SIRT1 rs12415800和SIRT6 rs350844多态性与先兆子痫的关联：一项病例对照研究

IF 2.9 4区医学 Q2 OBSTETRICS & GYNECOLOGY

Pregnancy Hypertension-An International Journal of Womens Cardiovascular Health Pub Date : 2025-09-29 DOI:10.1016/j.preghy.2025.101259

Chao Zhu , Longqiang Xu , Fumin Zheng , Jingli Wang , Yuanxuan Ma , Jialiang Guan , Ru Zhang , Shiguo Liu

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引用次数: 0

摘要

背景：SIRT1和SIRT6是Sirtuins （SIRTs）家族的关键成员，在调节细胞代谢和存活中起着至关重要的作用，对衰老和代谢性疾病具有有益作用，可能通过抗氧化和炎症反应调节胎盘细胞衰老表型，参与子痫前期（PE）的发病机制。本研究旨在探讨中国汉族女性SIRT1和SIRT6多态性与PE的关系。方法：将619名健康孕妇作为对照组，699名PE孕妇作为病例。从外周血样本中提取DNA，通过实时定量聚合酶链反应（PCR）对SIRT1中的rs12415800和SIRT6中的rs350844进行基因分型。通过比较两组的基因型和等位基因频率，评估SIRT1和SIRT6多态性与PE之间的关系。结果：SIRT1 rs12415800多态性可能与PE发生风险相关，AA基因型比AG和GG基因型发生PE的风险更低（基因型χ2 = 6.612, P = 0.037；等位基因χ2 = 1.279, P = 0.258; OR = 0.720, 95% CI = 0.548 ~ 0.951, P = 0.002）。亚组分析（早发性与晚发性PE，轻度与重度PE）进一步支持这些发现，显示出一致甚至更明显的关联。结论：我们的研究结果表明，SIRT1基因变异rs12415800可能与中国汉族女性PE风险有关，而AA基因型使风险降低。进一步的研究需要在其他人群和更大的前瞻性研究中验证这些发现。

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Association of SIRT1 rs12415800 and SIRT6 rs350844 polymorphisms with preeclampsia: A case-control study

Background

SIRT1 and SIRT6, key members of the Sirtuins (SIRTs) family, play crucial roles in regulating cell metabolism and survival, providing beneficial effects against aging and metabolic diseases, which may be involved in the pathogenesis of preeclampsia (PE) by modulating cell senescence phenotypes in the placenta through antioxidant and inflammatory responses. This study aimed to investigate the association of SIRT1 and SIRT6 polymorphisms with PE in Chinese Han women.

Method

A total of 619 healthy pregnant women were enrolled as controls, and 699 pregnant women with PE as cases. DNA was extracted from peripheral blood samples and was used for genotyping rs12415800 in SIRT1 and rs350844 in SIRT6 via real-time quantitative polymerase chain reaction (PCR). The relationship between SIRT1 and SIRT6 polymorphisms and PE was assessed by comparing genotypic and allelic frequencies between the two groups.

Results

The results indicated that the SIRT1 rs12415800 polymorphism may be associated with PE risk and the AA genotypes was associated with a lower risk of developing PE compared to the AG and GG (genotype χ2 = 6.612, P = 0.037; allele χ2 = 1.279, P = 0.258; OR = 0.720, 95 % CI = 0.548 ∼ 0.951, P = 0.002). Subgroup analyses (early-onset vs. late-onset PE, mild vs. severe PE) further supported these findings, showing consistent or even more pronounced associations.

Conclusion

Our findings suggest that the genetic variant rs12415800 in SIRT1 may be linked to PE risk in Chinese Han women, with the AA genotype conferring a reduced risk. Further research is warranted to validate these findings in other populations and larger prospective studies.

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来源期刊

Pregnancy Hypertension-An International Journal of Womens Cardiovascular Health OBSTETRICS & GYNECOLOGYPERIPHERAL VASCULAR-PERIPHERAL VASCULAR DISEASE

CiteScore

4.90

自引率

0.00%

发文量

127

期刊介绍： Pregnancy Hypertension: An International Journal of Women''s Cardiovascular Health aims to stimulate research in the field of hypertension in pregnancy, disseminate the useful results of such research, and advance education in the field. We publish articles pertaining to human and animal blood pressure during gestation, hypertension during gestation including physiology of circulatory control, pathophysiology, methodology, therapy or any other material relevant to the relationship between elevated blood pressure and pregnancy. The subtitle reflects the wider aspects of studying hypertension in pregnancy thus we also publish articles on in utero programming, nutrition, long term effects of hypertension in pregnancy on cardiovascular health and other research that helps our understanding of the etiology or consequences of hypertension in pregnancy. Case reports are not published unless of exceptional/outstanding importance to the field.