF, likely attributable to CMET, and was characterized by the formation of octacalcium-phosphate-like crystals. TMR analysis showed that BA significantly suppressed demineralization at the dentin-material interface in relation to the other adhesives. In antibacterial assays, 5% and 10% BA eluates markedly suppressed the growth of <i>S. mutans</i> and <i>A. viscosus,</i> whereas CU, PB, and SUB exhibited inhibitory effects at only 10% concentration for <i>S. mutans</i> and had no impact on <i>A. viscosus</i>. Notably, only the 10% BA eluate significantly inhibited <i>L. casei</i> growth. Despite the limitations of the <i>in vitro</i> experiments, these findings suggest that BA possesses multifunctional properties, supporting its potential as an effective adhesive system for the prevention and treatment of caries. Furthermore, its demonstrated bioactivity suggests promising applications across various biomedical fields, such as antibacterial coatings for medical devices, bone-regenerative scaffolds, and bioactive interfaces in tissue engineering and regenerative medicine.</p>","PeriodicalId":73077,"journal":{"name":"Frontiers in dental medicine","volume":"6 ","pages":"1633158"},"PeriodicalIF":1.8000,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12477213/pdf/","citationCount":"0","resultStr":"{\"title\":\"Mineralization, anti-demineralization, and antibacterial effects of novel Bioactive Universal Bond with calcium salt monomers on dental caries.\",\"authors\":\"Bayarchimeg Altankhishig, Yasuhiro Matsuda, Yaxin Rao, Takashi Saito\",\"doi\":\"10.3389/fdmed.2025.1633158\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>This study investigated the mineralization, anti-demineralization, and antibacterial properties of a novel bioactive universal adhesive containing the calcium salt of 4-methacryloxyethyl trimellitate acid (CMET). Four adhesives were evaluated: Bioactive Universal Bond with CMET (BA), Clearfil Universal Bond Quick (CU), Prime & Bond Universal (PB), and Scotchbond Universal Plus Adhesive (SUB). To assess mineralization properties, BA specimens were immersed in simulated body fluid (SBF) at 37°C, in line with ISO 23317, and the induced crystals were analyzed using scanning electron microscopy and energy dispersive x-ray spectroscopy. Anti-demineralization was evaluated by applying each adhesive to polished bovine dentin, followed by a one-week pH cycling protocol. Transverse microradiography (TMR) was used to quantify the integrated mineral loss. The antibacterial activity was assessed using eluates prepared by immersing the cured specimens in distilled water for seven days, followed by serial dilutions (10%, 5%, 1%, and 0.5%). These eluates were incubated with <i>Streptococcus mutans</i>, <i>Actinomyces viscosus</i>, and <i>Lactobacillus casei</i> for 24 h at 37°C. Viable bacterial counts were determined using the quantitative polymerase chain reaction following propidium monoazide treatment. BA exhibited distinct mineralization in SBF, likely attributable to CMET, and was characterized by the formation of octacalcium-phosphate-like crystals. TMR analysis showed that BA significantly suppressed demineralization at the dentin-material interface in relation to the other adhesives. In antibacterial assays, 5% and 10% BA eluates markedly suppressed the growth of <i>S. mutans</i> and <i>A. viscosus,</i> whereas CU, PB, and SUB exhibited inhibitory effects at only 10% concentration for <i>S. mutans</i> and had no impact on <i>A. viscosus</i>. Notably, only the 10% BA eluate significantly inhibited <i>L. casei</i> growth. Despite the limitations of the <i>in vitro</i> experiments, these findings suggest that BA possesses multifunctional properties, supporting its potential as an effective adhesive system for the prevention and treatment of caries. 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引用次数: 0
摘要
研究了含4-甲基丙烯氧乙基三ellitate acid (CMET)钙盐的新型生物活性通用胶粘剂的矿化、抗脱矿和抗菌性能。对四种胶粘剂进行了评价:Bioactive Universal Bond with CMET (BA)、Clearfil Universal Bond Quick (CU)、Prime & Bond Universal (PB)和Scotchbond Universal Plus Adhesive (SUB)。为了评估BA样品的矿化性质,将BA样品浸入模拟体液(SBF)中,温度为37°C,符合ISO 23317标准,并使用扫描电镜和能量色散x射线能谱分析诱导晶体。通过将每种粘合剂应用于抛光的牛牙本质,然后进行一周的pH循环方案来评估抗脱矿性。横向显微放射照相(TMR)用于量化综合矿物质损失。将固化后的标本在蒸馏水中浸泡7天,然后依次稀释(10%、5%、1%和0.5%),以洗脱液评估其抗菌活性。这些洗脱液与变形链球菌、粘放线菌和干酪乳杆菌在37℃下孵育24小时。采用单叠氮丙啶处理后的定量聚合酶链反应测定活菌数。BA在SBF中表现出明显的矿化,可能与CMET有关,其特征是形成八磷酸钙样晶体。TMR分析表明,与其他黏合剂相比,BA明显抑制了牙本质-材料界面的脱矿。在抗菌实验中,5%和10% BA洗脱液能明显抑制变形链球菌和粘单胞菌的生长,而只有10%浓度的CU、PB和SUB对变形链球菌有抑制作用,对粘单胞菌没有影响。值得注意的是,只有10%的BA洗脱液能显著抑制干酪乳杆菌的生长。尽管体外实验存在局限性,但这些发现表明BA具有多功能特性,支持其作为预防和治疗龋齿的有效粘接系统的潜力。此外,其已证明的生物活性表明其在各种生物医学领域的应用前景广阔,如医疗设备的抗菌涂层、骨再生支架、组织工程和再生医学中的生物活性界面。
Mineralization, anti-demineralization, and antibacterial effects of novel Bioactive Universal Bond with calcium salt monomers on dental caries.
This study investigated the mineralization, anti-demineralization, and antibacterial properties of a novel bioactive universal adhesive containing the calcium salt of 4-methacryloxyethyl trimellitate acid (CMET). Four adhesives were evaluated: Bioactive Universal Bond with CMET (BA), Clearfil Universal Bond Quick (CU), Prime & Bond Universal (PB), and Scotchbond Universal Plus Adhesive (SUB). To assess mineralization properties, BA specimens were immersed in simulated body fluid (SBF) at 37°C, in line with ISO 23317, and the induced crystals were analyzed using scanning electron microscopy and energy dispersive x-ray spectroscopy. Anti-demineralization was evaluated by applying each adhesive to polished bovine dentin, followed by a one-week pH cycling protocol. Transverse microradiography (TMR) was used to quantify the integrated mineral loss. The antibacterial activity was assessed using eluates prepared by immersing the cured specimens in distilled water for seven days, followed by serial dilutions (10%, 5%, 1%, and 0.5%). These eluates were incubated with Streptococcus mutans, Actinomyces viscosus, and Lactobacillus casei for 24 h at 37°C. Viable bacterial counts were determined using the quantitative polymerase chain reaction following propidium monoazide treatment. BA exhibited distinct mineralization in SBF, likely attributable to CMET, and was characterized by the formation of octacalcium-phosphate-like crystals. TMR analysis showed that BA significantly suppressed demineralization at the dentin-material interface in relation to the other adhesives. In antibacterial assays, 5% and 10% BA eluates markedly suppressed the growth of S. mutans and A. viscosus, whereas CU, PB, and SUB exhibited inhibitory effects at only 10% concentration for S. mutans and had no impact on A. viscosus. Notably, only the 10% BA eluate significantly inhibited L. casei growth. Despite the limitations of the in vitro experiments, these findings suggest that BA possesses multifunctional properties, supporting its potential as an effective adhesive system for the prevention and treatment of caries. Furthermore, its demonstrated bioactivity suggests promising applications across various biomedical fields, such as antibacterial coatings for medical devices, bone-regenerative scaffolds, and bioactive interfaces in tissue engineering and regenerative medicine.