葡萄和丹参在早期白粉病感染过程中的双单核基因表达图谱。

IF 3.4 3区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
Maria-Sole Bonarota, Jadran F Garcia, Mélanie Massonnet, Mirella Zaccheo, Rosa Figueroa-Balderas, Noé Cochetel, Dario Cantu
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引用次数: 0

摘要

我们应用单核转录组学研究了白粉病病原菌(erysipphe necator)在感染后1天和5天感染葡萄叶片的情况,包括对照和3个生物重复。我们制作了一个包含超过10万个细胞核的葡萄藤叶片图谱,以及一个包含超过3000个细胞核的病原体图谱。我们成功地注释了所有主要的葡萄细胞类型,包括叶肉细胞、表皮细胞、韧皮部和木质部薄壁细胞、伴随细胞和保护细胞。我们鉴定了大肠杆菌的关键结构,包括附着胞、吸器和菌丝,并为这两个物种提供了新的细胞类型标记。我们揭示了E. necator中结构特异性的基因表达程序,为未来真菌发育和毒力机制的研究奠定了基础。在宿主中,我们发现了防御相关基因在空间上的不同表达模式。随着感染的进展,我们观察到涉及多种细胞类型(主要是表皮细胞和叶肉细胞)的协调免疫反应的激活。高维加权基因共表达网络分析确定了与细胞类型特异性信号传导和防御反应相关的关键枢纽和网络。我们描述了模式触发免疫和效应触发免疫的空间分离,支持模式触发免疫在病原体接触部位被激活,效应触发免疫在周围组织中被诱导的模型。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Dual Single-Nucleus Gene Expression Atlas of Grapevine and Erysiphe necator During Early Powdery Mildew Infection.

We applied single-nucleus transcriptomics to study how Erysiphe necator (causal agent of powdery mildew) infects grapevine leaves at one- and five- days post infection, including controls and three biological replicates. We generated a grapevine leaf atlas encompassing over 100,000 nuclei, and a pathogen atlas of more than 3,000 nuclei. We successfully annotated all major grapevine cell types, including mesophyll, epidermis, phloem and xylem parenchyma, companion cells, and guard cells. We identified key E. necator structures, including appressoria, haustoria, and hyphae, and provided a list of novel cell type markers for both species. We reveal structure-specific gene expression programs in E. necator laying a foundation for future studies of fungal development and virulence mechanisms. In the host, we identified spatially distinct expression patterns of defense-related genes. As the infection progressed, we observed the activation of a coordinated immune response involving multiple cell types, mainly epidermal and mesophyll cells. High-dimensional weighted gene co-expression network analysis identified key hubs and networks associated with cell type-specific signaling and defense response. We describe a spatial separation of pattern- and effector- triggered immunity, supporting a model in which pattern-triggered immunity is activated at the site of pathogen contact and effector-triggered immunity is induced in surrounding tissue.

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来源期刊
Molecular Plant-microbe Interactions
Molecular Plant-microbe Interactions 生物-生化与分子生物学
CiteScore
7.00
自引率
2.90%
发文量
250
审稿时长
3 months
期刊介绍: Molecular Plant-Microbe Interactions® (MPMI) publishes fundamental and advanced applied research on the genetics, genomics, molecular biology, biochemistry, and biophysics of pathological, symbiotic, and associative interactions of microbes, insects, nematodes, or parasitic plants with plants.
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